LOCUS       sequence-213228-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for highly efficient expression of engineered IL24 with 
            mutated binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Zahradnik J, Kolarova L, Peleg Y, Kolenko P, Svidenska S, Charnavets
            T, Unger T, Sussman JL, Schneider B
  TITLE     Flexible regions govern promiscuous binding of IL-24 to receptors 
            IL-20R1 and IL-22R1.
  JOURNAL   FEBS J. 2019 Oct;286(19):3858-3873. doi: 10.1111/febs.14945. Epub 
            2019 Jun 12.
  PUBMED    31152679
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.14945"; journalName: "FEBS J"; date: "2019-10"; 
            volume: "286"; issue: "19"; pages: "3858-3873"
COMMENT     Sequence Label: pSumo24P7
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             860..877
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aactttaaga aggagatata ccatgagcaa gcatcaccat cattcaggcc atcaccatac
       61 cggacaccac catcattcag gcagtcatca ccattccgga tctgctgcgg gtggcgaaga
      121 agataagaaa ccggcaggtg gcgaaggtgg cggtgcccat atcaacctga aagtgaaagg
      181 tcaagacggc aacgaagtct ttttccgcat caaacgttct acccagctga aaaagctgat
      241 gaacgcatac tgtgaccgtc agtctgtaga catgaccgca attgctttcc tgtttgatgg
      301 tcgtcgcctg cgtgcggaac agaccccgga tgaactggag atggaagatg gcgacgaaat
      361 cgacgcaatg cttcatcaga ctggtggcgc gttccatttt ggtccatgtc gtgtcgaagg
      421 tgtggtgccg caagaactgt gggaggcctt ctgggcggtt cgtgatacgt tacaagctca
      481 ggacaacatc accgatgtac gtttacttcg tgcagaggtt ttacaaaatg tttcagatgc
      541 agagagctgc tatttagtcc atcaactgct gcgcttctac ttagacacag tctttaaaaa
      601 ctatcacaat aagaccgcag agcttcgtac tctgaaaagt ttttcaacac tggcgaataa
      661 tttcttacag atccgcaagg atctgcaacc gtgtcaggaa caaaatatgt gctcatcgcg
      721 tgaggaagca caccgtcgct ttttacaatt ccaacgtgca tttgagcaat tggatgtgga
      781 agcagcagcg acaaaggctc tgggagagat cgacatcctt cttcgctgga tgcaaaagtt
      841 ttatcaactg taactcgagc accaccacca ccaccactga gatccggctg
//
