LOCUS       sequence-21332-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T
  TITLE     Impaired microRNA processing enhances cellular transformation and 
            tumorigenesis.
  JOURNAL   Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003.
  PUBMED    17401365
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2007-05"; volume: "39"; issue: "5"; pages: 
            "673-7"
COMMENT     Sequence Label: pSicoR human DGCR8-1
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        115..614
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(150..173)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     547..566
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(658..677)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cttgtttggg gtgagagtgc tgataattca agagattatc agcactctca cccctttttt
       61 ctcgagtact aggatccatt aggcggccga attcgtcgac ctcgaaattc taccgggtag
      121 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      181 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      241 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      301 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      361 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      421 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
      481 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
      541 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
      601 ctccgggcct ttcgacctgc atccatctag atctcgatcg agcagctgaa gcttaccatg
      661 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
      721 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
      781 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
      841 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
      901 gtcgaagcgg ggggcggtgt tcgccgagat cggcccgcgc atggcc
//
