LOCUS       sequence-213325-       12055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU68.1-2.2
FEATURES             Location/Qualifiers
     source          1..12055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1095
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(1342..1358)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1342..1358)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1869..1892
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6949..6960)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(7844..7864)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7844..7862)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8201..8789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8281..8300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8960..9751,9752..9820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9583..9602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9821..9925)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9952..11294
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10915..10962)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11335..11353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11648..12055
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca
       61 gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat
      121 ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc acttccagat
      181 gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt gggtggtcct
      241 aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt
      301 caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct
      361 ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgtcagaga attagtggga
      421 ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa
      481 caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa
      541 catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga
      601 ttatggagaa aaactgtgga ggaaaccatc aagaacgaat tccctacatt gaaggttcaa
      661 catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt
      721 attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca
      781 ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca
      841 tttggtttgt acgaaccatg ccacggttct gctccagatt tgccaaagaa taaggtcaac
      901 cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa
      961 gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt
     1021 gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag
     1081 aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa ctttataaat
     1141 gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga
     1201 aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa
     1261 tacaggtaag caaattgata ctaatggctc aacgaaatga cggtatcgat aagcttgata
     1321 tcgaattcct gcagcccggg ggatccacta gttctagatt aattaatatc taaaaattgc
     1381 cttatgatcc gtctctccgg ttacagcctg tgtaactgat taatcctgcc tttctaatca
     1441 ccattctaat gttttaatta agggattttg tcttcattaa cggctttcgc tcataaaaat
     1501 gttatgacgt tttgcccgca ggcgggaaac catccacttc acgagactga tctcctctgc
     1561 cggaacaccg ggcatctcca acttataagt tggagaaata agagaatttc agattgagag
     1621 aatgaaaaaa aaaaaaaaaa aaaggcagag gagagcatag aaatggggtt cactttttgg
     1681 taaagctata gcatgcctat cacatataaa tagagtgcca gtagcgactt ttttcacact
     1741 cgaaatactc ttactactgc tctcttgttg tttttatcac ttcttgtttc ttcttggtaa
     1801 atagaatatc aagctacaaa aagcatacaa tcaactatca actattaact atatcgtaat
     1861 acacaatgga ttataaggat gatgatgata agactagtat gactatctac accccaacat
     1921 ctggtggaga gctcgacatg ggcttgccag gagtgaaact catatatttc ggcaacgagt
     1981 ttcccaaaga tgacttggaa gacctttttc gccgcataca caaccacagt aaagacaata
     2041 atcactttat actaactcaa ttcataaacg aggctacatt ggcaatcaag gacgagatcc
     2101 ggcgcctacc gactgaaatg aaacaaagta tacccccttt tgacaccatt ttgagatggg
     2161 ccgagcctaa cgagcttcga aatggcttga tgtgtggtgc tgtcgatggt gttcttctgg
     2221 tagtcgctca gctagcggcg tatatcggat atattgagaa ttcttccagg gagtttaacg
     2281 acagtacaaa cttatgtctt gctggccttg gaattggctt actagcatcg accgcaattt
     2341 ctttgtccaa aacgctcgtc gacatcccac ttgctggcgc agatgtcgtt cgtttggcgt
     2401 ttcgtctggg gattcatgtc atgggcgtat ccgagaatct agaagctcgt gatttgtcag
     2461 agacaccaag tacatgggct tacgtggtgc ataacgccga ttcagatgat gtccagagtg
     2521 aactagattt tatgcactct cgttcagaaa ctccggacac tggtaagatt tttatcagtg
     2581 ctgtaagccg cacttctgtg acagtgagtg ggccaccaac aaggttgaat gctattttca
     2641 acaaatcgca attctttcga gaaaagagat tcattgccct gcctgtttat ggtggattat
     2701 gccatgctcc tcacgtttac ggagtggcag atactcacag cattgtccac gggacccatt
     2761 tgaaaacggt cagggcaaac ttttcaccaa cgattccagt ctactccacc agcactggtc
     2821 tgccataccc attcaagact gccgcggaca tttttgaaag cgtcgtgatg gagctcctca
     2881 caaaggccat ttattgggac cgtgtcattc atggtttggt agaccgaatg aaggaaaatg
     2941 cgacatccga agccgttcta ttcagctttg gcaattccat acctctcaac gacctccaga
     3001 ctgcaatcaa aaatgatgtg ccctccgtcg aactctcaac caagagtctg atctcatggc
     3061 ctttccattt gaatcataac gactttacac ctcgcacccc agcccaagct aagctggcca
     3121 tcattggcat gtcttgtcga cttccgggag gtgctacaaa cacagaaaag ttttgggggc
     3181 ttttagaaaa tggccttgat gtttctcgaa gaataccagc tgataggttc gatattgaga
     3241 cacactttga tccaacgggc aaggacctaa acaagaccat gactcaatac ggctgcttca
     3301 ttgaagaacc tggcttgttt gacgcccctt ttttcaatat gtcccctcgt gaagcacagg
     3361 ttgtagatcc gcagatgcgc ctttctttgg tcactgcgta tgaggctctg gagcgcgctg
     3421 gctatgttgg taaccgcacc gcttctacgc ggttggaacg tattggcaca tactatggcc
     3481 aagctgccga tgattaccgc gaagtcaacc aaggtcagga ggtcagtact tactatattc
     3541 caggtggctg tcgagccttt gggcctggcc ggataaatta tttcttcaaa tttgcaggtc
     3601 ccagctacag tattgatact gcttgctcct ccggtcttgc ggcgattgag gttgcctgtc
     3661 aggctatttg gagtggtgct gttgatatgg ctgttgcagg gggtgtcaat gtgctaacca
     3721 accctgatgg ctttactgga ttatgcaaag gccacttcct tactcaggga cacaacgcgt
     3781 gcaaaacttg ggatgcaacc gccgacggat attgcagagc tgacggtgta ggctcggtcg
     3841 taatcaagaa actcgaggat gcagaggcag ataacgataa catcttggga gttattctgg
     3901 gtgcgggaac aaaccattct gcggaagcag tctccattac tcacccccat gcaggccatc
     3961 aagcctacct atctcgccag gtcctcggaa aatgtggagt gaatcctttg gatgtgtcat
     4021 atatcgaact tcatgggact ggcactcaag ctggtgatca tgaagagatg cagggaatca
     4081 tggaagtata tgcaccactg accaaacgcc gcaataaaga ccagcctctg catatcgggt
     4141 ctgttaaagc gaacattgga catggggaat ctgttgcagg cgtttcatca ttgatcaaag
     4201 tactgctcat gctccaaaag aatgccatcc cgccccatat tggtatcaaa acgaacatca
     4261 accccaagtt taaagatctc gataagcgca acttgcatat accttttcag aagacccctt
     4321 ggcccacagt tccaggtaag agacgacttt tagcactcaa caattttggc gccgctggtg
     4381 gcaacacgac gatggtccta gaagaagccc cggatcgtgt tattctagag aaagaccatc
     4441 gtccaaacca tgttattaca gtttcagcca aaactaaagg atctttgacc gggaatatag
     4501 agcgccttgt tgcctatctt gatggacatc cagatacaaa catcgcggat ctggcataca
     4561 caacgaatgc gcgtcgctat caccacagcc atcgtgtagc aatagccaca tctgatatag
     4621 cacatctcac aaaacagctc aaatctcgtt tagagaaggt cgatttggta aatcctgtta
     4681 aaagctccga accgccacca gttgcatttg tgttcactgg tcagggcgct tcttacaaat
     4741 caatgagctt ggaattgtat cgcgatatcc cgacatttca acagcacatc caacatcttg
     4801 actcactggc tcgtggacaa gggtttccct catttattcc ggcacttgat ggctcgtttc
     4861 ccaaagacca tgcgcattcc ccagtcgtta cacacttggc attggcttgc acggagatag
     4921 ccatggccaa atattggatt tctctagggg ttaagccaga tgttgttctg ggccacagtc
     4981 tgggtgaata tgcggcgatg cacatagctg gtgtagtcac tgcaagtgat accatattta
     5041 tggtgggacg cagggcacaa atgctggagg agaggtgcaa aatcggcagt catgctatga
     5101 tggcggtacg agcctccctg tctcaaatag acgaagtctg tgctggcaag tcatataagt
     5161 acactattgc ctgtgtcaac agtccttttg atacagtcct tagcggtacc aatcatcaaa
     5221 ttgatgacct agcgtcggtg ctcgagggtg tcggttaccg ctgtattaag ctggacgtgg
     5281 cttttgcatt tcactccgaa cagactgatc ctattttgga cgattttgaa gctatttcaa
     5341 agaccggcgt tgtcttctca gagccaaaag tacccgtaat atcgccatta cttggaaaag
     5401 ttgtttttga cggcaaaact ttcagcgcaa attacgtccg gcgggcaact cgagagacgg
     5461 tagatttttc atcggcgtta gagagtgctc ggatgatttc tacgattagc gatgacacta
     5521 tttggattga aattgggcct cacccggtct gtgttggctt tgtcaagtct acaatcccat
     5581 ctacccaact cacagttgca tcgatgcgcc gtggtgagga caattggaaa accatggcgg
     5641 aagccatggc ttctttgcac gttgccagcg tggaagttgg ttggaatgaa tttcatcgtc
     5701 cgttcgagag caaactccgg cttcttgatc tgccgactta tgcttggaac gaaaagaact
     5761 actggttgca gtataacggt gactggtgct tgacaaaggg caataccttc tacaccaagg
     5821 agcagtcgac ccaggccaaa tcaaccatca agtccctacc acgaagtgaa atccagacaa
     5881 catctgttca gagaattatt gagcagacgt tcgaggggcc aactggtacg gtggttatgc
     5941 agtcagacct tatgcagact gatctactcg ctgctgctca tggacatcga atgaacaact
     6001 gcggcgtcgt aacatcctca atccacgccg atatcgcata cactcttggt gattacctat
     6061 accgcaagct agtttccaaa cccgagcaag ccaatatgaa tattgccaat cttgtcgtga
     6121 caaagggcct tgtggctcaa agcaataacg aagtgtctca actgattcga gtgactgctg
     6181 ttacgtctaa tgttcacgcc ggcaaggtgg aattgacatg gcaaaatgtt gacaatgacg
     6241 gaaacgttca cgagccgttt gctactgcag aacttcatta tgaaagagca tccgaatgga
     6301 tttcttcttg gactcccgtc catcatcttg tgcaaggccg aatcgaggcc cttgagcgct
     6361 tggccgtcga aggcaaagcc aaccgctttt cgcgcagtat ggcatatact ttattcgcca
     6421 acaatttggt tgattatgaa gacaaatatc gaggcatgca atcagttgtc atgtacgagc
     6481 tggaggcttt cgctgatgtg caacttacca caaaagagcg aggggtttgg gttattcctc
     6541 cctattttat tgatagttta gcacatttag gcgggtttgt tatgaactgt tctgacgcga
     6601 tggacacgaa agacaatttt tccgttactc cgggttggaa atccatgcaa tttttgaagc
     6661 cgttgcttcc aggtgccaaa tatcgttcgt atgtcaagat gattccgaca atcgaggacc
     6721 caaccatctt cctaggcgac gtttatatct tgcaagacaa ctccatcgtc gggatggtgg
     6781 gtggcctaca gtttcggcgt tatccacgtc tgctgctcaa tcgattcttc tcgccgccgg
     6841 atagaatacc agccgtgggt gatgaaccaa caccgcctgc accaaaggct tcagtatcaa
     6901 caccagcaca tcaaacccaa aagtcaaaac cacggtcaaa gtcagtttct gcagttccca
     6961 ctccagactc cagcaatgcc gtgcagagcc aacgcgatac tccgttgcag gaagctgcta
     7021 ccactggcaa tactggccct gagccagcat cgtctgctga tactgcatct gcttcaggca
     7081 gtatcactgc caaagcgctg atgcttattg cccgggaagc agcgttggat atctcagact
     7141 tggaggatga tgccagtttc tcgcaccttg gtattgacag tttgatgtca ctcgtcatag
     7201 cagagaagtt tatgggggaa ctaggtgtca aagtcggtgg cagtctgttc ctggactacc
     7261 cgaccattgg tgatttgcga aaatggctgg acgaatacta tgcttgagga gattgataag
     7321 acttttctag ttgcatatct tttatattta aatcttatct attagttaat tttttgtaat
     7381 ttatccttat atatagtctg gttattctaa aatatcattt cagtatctaa aaattcccct
     7441 cttttttcag ttatatctta acaggcgaca gtccaaatgt tgatttatcc cagtccgatt
     7501 catcagggtt gtgaagcatt ttgtcaatgg tcgaaatcac atcagtaata gtgcctctta
     7561 cttgcctcat agaatttctt tctcttaacg tcaccgtttg gtcttttata gtttcgaaat
     7621 ctatggtgat accaaatggt gttcccaatt catcgttacg ggcgtatttt ttaccaattg
     7681 aagtattgga atcgtcaatt ttaaagtata tctctctttt acgtaaagcc tgcgagatcc
     7741 tcttaagtat agcggggaag ccatcgttat tcgatattgt cgtaacaaat actttgatcg
     7801 gcgctatgcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     7861 ttgcgcgctt ggcgagactc aaacaagata tattttgccc gccttacaga cgaaactagt
     7921 tggaggttat ggagcatact atcacgtggg cggccactgg tgagttacta caccccaggg
     7981 gcaacgttga tgctcctaaa aaactctggc tggacgcaag ccgtaacacc cgtgtcactt
     8041 cataatcgtt tgcaattcag ggcttgatct acacattacc ctgttatccc tattaattaa
     8101 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8161 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8221 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8281 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8341 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8401 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8461 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8521 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8581 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8641 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8701 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8761 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8821 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8881 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8941 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9001 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9061 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca
     9961 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
    10021 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
    10081 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
    10141 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
    10201 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
    10261 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
    10321 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
    10381 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
    10441 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
    10501 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
    10561 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
    10621 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
    10681 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
    10741 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
    10801 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
    10861 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
    10921 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
    10981 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
    11041 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
    11101 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
    11161 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
    11221 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
    11281 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
    11341 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgagcg cgtttcggtg
    11401 atgacggtga aaacctcggc gcgccattac cctgttatcc ctaatataca acgcaaagaa
    11461 gctggaaaat tggcgaggga atcctgtttc tgtctatcca agaatgggca tgaggtggca
    11521 accgtcgtgc tagcgtacag ggtgcacttt gtaaccattt gggacaccgg acactcgctg
    11581 ttttcgaaat taccctttaa gcgcgggtat tgaaccaggc ttatgcccaa gatcgtagca
    11641 agcatttaac tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca
    11701 accattattt ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa
    11761 attcgatgac tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc
    11821 aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg gaaccggctt ttcatataga
    11881 atagagaagc gttcatgact aaatgcttgc atcacaatac ttgaagttga caatattatt
    11941 taaggaccta ttgttttttc caataggtgg ttagcaatcg tcttactttc taacttttct
    12001 taccttttac atttcagcaa tatatatata tatatttcaa ggatatacca ttcta
//
