LOCUS       sequence-213296-       12627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to make miRNA sensors with targets in 5' and 3/ UTRs (with BsaI
            cloning) . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 12627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSBr5and3
FEATURES             Location/Qualifiers
     source          1..12627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        336..547
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          548..1486
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    1564..1585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1600..1605,1606..1623,1624..1630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1638..1654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1662..1678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1674..1997
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    1691..1747
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1748..1765)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1748..1764)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1757..1779)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1973..1992)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     regulatory      2110..2119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2116..2808
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     RBS             2923..2934
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2942..3652
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3087..3105)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    3887..3942
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    4334..4359
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    5031..5051
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5100..6278
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5331..6269
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    6295..6319
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6324..6333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6330..6332,6333..6335,6336..7049)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6375..6396)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(7051..7075)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7230..7285
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7646..7662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7670..7686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7694..7700,7701..7718,7719..7724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7739..7760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7913..8017
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8239..9030
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      complement(9306..9894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9386..9405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10065..10856,10857..10925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10688..10707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10926..10997)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    11014..11051
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             11082..11681
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11082..11101)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11578..11598
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    11847..11902
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 gcgcctggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc
       61 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
      121 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
      181 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
      241 gggaaaaagc tttaggctga aagagagatt tagaatgaca ggcgcgcctg gtggcgaacg
      301 atcaactact ttaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      361 ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
      421 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      481 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      541 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
      601 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
      661 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
      721 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
      781 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
      841 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
      901 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
      961 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     1021 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     1081 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     1141 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     1201 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     1261 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     1321 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     1381 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     1441 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgg aagacaacag
     1501 atgagaccgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1561 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1621 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1681 tgattacgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct
     1741 cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1801 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1861 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     1921 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     1981 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     2041 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacggtc tcaaggtttg
     2101 tcttcaggtg ccaccatggt gagcgagctg attaaggaga acatgcacat gaagctgtac
     2161 atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc
     2221 tacgagggca cccagaccat gagaatcaag gcggtcgagg gcggccctct ccccttcgcc
     2281 ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa ccacacccag
     2341 ggcatccccg acttctttaa gcagtccttc cccgagggct tcacatggga gagagtcacc
     2401 acatacgaag atgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     2461 ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag
     2521 aagaaaacac tcggctggga ggcctccacc gagacactgt accccgctga cggcggcctg
     2581 gaaggcagag ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacctt
     2641 aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat
     2701 gtggacagga gactggaaag aatcaaggag gccgacaaag agacatacgt cgagcagcac
     2761 gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaattga
     2821 gaagacaagc tttgagaccg aattcaagct tggagcgttg acaccatcga atggtgcaaa
     2881 acctttcgcg gtatggcatg atagcgcccg gtacttctag agaaagagga gaaatactag
     2941 aatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     3001 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3061 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     3121 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     3181 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     3241 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccttgcagga
     3301 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     3361 aatgcagaag aaaaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     3421 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     3481 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     3541 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     3601 acgcgccgag ggccgccact ccaccggcgg catggacgag ctttacaagt aagcttctcg
     3661 gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc ttttttcgtt ttggtcccgc
     3721 tggtctcaca acttgtcttc attcactcct caggtgcagg ctgcctatca gaaggtggtg
     3781 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     3841 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     3901 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     3961 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     4021 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     4081 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     4141 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     4201 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     4261 tggagatccc tcgacctgcg ctggtgcgtt tttatgcttg tagtattgta taatgttttt
     4321 aagatccaag cttctgacct cttctcttcc tcccacaggg cctcgagaca attgcatcga
     4381 tggtaccagt gaaagtctgc aatgaattcg agttggcgcg cctgtcattc taaatctctc
     4441 tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     4501 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     4561 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     4621 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggagggggct
     4681 gtccccgtga gctcccaggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt
     4741 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     4801 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     4861 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     4921 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccccg tgagctccca
     4981 ggcgcgccaa ctcggtaccg tatcgatgtc gacgttaacg ctagtgatat caactttgta
     5041 tagaaaagtt ggctccgaat tcgcccttga cagcagagat ccagtttggt taattaattg
     5101 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttgtggg
     5161 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     5221 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     5281 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     5341 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     5401 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     5461 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     5521 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     5581 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     5641 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     5701 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     5761 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     5821 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     5881 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     5941 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     6001 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     6061 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     6121 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     6181 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     6241 tggttcaaag tttttttctt ccatttcagg tgtcgtgaga agggcgaatt cgacccaagt
     6301 ttgtacaaaa aagcaggctg accgccacca tggtgagcaa gggcgaggag ctgttcaccg
     6361 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga
     6421 ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca
     6481 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagt
     6541 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6601 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg
     6661 ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat cgagctgaag ggcgtcgact
     6721 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca
     6781 tctatatcat ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     6841 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     6901 acggccccgt gctgctgccc gacagccact acctgagcac ccagtccgtg ctgagcaaag
     6961 accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca
     7021 ctctcggcat ggacgagctg tacaagtaat acccagcttt cttgtacaaa gtggtacgcg
     7081 tgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     7141 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     7201 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     7261 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     7321 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     7381 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     7441 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     7501 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     7561 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     7621 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7681 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7741 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7801 tgtcgtgcca gcggatcgac agtactaagc ttggtctaat acccaatctc tcgtcttatc
     7861 cagatgtttt atacgccctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7921 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7981 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
     8041 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     8101 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     8161 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     8221 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     8281 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     8341 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
     8401 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     8461 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     8521 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     8581 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     8641 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     8701 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     8761 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     8821 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     8881 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     8941 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     9001 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     9061 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaggtatca
     9181 ctagtattac agaggtaagt tataacagtc gcctaaccca attgagggga taacgcagga
     9241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10201 ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10921 ctcatactct tcctttttca atattattga agcatttatc aaggttattg tctcatgagc
    10981 ggatacatat ttgaatgtac cgaatttctc gagggcttgt cgacgacggc ggtctccgtc
    11041 gtcaggatca tgaattcgac cgcctgtctc aaggtgccac catgaccgag tacaagccca
    11101 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
    11161 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
    11221 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    11281 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    11341 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    11401 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    11461 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    11521 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacatcc gcgccccgca
    11581 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    11641 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agctttgaga ctacgcgtga
    11701 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
    11761 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
    11821 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
    11881 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
    11941 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
    12001 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
    12061 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
    12121 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
    12181 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
    12241 gcccaagctt gaattctcta gaattaccct gttatcccta ggtttaccga gctcttattg
    12301 gttttcaaac ttcattgact gtgccggagc ctcgagacaa ttgattaaca tcgatacggt
    12361 accgagttgg cgcgcctggg agctcacggg gacagccccc ccccaaagcc cccagggatg
    12421 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
    12481 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
    12541 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
    12601 ggggggatac ggggaaaaag ctttagg
//
