LOCUS       sequence-21331-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T
  TITLE     Impaired microRNA processing enhances cellular transformation and 
            tumorigenesis.
  JOURNAL   Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003.
  PUBMED    17401365
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2007-05"; volume: "39"; issue: "5"; pages: 
            "673-7"
COMMENT     Sequence Label: pSicoR human Drosha3
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        125..624
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(160..183)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     557..576
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(668..687)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gagaaaagcc ttgttttgaa ttcggcagcc caaatattca agagatattt gggctgccga
       61 attctttttt ctcgagtact aggatccatt aggcggccga attcgtcgac ctcgaaattc
      121 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
      181 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
      241 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
      301 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
      361 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
      421 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
      481 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
      541 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
      601 tcttcctcat ctccgggcct ttcgacctgc atccatctag atctcgatcg agcagctgaa
      661 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg ac
//
