LOCUS       sequence-213193-       10983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g00437 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g00437-bio-His
FEATURES             Location/Qualifiers
     source          1..10983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6147..6164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6216..6271
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6270..6289)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8507..8525)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8593..8697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8698..8766,8767..9558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8916..8935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9729..10317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10218..10237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10471..10488
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10605..10626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10641..10646,10647..10664,10665..10671)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10679..10695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10684..10706
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10703..10719
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10703..10719
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgcatgattg gctgctggtc accggcttcg accccgtgac catccagttc gacgtgctcg
     1261 tgaagcccat cgtgcaggcc acaatcgcca gaggcaccaa gtgcaccgcc gacggctgca
     1321 ccatcatcac caacctgccc aagatcgtgc tgatcaacgt ggccaccaac agcttcaccc
     1381 tgcggctggt ggacacccac aatgtggcca gcgccgatgg ctaccaggtc aactgcgcct
     1441 tcctgatcgc ctggattgtg ctggaaagcg gctacttcca cgagagcacc cacagcgaca
     1501 tcctgtacgc cggcagcatc gacctggact tcgccaagcc caccttcagc gtgaccctgg
     1561 acaacagcaa gctgaagctg acccccggca acagcgcctt tagcgccatc ctggtggcca
     1621 aggacgacga ggacatcagc gacctggtca acatctactt cagcagccag atctacaccc
     1681 agccccacga tctggacacc gtgtccatca gctttaccag caacgccgcc accaagctga
     1741 gcaagaccag caagacagcc aagctgcact acttcctcgt gaacacctat aagctgcaga
     1801 aaaccagcat cggccgggac aagaacacag ccgacttcta cgtgcgcaag atcttcctga
     1861 aggacggcat gaccatgccc ctggaatggg cctaccccga gcccctgctg ctgccctgcc
     1921 agagcatcac cgaccccacc gacaaccctg tgtccaccat caccgccaag atggacttcc
     1981 agtggatcgc ccggaccaac gaggccaaga ttttcgagtt ctacatcatc cagaagtccc
     2041 cagccctgag cggcgacgag ctgctgtcca agaatatcta ctttctgatc ctgaacatgg
     2101 ccaagcctcc ccctctgctg aaactggata tcctgcctct gaatatgggc ttctacgaca
     2161 ccaagaacgt gtacaagagc ttcagcgccc ctcaggtggt gtacacctgg ctgtccggca
     2221 tcggcaacaa gtactgcctg ctggcccacg ccatcgagcc caacagcgac gtggactgcc
     2281 acgccctgtg caatttctac gccagcaacc ccaagtattg ccacagatac ggcaccggca
     2341 tggaaatcga taccgtggaa acctacaacc gcttcgccac aaacaatatg gccgactgca
     2401 tcgagaagtt cctcatccct ctgtgcaagt tccccaacgt gcgggacctg tccgacgaca
     2461 acatgatgga atccgagtac cctctcgagg accagcagcc caccctgctg aagaatgtgg
     2521 aaccctccgc cttcgcctgc agaggctctg ccccaggcgg cgactacgtc gtgtgccgga
     2581 tgagccagct gaaggataag tggttctaca gcagctatca agtctgcaag tactacctcg
     2641 agcacaaggg cgagcactgg accagctccg tggtgtctat cgccgccagc gacgacgaca
     2701 catctgccgg cggaggcgcc aaatattacg tgacctacga gatctgcaag gtggtggtca
     2761 atgccgccaa ttttctgaag tgccacgagc tgggcaacaa aaactgccct aagctgcccg
     2821 acctgcagtt ctacccaatc gagaaccaga agatgcacag caactacatc ctctacagct
     2881 tttgctgccc caaccgggcc agcaccaaga gcaacatcaa gccctccaac gacgccatcc
     2941 ccgacggcgt gatcctgtct gatgagcggc atgcccaggg cctggccagc atgcccgaca
     3001 tctaccactc ccccaaggac aaggataaca ccggggccaa actgcacgac agcatcatga
     3061 acgacgtgaa gcagtcccag atccagaaca acgccattct gagcgccaag caccagacct
     3121 ccatctccat cgacatctcc aacaccctgt ctggcggcat tctgtacgtg cccatctacg
     3181 gcagcgaggt ggcccccacc tacatcaaag agacatgcat ctacagcgag tgggaagagt
     3241 ggtccgcctg cgagctgcct tgtcagctga aaaacaaggt cgtgaatcgg cggagaaggc
     3301 ggacactgct gcagggcatc gggaccaaat gccacaccat cgaaaccgag ccttgccagg
     3361 acatccctga ctgcagcagc atgtgcctga tgtccgagtg gtctgaatgg tccaaatgca
     3421 aggtcgacaa agagaacggc ctgagctaca gcgtgcggca tatcctgtat gactccgaca
     3481 agtgcaagga ccagacactg gaactgtggc ggagctgcaa ggatgctgac ctggatgagt
     3541 acaccaccaa ggactcctac gacctgaatc tggccatcaa tgagctggaa ttcgccggga
     3601 acatggacaa agaatacgac atcacaaacg actacaagat ggccgtgtcc tccacttgga
     3661 gcggctgtac ccagccttgc gacaaccgga acaaccgggg cgtgaacatt gccctgaaag
     3721 acagcgagcg gagcaccaac accatcaata ccagatcctg cacagagaag tttcccccct
     3781 gtgccttcga cgaagtgatc aattgcgaag agatccggcc cgtgctgcct ggcctgacca
     3841 agcagagctg catcagcggc tgccggacca tcctgagcaa ctgcaagacc gaccacctga
     3901 agtgttacgc ctttgccgaa ctgggcagca agcaggataa gaacttcttc aacaactgcc
     3961 ggctcaacga cgaactcgag aagaagatcg ctcaggtcaa aaacctccag tacaacaagt
     4021 gctacatcag cagggccagc tacctggtgg atgagaacag ctggatcgac gcccggtaca
     4081 acgcctgcaa ctgctacgaa gagggcgccg tgccctgtga cgagcaggac gtgttcctga
     4141 gcctggaaga tctgcccgag gatatgatgg cctccagcgt gtgccccgtg gaagccctgg
     4201 ccccctccga gatgaatacc gccgatatgg tgtccttcgg ccacaacaag gccgcctact
     4261 gccccctcga gtacatgcgg aagggcaccg tgaccttcgg ctctatcagc tacgaaaccc
     4321 tgagaaactt ctgcgagtac ggcgctggct acatgaacct gaagaacagc cccagataca
     4381 gcctgttcga gtaccagtgc ccctacgcca ccaccctggg caatagactg gaccccgccg
     4441 tgacaaccag cgactgtatc gccgagtgta acaagtatta caccgagtgc aagaacgaga
     4501 tcaccacact gagcacatgc ctgatccaca gcctgcggag cctgtccaaa gagttcaaca
     4561 agtacaagtg tcacctgagc gagatctacc ccctggactg cgacatggcc tacagctcct
     4621 ccgccgagct gagcgacgat agccacatct gtcccgccat ctgtaagtat aacaagcggc
     4681 gctgcgaagt gaagatcttt gagaacgatt acaacaacta caagcggtgt ctgaaagaga
     4741 agctcaagac aagcgagatg tttaacaaca attgcatgtt caagcccatg ggccggatgc
     4801 ccggcagcgg cttcgtgctg tgtaaattcc cacccgtgga ttgcaagtat agcgaatggt
     4861 ctgagtggag caagtgtaca gccccctgca accggatcga taatgtggct acccggaagc
     4921 ggatgcggac cctgattcac cccgcctcca actacggcaa gccttgcaat agctacgaca
     4981 gcctgggcac cattgacatc gaaatctgca aacacctccc accatgccag tctgacaagt
     5041 ggatcctgcc agtgacagtg gaaagcaacc ctctgattct ggatttcgtg gacaatcggg
     5101 acgatgagca gaaccacgag atcgagtggg tcaaagaggc cttcgaagtc taccccaaac
     5161 tgaagcacag cagcgaagtc aagtcctgca agctgtacaa gtccgagaaa accgagggct
     5221 gcgggtgccc cagaggctac agcccttgtt ccctgggcga gtccatcaag agccccgatc
     5281 tgatcacctc tatcaagaac ctgtgcagcg tgaacccact ggcctttatc agcttcgagg
     5341 acgatgtgga cgtgtactac tacctgtgta acgtggacat ggtgtatgcc gaggatgaac
     5401 tcgacatgga tatcaagaga tgcgactcta tcgacagctt ccagtactcc agctgcatgg
     5461 gcaacggaaa gctgtttaag cccttcaact tcatcggcgc gccgtcgacc tccatcacgg
     5521 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     5581 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     5641 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     5701 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     5761 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     5821 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     5881 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     5941 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     6001 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     6061 gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg gacaggaacc
     6121 tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc tagacacgtg
     6181 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     6241 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     6301 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     6361 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     6421 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     6481 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     6541 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     6601 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     6661 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     6721 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     6781 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     6841 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     6901 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     6961 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     7021 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     7081 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     7141 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     7201 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     7261 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     7321 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     7381 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     7441 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     7501 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     7561 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     7621 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     7681 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     7741 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     7801 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     7861 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     7921 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     7981 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     8041 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     8101 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     8161 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     8221 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     8281 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     8341 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     8401 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     8461 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     8521 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8581 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8641 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8701 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8761 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8821 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8881 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8941 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9001 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9061 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9121 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9181 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9241 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9301 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9361 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9421 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9481 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9541 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9601 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9661 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9721 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9781 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9841 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9901 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9961 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10021 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10081 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10141 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10201 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10261 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10321 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10381 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10441 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    10501 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    10561 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    10621 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    10681 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
    10741 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
    10801 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
    10861 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
    10921 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
    10981 cgc
//
