LOCUS       sequence-213288-       11557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low Sensor Backbone: Used to make miRNA sensors (BbsI cloning) . 
            Plasmid is inherently unstable; please see Depositor Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB
FEATURES             Location/Qualifiers
     source          1..11557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    1196..1220
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1231..1240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1237..1929
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    complement(1952..1976)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2067..2088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2103..2108,2109..2126,2127..2133)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2141..2157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2165..2181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2177..2500
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    2194..2250
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2251..2268)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2251..2267)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2260..2282)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2476..2495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     polyA_signal    2763..2818
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    3202..3227
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    3899..3919
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3968..5146
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4199..5137
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    5163..5187
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5192..5201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5198..5200,5201..5203,5204..5917)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(5243..5264)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(5919..5943)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6098..6153
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6514..6530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6538..6554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6562..6568,6569..6586,6587..6592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6607..6628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6842..6861)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6853..7662
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6920..6939)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(7914..8502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7994..8013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8673..9464,9465..9533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9296..9315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9534..9605)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    9622..9659
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             9690..10289
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9690..9709)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10186..10206
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10455..10510
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    11489..11509
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgtgg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag aagggcgaat tcgacccaag
     1201 tttgtacaaa aaagcaggct ggatccaggt gccaccatgg tgagcgagct gattaaggag
     1261 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     1321 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggcggtcgag
     1381 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
     1441 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccccgagggc
     1501 ttcacatggg agagagtcac cacatacgaa gatgggggcg tgctgaccgc tacccaggac
     1561 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1621 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagacactg
     1681 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
     1741 ggccacctga tctgcaacct taagaccaca tacagatcca agaaacccgc taagaacctc
     1801 aagatgcccg gcgtctacta tgtggacagg agactggaaa gaatcaagga ggccgacaaa
     1861 gagacatacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1921 ctggggcaca aacttaattg agaattctac cacccagctt tcttgtacaa agtggtacgt
     1981 ctcaagacca gaggtctctg cttaagtctt cgcagctggc acgacaggtt tgccgactgg
     2041 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     2101 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     2161 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct
     2221 agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
     2281 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     2341 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2401 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2461 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2521 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2581 agctgtgacg aagacttcaa ccagaagaga cctgtgcttc actcctcagg tgcaggctgc
     2641 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     2701 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     2761 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     2821 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     2881 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     2941 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     3001 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     3061 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     3121 tgtccctctt ctcttatgga gatcccgagc ggtgcgtttt tatgcttgta gtattgtata
     3181 atgtttttaa gatccaagct tctgacctct tctcttcctc ccacagggcc tcgagacaat
     3241 tgcatcgatg gtaccagtga aagtctgcaa tgaattcgag ttggcgcgcc tgtcattcta
     3301 aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa
     3361 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     3421 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     3481 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     3541 agggggctgt ccccgtgagc tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct
     3601 aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt
     3661 cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg
     3721 ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc
     3781 cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc tgtccccgtg
     3841 agctcccagg cgcgccaact cggtaccgta tcgatgtcga cgttaacgct agtgatatca
     3901 actttgtata gaaaagttgg ctccgaattc gcccttgaca gcagagatcc agtttggtta
     3961 attaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4021 gttgtgggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     4081 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     4141 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     4201 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     4261 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     4321 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     4381 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     4441 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     4501 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     4561 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     4621 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     4681 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     4741 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     4801 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     4861 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     4921 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     4981 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     5041 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     5101 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagaag ggcgaattcg
     5161 acccaagttt gtacaaaaaa gcaggctgac cgccaccatg gtgagcaagg gcgaggagct
     5221 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5281 cagcgtgagg ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     5341 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgagccacgg
     5401 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     5461 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcacctacaa
     5521 gacccgcgcc gaggtgaagt tcgagggcga caccctagtg aaccgcatcg agctgaaggg
     5581 cgtcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     5641 ccacaacatc tatatcatgg ccgtcaagca gaagaacggc atcaaggtga acttcaagat
     5701 ccgccacaac gtggaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     5761 catcggcgac ggccccgtgc tgctgcccga cagccactac ctgagcaccc agtccgtgct
     5821 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcc gcaccgccgc
     5881 cgggatcact ctcggcatgg acgagctgta caagtaatac ccagctttct tgtacaaagt
     5941 ggtacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     6001 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     6061 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     6121 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     6181 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     6241 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     6301 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     6361 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     6421 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     6481 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6541 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6601 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6661 gggaaacctg tcgtgccagc ggatcgacag tactaagctt ggtctaatac ccaatctctc
     6721 gtcttatcca gatgttttat acgcctctgg cccgtgtctc aaaatctctg atgttacatt
     6781 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     6841 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     6901 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     6961 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     7021 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     7081 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     7141 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     7201 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     7261 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     7321 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     7381 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     7441 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     7501 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     7561 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     7621 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     7681 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     7741 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag aggtatcact agtattacag
     7801 aggtaagtta taacagtcgc ctaacccaat tgaggggata acgcaggaaa gaacatgtga
     7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8341 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaacca
     8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9001 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9061 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9541 ctttttcaat attattgaag catttatcaa ggttattgtc tcatgagcgg atacatattt
     9601 gaatgtaccg aatttctcga gggcttgtcg acgacggcgg tctccgtcgt caggatcatg
     9661 aattcgaccg cctgtctcaa ggtgccacca tgaccgagta caagcccacg gtgcgcctcg
     9721 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     9781 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     9841 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     9901 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     9961 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
    10021 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
    10081 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
    10141 ccgagcgcgc cggggtgccc gccttcctgg agacatccgc gccccgcaac ctccccttct
    10201 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
    10261 ggtgcatgac ccgcaagccc ggtgcctgag ctttgagact acgcgtgaat tcactcctca
    10321 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
    10381 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
    10441 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
    10501 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
    10561 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
    10621 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
    10681 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
    10741 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
    10801 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttga
    10861 attctctaga attaccctgt tatccctatg cttggtttac cgagctctta ttggttttca
    10921 aacttcattg actgtgccca acgcgcctgg gagctcacgg ggacagcccc cccccaaagc
    10981 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
    11041 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
    11101 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
    11161 tgcagacacc tggggggata cggggaaaaa gctaggctga aagaccggtt cttagaatga
    11221 cacgcgcctg ggagctcacg gggacagccc ccccccaaag cccccaggga tgtaattacg
    11281 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
    11341 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
    11401 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
    11461 acggggaaaa agctaacttc tgagacgaca actttgtata gaaaagttgg ctccgaattc
    11521 gcccttgaca gcagagatcc agtttggtta attaatt
//
