LOCUS       sequence-21330-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T
  TITLE     Impaired microRNA processing enhances cellular transformation and 
            tumorigenesis.
  JOURNAL   Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003.
  PUBMED    17401365
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2007-05"; volume: "39"; issue: "5"; pages: 
            "673-7"
COMMENT     Sequence Label: pSicoR human Drosha2
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        108..607
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(143..166)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     540..559
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(651..670)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gaagctcttt ggtgaataat tcaagagatt attcaccaaa gagcttcttt tttctcgagt
       61 actaggatcc attaggcggc cgaattcgtc gacctcgaaa ttctaccggg taggggaggc
      121 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      181 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      241 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      301 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      361 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
      421 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
      481 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
      541 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
      601 cctttcgacc tgcatccatc tagatctcga tcgagcagct gaagcttacc atgaccgagt
      661 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
      721 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
      781 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
      841 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
      901 cggggggcgg tgttcgccga gatcgggccg cgcatggccg a
//
