LOCUS       sequence-213188-        8181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g00430 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g00430-bio-His
FEATURES             Location/Qualifiers
     source          1..8181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3345..3362
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3414..3469
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3468..3487)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5705..5723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5791..5895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5896..5964,5965..6756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6114..6133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6927..7515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7416..7435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7669..7686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7803..7824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7839..7844,7845..7862,7863..7869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7877..7893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7882..7904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7901..7917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7901..7917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 ctgtgatcac ccctcccaac gtgggcgacg ccgacctgaa ggacctggcc acccccgacg
     1261 agagcgagac aaacgaggcc ctgcccgacc agaccgaccc caacgccaat accgccacca
     1321 gcaccaacga cgccgacgag cctcagaccg gcggcatcct gagcatgctg gaaatgggca
     1381 gagtgaacga cggcgagaag cagaagatcg agatcacccc caaggcccgg gaaaccagct
     1441 acgaagtgaa agtgaccaac atcatgaaga aggacaccag cacaatcacc ccccaggaaa
     1501 ccgacagccc caacaagccc agacccagcg gcaacctgcg gcggatcatc agcgagacac
     1561 tggccgagca gagcctggac gccgagaaca agcccaacaa ggacgccccc aaagaggaaa
     1621 cccggaacaa gcccgagaaa gaagccgtga tcgcccccgt ggaccccacc gccatgaaca
     1681 tcctggaacc cgagcagatc gacaaccccg ccacccagct gggcgccgat ggaaagaccc
     1741 ctcccgccct gttcgaggac gaccccccca tcgacgtgat cgtggaccac aacctggaat
     1801 tcggcctgat caacgtgttc cccctgagca agctgtacag cgcctacctg cacggcgtga
     1861 cccggcacaa ggatttcttc gacctgcccc ccgacacagc cggcgacgag gtgtccagcc
     1921 acccctacaa cggcaagatc ctggaagccc tgaacgccct ggtggaccag atcaagttca
     1981 accacgaccg gatcaacaag atgaacaacg agctgatcat cagccagcgg gacatcacca
     2041 gcgtgtccaa gagcatcaag ttcaccccca gcaaccacaa gctgctcgag gccctgacca
     2101 cccggtccaa gaaaacctac gaccagtgga aggacagctt cgagttcacc cagaacaagc
     2161 tgatcaccac ccgcgagagc ctggaaaacg tgaagcagtg catcaccggc ggcagcatgc
     2221 acaagtgccg gaagcacttc gagacagtgt tcgacctgct gaacctgttc gacatcttca
     2281 gccggggcac caacctgtgc aactacgcca gcatctgcca gatcaacctg cggagaaaag
     2341 tgacaaagtt cctgcacttc accgagatcc agctgagata cgacgccgcc aacaaggata
     2401 tccagaaaat ggccgagttc gtgaaccagc tgaagagaga gagccacagc gccctggccg
     2461 ccgtggtcac caggaccaag aagtccgcct acggcgtcgt gggccacgtg aacaacgcca
     2521 tcaagaacgc cgccgtgaac gacatcaaga atagcgccgc catcggcgcc gtgaaagtca
     2581 ccaatagcgc ccacaacaag cctaccggca acgtgtacac cgacaccagc aacgccctgt
     2641 ttgacaagct ggaaaagatg gacaaggaca gcgtgaacct gagaagcctg tctggcgcgc
     2701 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     2761 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2821 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2881 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2941 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     3001 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     3061 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     3121 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     3181 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     3241 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     3301 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     3361 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     3421 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3481 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     3541 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     3601 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     3661 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     3721 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     3781 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3841 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3901 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3961 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     4021 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     4081 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     4141 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     4201 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     4261 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     4321 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     4381 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     4441 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     4501 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     4561 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     4621 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     4681 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     4741 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     4801 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4861 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4921 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4981 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     5041 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     5101 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     5161 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     5221 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5281 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     5341 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     5401 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5461 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5521 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     5581 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     5641 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     5701 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5761 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5821 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5881 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5941 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6001 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6061 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6121 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     6181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6421 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7921 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7981 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     8041 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     8101 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     8161 gctcatgtcc aatatgaccg c
//
