LOCUS       sequence-213322-        3727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Vmn2r78
FEATURES             Location/Qualifiers
     source          1..3727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..37
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18..36
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(886..904)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(887..904)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(922..938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(922..938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(935..957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    946..962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(970..976,977..994,995..1000))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1015..1036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1153..1170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1324..1912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1404..1423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2083..2874,2875..2943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2706..2725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2944..3048)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3126..3581)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3263..3284)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3475..3494
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3707..3727,1..2)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3721..3727,1..11)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(3722..3727,1..11)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgacggccag tgaattgtaa tacgactcac tatagggcga attgggcccg acgtcgcatg
       61 ctcccggccg ccatggcggc cgcgggaatt cgattgaaag ttgtgacctt tctggattac
      121 aaagaaccct tgggaatggc tctggctgtc ttggctgtgt tcttttcttc tcttacattt
      181 attatactct gtgtcttctt gaaacaccga gacactccca tagtcaaggc caataatgaa
      241 actctcagct atgtcctact cacctccctc atattttgtt ttatttgttc cttgctctac
      301 attggttatc ccactatggt tagctgcatc ctgcaacaga ccacatttgc ccttgtgttc
      361 actgtggctg cctcttgtgt cttagccaag acaattactg tagtcctagc atttaagatc
      421 actgttccag gaagaagact gaggtggctg ctactttcag gggcacctaa ttacatcatt
      481 cccatatgca ccacaataca aatgattctc tgtggaatct ggctgggaac ctctcctcca
      541 tttgttgatg ctgatctaca catgatacat gaacacatca tcattgtttg caacaaaggt
      601 tcagtgactg ccttctactg tgtcctggga tacatgggct ctcttgctct tgcaagtctc
      661 actgtggctt tcctagccag gaatctccct gacacgttca atgaagccaa gctcctgaca
      721 ttcagcatgc tggtgttctg cagtgtctgg gtcaccttcc tccctgtcta tcatagcact
      781 aagggcaagg ctatggttgc tgtggaatca ctagtgaatt cgcggccgcc tgcaggtcga
      841 ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct
      901 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      961 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1021 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1081 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1141 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1681 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1741 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1801 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1861 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1921 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1981 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2041 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2101 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2161 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2221 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2281 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2341 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2401 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2461 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2521 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2581 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2641 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2701 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2761 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2821 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2881 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2941 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3001 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3061 aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     3121 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     3601 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3661 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3721 tgtaaaa
//
