LOCUS       sequence-213314-        8812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shGAC, silence glutaminase GAC isoform, doxycycline inducible, 
            puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8812)
  TITLE     Dias lab diverse plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: tet-pLKO.puro_shGAC
FEATURES             Location/Qualifiers
     source          1..8812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..1918
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     1835..1854
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    1872..1890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1900..1918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2015..2132
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2181..2691
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2610..2628
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          2700..3272
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3280..3299
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3327..3345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3356..3976
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3429..3448)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4011..4584
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4178..4195)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4405..4424
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4604..5203
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4604..4623)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5100..5120
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5331..5564
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5636..5757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5673..5692)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5727..5746
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5797..5932
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5953..5971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5981..5998)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5981..5997)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5990..6012)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6139..6594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6226..6245)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6436..6457
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6620..6724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6725..6793,6794..7585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6943..6962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7756..8344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8245..8264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8498..8515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8632..8653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8668..8673,8674..8691,8692..8698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8706..8722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8711..8733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8730..8746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8730..8746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8765..8785
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8767..8785
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggcc gcaatatttg catgtcgcta tgtgttctgg gaaatcacca
     1861 taaacgtgaa atccctatca gtgatagaga cttataagtt ccctatcagt gatagagaca
     1921 ccggcctctg ttctgtcaga gttctcgaga actctgacag aacagaggtt tttgaattct
     1981 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2041 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2101 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2161 cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     2221 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     2281 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     2341 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     2401 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     2461 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     2521 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     2581 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     2641 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatctg
     2701 tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg
     2761 gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg
     2821 gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc
     2881 ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa
     2941 tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt
     3001 ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta
     3061 taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc
     3121 ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta tggttacaat
     3181 gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta
     3241 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     3301 tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa ttgatatgtc
     3361 tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat
     3421 cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta
     3481 ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag
     3541 gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa
     3601 cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg
     3661 tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca
     3721 acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt
     3781 aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac
     3841 tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc
     3901 agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa
     3961 atgtgaaagt gggtccgcgt acagcggctc ccgggagttc tagggatctg cccctctccc
     4021 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     4081 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     4141 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     4201 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     4261 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     4321 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     4381 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     4441 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     4501 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     4561 ttcctttgaa aaacacgatg ataaggatcc accggagctt accatgaccg agtacaagcc
     4621 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     4681 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     4741 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4801 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4861 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4921 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4981 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5041 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5101 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5161 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     5221 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gtacctttaa gaccaatgac
     5281 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5341 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5401 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5461 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5521 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5581 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     5641 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5701 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5761 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5821 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5881 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     5941 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     6001 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6061 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6121 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6181 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6241 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6301 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6361 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6421 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6481 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6541 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     6601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6841 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6901 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6961 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7021 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7081 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7141 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7201 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7261 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7321 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7381 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7441 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7501 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7561 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7621 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7681 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7741 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7801 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7861 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7921 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7981 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8041 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8101 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8161 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8221 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8281 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8341 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8401 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8461 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8521 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8581 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     8641 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8701 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     8761 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aa
//
