LOCUS       sequence-213200-        5003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ser-tRNA synthetase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Villarreal F, Contreras-Llano LE, Chavez M, Ding Y, Fan J, Pan T, 
            Tan C
  TITLE     Synthetic microbial consortia enable rapid assembly of pure 
            translation machinery.
  JOURNAL   Nat Chem Biol. 2018 Jan;14(1):29-35. doi: 10.1038/nchembio.2514. 
            Epub 2017 Nov 13.
  PUBMED    29131146
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2514"; journalName: "Nat Chem Biol"; date: 
            "2018-01"; volume: "14"; issue: "1"; pages: "29-35"
COMMENT     Sequence Label: B6-3
FEATURES             Location/Qualifiers
     source          1..5003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..141
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     175..194
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        175..193
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             233..255
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1555..1572
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1646..1664)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1660..1707
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2316..3266)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3314..3536
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4028..4122)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4153..4947)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4264..4283)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4874..4893
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
       61 gcgccccagc tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata
      121 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga tgatcccgcg aaattaatac
      181 gactcactat aggggaattg agagcggata acaattcccc tctagaaata attttgttta
      241 actttaagaa ggagatatac catgcatctc gatcccaatc tgctgcgtaa tgagccagac
      301 gcagtcgctg aaaaactggc acgccggggc tttaagctgg atgtagataa gctgggcgct
      361 cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac
      421 tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt
      481 ctggaagtga acaaactggg cgaagagctg gatgcagcaa aagccgagct ggatgcttta
      541 caggctgaaa ttcgcgatat cgcgctgacc atccctaacc tgcctgcaga tgaagtgccg
      601 gtaggtaaag acgaaaatga caacgttgaa gtcagccgct ggggtacccc gcgtgagttt
      661 gactttgaag ttcgtgacca cgtgacgctg ggtgaaatgc actctggcct cgactttgca
      721 gctgcagtta agctgactgg ttcccgcttt gtggtaatga aagggcagat tgctcgcatg
      781 caccgcgcac tgtcgcagtt tatgctggat ctgcataccg aacagcatgg ctacagtgag
      841 aactatgttc cgtacctggt taaccaggac acgctgtacg gtacgggtca actgccgaaa
      901 tttgctggcg atctgttcca tactcgtccg ctggaagaag aagcagacac cagtaactat
      961 gcgctgatcc caacggcaga agttccgctg actaacctgg tgcgcggtga aatcatcgat
     1021 gaagatgatc tgccaattaa gatgaccgcc cacaccccat gcttccgttc tgaagccggt
     1081 tcatatggtc gtgacacccg tggtctgatc cgtatgcacc agttcgacaa agttgaaatg
     1141 gtgcagatcg tgcgcccaga agactcaatg gcggcgctgg aagagatgac tggtcatgca
     1201 gaaaaagtcc tgcagttgct gggcctgccg taccgtaaaa tcatcctttg cactggcgac
     1261 atgggctttg gcgcttgcaa aacttacgac ctggaagtat ggatcccggc acagaacacc
     1321 taccgtgaga tctcttcctg ctccaacgtt tgggatttcc aggcacgtcg tatgcaggca
     1381 cgttgccgca gcaagtcgga caagaaaacc cgtctggttc ataccctgaa cggttctggt
     1441 ctggctgttg gtcgtacgct ggttgcagta atggaaaact atcagcaggc tgatggtcgt
     1501 attgaagtac cagaagttct gcgtccgtat atgaacggac tggaatatat tggccatcat
     1561 catcaccatc attaaagcag aggccatatg ctcgaggatc cggctgctaa caaagcccga
     1621 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     1681 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca
     1741 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gcctagggta
     1801 cgggttttgc tgcccgcaaa cgggctgttc tggtgttgct agtttgttat cagaatcgca
     1861 gatccggctt cagccggttt gccggctgaa agcgctattt cttccagaat tgccatgatt
     1921 ttttccccac gggaggcgtc actggctccc gtgttgtcgg cagctttgat tcgataagca
     1981 gcatcgcctg tttcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt
     2041 gttcaatttc atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta
     2101 catgctgttc atctgttaca ttgtcgatct gttcatggtg aacagctttg aatgcaccaa
     2161 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
     2221 acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg
     2281 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
     2341 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
     2401 tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
     2461 gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg
     2521 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
     2581 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
     2641 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     2701 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     2761 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     2821 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     2881 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     2941 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     3001 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     3061 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
     3121 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     3181 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     3241 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     3301 taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt
     3361 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     3421 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     3481 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     3541 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     3601 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     3661 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     3721 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     3781 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     3841 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     3901 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     3961 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtccctag
     4021 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     4081 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctctcgaacc
     4141 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc
     4201 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc
     4261 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc
     4321 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc
     4381 gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag
     4441 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc
     4501 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt
     4561 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc
     4621 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc
     4681 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag
     4741 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt
     4801 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc
     4861 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc
     4921 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
     4981 tcttgatccc ctgcgccatc aga
//
