LOCUS       sequence-213179-        6673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6673)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..6673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             249..926
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3097..3218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3134..3153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3188..3207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3225..3680)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3362..3383)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3574..3593
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3707..3811
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3813..4170
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3813..4004
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3836..3856)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4021..4156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4205..4999
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4205..4999
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4259..4278)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4869..4888
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5187..5206)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5231..5278
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5607..6195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6096..6115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6370..6673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg
      301 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
      361 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
      421 ccactgcatt ccattacggc aacagggtat tcgccaaata tccagacaac atacaagact
      481 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
      541 ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc tataataaag
      601 ttcgatttta tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga
      661 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc
      721 atatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
      781 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga
      841 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
      901 ctggattgcc tgacaatgcc agacgatccg gactcagatc tcgagtgagc gctctggcct
      961 cccgcgctac cgccatgcag tcctccggct ccttcaacta cgcgcgccct aagcagttca
     1021 tcgcggcgca gaacctgggt cccgcatcgg ggctgcccac gccaacctcc agccccagct
     1081 cctccagctt gccgtcacca ttgtccccca cgcccaggcc cttcggccgc gcgcccgggc
     1141 cgcccttcgt ggaacccgag gccatgtggg gcccgtcctc gccctcgccg ccaccgccgc
     1201 cacctccggt cttcagcccc tctgcagcct acccggtgcc cgacgtgttc ccactgccac
     1261 cgccgccgcc accgctacct agctccacct cacactgcgc ctcgcccgcc cgcttcggcc
     1321 ccagccagac gcccgcagcc ttcctcagcg ccctgctgcc ctctcagcca ccgcctgtcg
     1381 ctgtcaacgc cctggggctg cccaagggag tcacccccgc ggggttccca aagaagtcca
     1441 gtagaactgc tagaattgcc tctgatgagg agattcaagg cacaaaggat gctgtcatcc
     1501 aagacctgga acggaagctt cgcttcaagg aggaccttct gaacaatggc caaccgaggc
     1561 taacctatga ggaaagaatg gctcgccgcc tgcttggagc cgacagcgca aacgtcttca
     1621 acatccagga gccagaggaa acggcagcca atcaggagta caaagtctct agctgcgagc
     1681 agaggctgat tagcgagatt gaatacaggc tggagcgctc ccctgtggat gagtcgggag
     1741 acgaggtgca ggatccagat gtgcctgtgg agaacgcaac agctcccttc tttgagatga
     1801 agctgaaaca ctacaagatc tttgagggga tgccggtgac tttcacgtgt cgagtggctg
     1861 ggaatccaaa gccaaagatc tattggttta aagatgggaa gcagatttct ccgaagagcg
     1921 atcactacac cattcagaga gaccttgatg ggacctgctc tctccacacc acggcctcta
     1981 ccctagacga cgatgggaac tacaccatca tggctgccaa ccctcagggt cgcgtcagtt
     2041 gtacaggaag gctaatggta caggctgtca accaaagagg ccgcagtccc cgctctccct
     2101 caggccatcc tcatgccaga aggcctcgct ctcgatcacg ggacagtgga gatgaaaacg
     2161 agcccattca ggagcgattc ttcagacctc acttcctgca ggctcctgga gacctgaccg
     2221 ttcaggaagg caagctctgc aggatggact gcaaggtcag tgggttacca accccagatc
     2281 tcagctggca actagacgga aagcccatac gccccgacag tgctcacaag atgctggtcc
     2341 gtgagaatgg ggtccactcc ctcattatag agccagtcac gtcccgggac gccggcatct
     2401 acacatgtat tgccaccaac agagcaggac agaactcgtt taacctggag cttgtggttg
     2461 ctgctaagga agcacacaag gcccctgtgt ttatggagaa gctacagaac acgggggttg
     2521 ctgatggata cccagtgcgg ctagaatgcc gtgtctccgg agtgccgcca cctcagatat
     2581 tttggaagaa agaaaatgaa tcgctcactc atagcactga gcgagtaagc atgcaccagg
     2641 ataatcatgg ctacatctgc ctgctcatcc agggagccac aaaggaagac gctgggtggt
     2701 acactgtgtc tgccaagaac gaagcaggca ttgtgtcctg cactgcccgg ctggatgtct
     2761 acacccagtg gcaccagcag ccacagacca ccaagccaaa aaaagtacgg ccctcggcca
     2821 gtcgctacgc agcactttcg gaccagggac tagacatcaa agccgctttc caacctgaag
     2881 ccagcccatc tcacctgact ctgaacagcg gcttggtaga aagtgaagac ctgtgagaat
     2941 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     3001 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3061 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3121 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3181 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     3241 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3301 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3361 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3421 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3481 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3541 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3601 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3661 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3781 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     3841 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4081 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4141 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     4201 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4261 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4321 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4381 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4441 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4501 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4561 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4621 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4681 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     4741 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4801 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4861 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4921 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4981 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5041 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5161 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     5221 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     5281 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5341 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5401 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     5461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6301 gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6361 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6421 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     6661 cgctattacc atg
//
