LOCUS       sequence-213178-        7827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-PTK2-C-10
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    4555..4676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4592..4611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4646..4665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4683..5138)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4820..4841)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5032..5051
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5165..5269
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5271..5628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5271..5462
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5294..5314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5479..5614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5663..6457
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5663..6457
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5717..5736)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6327..6346
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6645..6664)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6689..6736
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7065..7653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7554..7573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc
      601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      721 ctgaccactg cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa
      781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      901 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg
      961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat
     1021 attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1201 cattctggat tgcctgacaa tgccagacga tccggactca gatctagcgg tggcagtgga
     1261 gcagctgctt accttgaccc caacttgaat cacacaccaa attcgagtac taagactcac
     1321 ctgggtactg gtatggaacg ttctcctggt gcaatggagc gagtattaaa ggtctttcat
     1381 tattttgaaa gcaatagtga gccaaccacc tgggccagta ttatcaggca tggagatgct
     1441 actgatgtca ggggcatcat tcagaagata gtggacagtc acaaagtaaa gcatgtggcc
     1501 tgctatggat tccgcctcag tcacctgcgg tcagaggagg ttcactggct tcacgtggat
     1561 atgggcgtct ccagtgtgag ggagaagtat gagcttgctc acccaccaga ggagtggaaa
     1621 tatgaattga gaattcgtta tttgccaaaa ggatttctaa accagtttac tgaagataag
     1681 ccaactttga atttcttcta tcaacaggtg aagagcgatt atatgttaga gatagctgat
     1741 caagtggacc aggaaattgc tttgaagttg ggttgtctag aaatacggcg atcatactgg
     1801 gagatgcggg gcaatgcact agaaaagaag tctaactatg aagtattaga aaaagatgtt
     1861 ggtttaaagc gattttttcc taagagttta ctggattctg tcaaggccaa aacactaaga
     1921 aaactgatcc aacaaacatt tagacaattt gccaacctta atagagaaga aagtattctg
     1981 aaattctttg agatcctgtc tccagtctac agatttgata aggaatgctt caagtgtgct
     2041 cttggttcaa gctggattat ttcagtggaa ctggcaatcg gcccagaaga aggaatcagt
     2101 tacctaacgg acaagggctg caatcccaca catcttgctg acttcactca agtgcaaacc
     2161 attcagtatt caaacagtga agacaaggac agaaaaggaa tgctacaact aaaaatagca
     2221 ggtgcacccg agcctctgac agtgacggca ccatccctaa ccattgcgga gaatatggct
     2281 gacctaatag atgggtactg ccggctggtg aatggaacct cgcagtcatt tatcatcaga
     2341 cctcagaaag aaggtgaacg ggctttgcca tcaataccaa agttggccaa cagcgaaaag
     2401 caaggcatgc ggacacacgc cgtctctgtg tcagaaacag atgattatgc tgagattata
     2461 gatgaagaag atacttacac catgccctca accagggatt atgagattca aagagaaaga
     2521 atagaacttg gacgatgtat tggagaaggc caatttggag atgtacatca aggcatttat
     2581 atgagtccag agaatccagc tttggcggtt gcaattaaaa catgtaaaaa ctgtacttcg
     2641 gacagcgtga gagagaaatt tcttcaagaa gccttaacaa tgcgtcagtt tgaccatcct
     2701 catattgtga agctgattgg agtcatcaca gagaatcctg tctggataat catggagctg
     2761 tgcacacttg gagagctgag gtcatttttg caagtaagga aatacagttt ggatctagca
     2821 tctttgatcc tgtatgccta tcagcttagt acagctcttg catatctaga gagcaaaaga
     2881 tttgtacaca gggacattgc tgctcggaat gttctggtgt cctcaaatga ttgtgtaaaa
     2941 ttaggagact ttggattatc ccgatatatg gaagatagta cttactacaa agcttccaaa
     3001 ggaaaattgc ctattaaatg gatggctcca gagtcaatca attttcgacg ttttacctca
     3061 gctagtgacg tatggatgtt tggtgtgtgt atgtgggaga tactgatgca tggtgtgaag
     3121 ccttttcaag gagtgaagaa caatgatgta atcggtcgaa ttgaaaatgg ggaaagatta
     3181 ccaatgcctc caaattgtcc tcctaccctc tacagcctta tgacgaaatg ctgggcctat
     3241 gaccccagca ggcggcccag gtttactgaa cttaaagctc agctcagcac aatcctggag
     3301 gaagagaagg ctcagcaaga agagcgcatg aggatggagt ccagaagaca ggccacagtg
     3361 tcctgggact ccggagggtc tgatgaagca ccgcccaagc ccagcagacc gggttatccc
     3421 agtccgaggt ccagcgaagg attttatccc agcccacagc acatggtaca aaccaatcat
     3481 taccaggttt ctggctaccc tggttcacat ggaatcacag ccatggctgg cagcatctat
     3541 ccaggtcagg catctctttt ggaccaaaca gattcatgga atcatagacc tcaggagata
     3601 gcaatgtggc agcccaatgt ggaggactct acagtattgg acctgcgagg gattgggcaa
     3661 gtgttgccaa cccatctgat ggaagagcgt ctaatccgac agcaacagga aatggaagaa
     3721 gatcagcgct ggctggaaaa agaggaaaga tttctgaaac ctgatgtgag actctctcga
     3781 ggcagtattg acagggagga tggaagtctt cagggtccga ttggaaacca acatatatat
     3841 cagcctgtgg gtaaaccaga tcctgcagct ccaccaaaga aaccgcctcg ccctggagct
     3901 cccggtcatc tgggaagcct tgccagcctc agcagccctg ctgacagcta caacgagggt
     3961 gtcaagccat ggaggcttca gccccaggaa atcagccccc ctcctactgc caacctggac
     4021 cggtcgaatg ataaggtgta cgagaatgtg acgggcctgg tgaaagctgt catcgagatg
     4081 tccagtaaaa tccagccagc cccaccagag gagtatgtcc ctatggtgaa ggaagtcggc
     4141 ttggccctga ggacattatt ggccactgtg gatgagacca ttcccctcct accagccagc
     4201 acccaccgag agattgagat ggcacagaag ctattgaact ctgacctggg tgagctcatc
     4261 aacaagatga aactggccca gcagtatgtc atgaccagcc tccagcaaga gtacaaaaag
     4321 caaatgctga ctgctgctca cgccctggct gtggatgcca aaaacttact cgatgtcatt
     4381 gaccaagcaa gactgaaaat gcttgggcag acgagaccac actgaggatc caccggatct
     4441 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4501 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4561 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4621 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     4681 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4741 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4801 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4861 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4921 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4981 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     5041 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     5101 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     5161 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5221 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     5281 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5341 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5401 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5461 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5521 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5581 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     5641 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5701 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5761 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5821 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     5881 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5941 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     6001 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     6061 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     6121 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     6181 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     6241 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     6301 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     6361 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6421 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6481 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6541 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6601 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     6661 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     6721 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     6781 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     6841 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     6901 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6961 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7021 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7081 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7141 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7201 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7261 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7321 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7381 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7441 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7501 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7561 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7621 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7681 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7741 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     7801 agttcatagc ccatatatgg agttccg
//
