LOCUS       sequence-21321-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCAG-mir30
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           23..116
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(339..358)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    404..459
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(458..477)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 actagggaaa cagggtaatt gtttgaatga ggcttcagta ctttacagaa tcgttgcctg
       61 cacatcttgg aaacacttgc tgggattact tcttcaggtt aacccaacag aaggctcgag
      121 caaccagaat tcaaggggct actttaggag caattatctt gtttactaaa actgaatacc
      181 ttgctatctc tttgatacat ttttacaaag ctgaattaaa atggtataaa ttatcacttt
      241 tgatatcatc gatgcggccg cactcctcag gtgcaggctg cctatcagaa ggtggtggct
      301 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
      361 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
      421 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
      481 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
      541 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
      601 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
      661 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
      721 gccagatttt tcctcctctc ctgacta
//
