LOCUS       sequence-213328-       12061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU36.1-2.2
FEATURES             Location/Qualifiers
     source          1..12061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..534
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6492..6512)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6492..6510)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6849..7437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6929..6948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7608..8399,8400..8468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8231..8250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8469..8573)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8600..9942
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9563..9610)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9983..10001
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10296..10703
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10704..11798
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12045..12061)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12045..12061)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaggcag aggagagcat
      301 agaaatgggg ttcacttttt ggtaaagcta tagcatgcct atcacatata aatagagtgc
      361 cagtagcgac ttttttcaca ctcgaaatac tcttactact gctctcttgt tgtttttatc
      421 acttcttgtt tcttcttggt aaatagaata tcaagctaca aaaagcatac aatcaactat
      481 caactattaa ctatatcgta atacacaatg gattataagg atgatgatga taagactagt
      541 atggccgtgc ttaccccaac cacggattcg gaatccgacg aggagtctcg taagatggaa
      601 gttgtctatt ttaacaatga gttctcaaag gaggacctgc aggatgtttt ccgccgtcta
      661 cacaaccaca gcaaggacaa acattacccc gtcttgaccc agttcatcca cgaggcctct
      721 tgggttataa aggacgaagt tcggcaactg ccgactgaga ctaagcagct gattccgcca
      781 ttttctaccc tcctggcttg ggtggagaat acccagcttc gggaaggact actgtgcagc
      841 gccgtagatg gagtgttgct cgtctttgtc cagctcgcag cttatattgg atatgtcgag
      901 agccgctctg gagatttgcc tgattttcca actacatatc ttgctggtct gggcaatggc
      961 ctactatcat caagtgccgc ctcattggca cgaacattgg gagatctgcc attggcctgt
     1021 gccgatgcgg ttcgtctcgc cttccgacta ggcattcact ctcttgatat ttcagagaat
     1081 ttggaagccc gcgatttatc agaaagcccg gatccatggg cgtatattgt ccacgacata
     1141 gatccggctg cggcttataa ggagctagat gctatacatt cccgagacga gacacctaat
     1201 acaagcaaaa tatttgtcag tgctgtcagc cggaattctg tcacaattag cggaccacct
     1261 tcgaggttaa aaacgctgct taataagtcg gagtttttcc gtgagtcgaa agtcgtcgca
     1321 atgccagtat acggcggcct ctgccacgcg ccgcatatat tcggtctgga cgatgtacat
     1381 agtgtcgttc atggctgtgc attaaacgcc acggccacaa gttctccgcc ggcgatgccg
     1441 gttttatcga cgggtaccgg tttgccgtac ctggcaaaga attctgtgga gatgttcgaa
     1501 ggtgtcgtct cagaactatt cacgaaaaca atttgttggg ataatgttgt tagcggcata
     1561 gtagaacgag tcaagaccaa cagcaccgcg gaagtaatgc ttcactgttt tggtaattct
     1621 acccctctta atgatctcat agttgcattg aagagcaatg ttccaaatgt cgatgtctcg
     1681 accaataacc tcatgatgtg gatctcgcag gttgttccaa gagatgctac cccccgcggc
     1741 accgggcagt caaaactggc tattgtaggt atgtcatgca gacttccagg gggcgcgaca
     1801 aacaccgaga agttttggga gatcttggaa aatggccttg acgtctcgcg acgaatccca
     1861 gctgaccgct tcgacattga tacgcactac gaccccactg ggaagcagca gaacaagacc
     1921 atgacacagt acggctgctt tattgatgag cccggtttgt ttgacgcacc cttttttaac
     1981 atgtctcctc gtgagtctca ggtcgtcgat ccgcagatgc gattagctct tgtcactgca
     2041 tatgaagcac tggagcgtgc tgggtatgtc ggtaatcgta cggcttccac acaattacaa
     2101 cgcataggaa catattacgg acaagctgcg gatgattacc gtgaggtcaa ccaaggtcaa
     2161 gatgtcggca cgtattatat tccgggtggt tgccgtgcct tcggtcctgg tcgtatcaat
     2221 tactttttta agtttgcggg acctagctat agcattgaca cagcttgctc ctctgggctc
     2281 gcggcgattg aagttgcttg tcaggctctt tggcatggcg atattgatat ggccgtcact
     2341 ggcggtgtta atatattgac taacccggac gggttcaccg ggctatgcaa aggctatttc
     2401 ttaaccaagg gtcacaacgc ttgcaagaca tgggattcca ctgctgatgg gtattgtcgt
     2461 gctgacggaa tcgggtcgct tgttattaag agactcgaag acgctgaggc ggacaacgac
     2521 aacatcttgg gggtcatcct tggagcagga accaaccatt cggccgaggc cgtctctatt
     2581 acccaccccc acgctggcca tcaagcatat ttatcccgtc aggtgcttcg gcaggccggc
     2641 gtggatccat tagacgtgtc atatgttgag cttcatggta ccggtacaca agctggcgat
     2701 cacgaagaaa tgcagggcat aatggaggtg tatgctccac taataaggcg gcgccggaag
     2761 gatcagcctc tgcacattgg agctgtcaag gccaatattg ggcatggcga gtctgttgca
     2821 ggcacagcgg cattaatgaa ggtgcttctc atgctgcaga aaaattccat cccgcctcat
     2881 attggtatca aaacggagat gaatcccaga ttcccccgag actttgacaa acgcaatctc
     2941 catattgact tggagatgac accttggccc cgaattccgt ctaagaaacg actggcggtt
     3001 gtcaataact ttggtgccgc tggcgggaac acaacaatgg ttttggaaga ggcaccaact
     3061 cgtgtcctat cggaggcaga tccccgcagt actcatatca tcgccatttc agccaagact
     3121 aaagcatcgt tggcaggaaa tatggagcgt ttgcttgcct atcttgatga acacccagat
     3181 attaaccttg ccgatctttc ctatacgacg actgcgcgcc gtactcatca tacccatcgt
     3241 gtagcaatta caacttccga catgggtcac ctgaagaagc agttggctgt tcgcctaggg
     3301 aatgtcgatt cgataaagcc tgttgatagt tctggcccgc caccagttgc tttcaccttt
     3361 actggccagg gttcttccta caagtcgatg aatctcgagt tataccgtga cgtgccaaca
     3421 ttcagagagc acattaagca tctcgactct atcgctcaac gtcaaggctt tccatctatt
     3481 gttcccgctc tcgatggctc acatcccaaa gagcacgcgc actttcctgt cgtcactcaa
     3541 cttgccttgg tctgcaccga aattgctttg gcaaaatatt ggatttctct cgggatcaaa
     3601 ccagacgtcg tcatgggtca tagcttgggt gagtatgctg caatgcatat tgctggcgtg
     3661 gttactgcaa gcgataccat attcatggtt ggacgtcgag ctcagatgct ggaagaaaaa
     3721 tgcaatatta acagccatag catgatcgca gtccgggctt ctctacaaaa aatagcagaa
     3781 agcttgggag aaaagccata cacaattgct tgtgtcaatg ggccttcaga tactgtgctc
     3841 agcggcacca aggagaatat ggatggggtc gctgagttac tcgaggctgc cggttaccgc
     3901 tgctttaagc tggacgtcgc gtttgctttc cactctgagc agactgatcc tattttggat
     3961 gactttgagg ccatctcaaa gactggtgtt gcctttcgtg agccgaaact accagttatc
     4021 tcgccattac tcggaaaggt gatttttgac agcaagacgc taaacgcaaa ctacgtccgc
     4081 cgagccacca gggagcctgt cgactttttg tctgctttag aaaatgctca aaaaatctct
     4141 accattagtg atgaaactgt ctggatcgaa attggaccac atcctgtctg tactaacttt
     4201 gtcaaagcca ctatcccgtc cacgaaactt gccgtctcat cgatgcgccg tggagataat
     4261 aactggaaaa caatggctga gagcatggct tccatgcatc ttgctggatt cggggtcagc
     4321 tggaacgaat ttcaccgccc atttgaaaac aggctacgcc ttttagattt gccaacatac
     4381 gcttggaatg acaaaaccta ttgggtgcag tataatggcg actggtgcct aactaaggga
     4441 aatactttct acgacgctga gaaagaggct gccagagtaa aggctgcgac aaagacgcta
     4501 cctgcgaatg agctttacac gacaaccgtg cagcaaatca tagatgagag tttcaacggt
     4561 ttctctggca cagtggtgat tcagtctgat cttatgcaac cagacttact tgctgctgct
     4621 catggacaca gtatgaacaa ctgcggcgtt gttacttctt ctatccacgc cgacctttcc
     4681 tataccctcg gtgactatat ataccgcaag atatacccca aagccaagga cgtcagcatg
     4741 aatttttcgg acctggttgt cacaaaggga cttgtcgctc aaactaacaa acaaattccc
     4801 caattgttcc gagtaactgc tgtcgccgcc gatgtccgct caggtgcggt caatttgacg
     4861 tggcagaatg tgaataatga tggaactgcg gacgagccat ttgcaactgg taaaatacta
     4921 tacggagatt caactgagtg gatatcatct tgggccccat ttgctcacct catccatggt
     4981 cgcatcgaag cgcttgagcg cctagccgca gaaggaaagg ctaaccgctt ctcgcgcaat
     5041 atggcataca ctctctttgc cagtaaccta gtcgactacg cagagaagta ccgtggcatg
     5101 cagtctgtag tgatgcatga gttcgaaggg tttgctgacg tgcagctaac aacgaaagag
     5161 ggcggagtct ggacagcccc accgcacttc attgacagtg tggcgcactt ggctggtttt
     5221 attatgaact gctcagacgc catggatacg aagaataact actgtgttac tcctggttgg
     5281 aactcgatgc gatttgctaa acctctgctt ccaggagcca agtatcggtc gtatgtcaag
     5341 atgattccta cagtcgagga cccaacggtt tactttggca atgtgtacat catgcaagac
     5401 gatgccatca tgggttttgt cggtggtatt cagttccgca gatatccgcg catccttctc
     5461 agccgttttt tctctccgcc agataagatg gcctccttcg aagccaagcc aaagggacag
     5521 gtgacttctt caaaaactac ggccccagca ttggaatcaa caaaaactgt tgcggtagaa
     5581 tcaaagccgg tcctctctgg cggtgaatcc ggccttggca gcgacgaaaa gcaggggaac
     5641 gctcttcctt ccagctcctc cgcccccacg ccacttgctc ccgaggcctc ggcgccatct
     5701 atccctgatt ctgcggcagc ttccgatact acaacggcaa aggcaattac gctcattgcc
     5761 aacgaggctg gtctggatct tgttgacatg actgacgatg ccgttttcgc taatttaggc
     5821 gttgacagct tgatgtcgct ggtcattgct gaaaagctca gaacagagct agatgtcaag
     5881 gtcaccggta gccttttctt ggactatgag acaattggcg atttgcggca atggctggat
     5941 gattattata gttagggaga ttgataagac ttttctagtt gcatatcttt tatatttaaa
     6001 tcttatctat tagttaattt tttgtaattt atccttatat atagtctggt tattctaaaa
     6061 tatcatttca gtatctaaaa attcccctct tttttcagtt atatcttaac aggcgacagt
     6121 ccaaatgttg atttatccca gtccgattca tcagggttgt gaagcatttt gtcaatggtc
     6181 gaaatcacat cagtaatagt gcctcttact tgcctcatag aatttctttc tcttaacgtc
     6241 accgtttggt cttttatagt ttcgaaatct atggtgatac caaatggtgt tcccaattca
     6301 tcgttacggg cgtatttttt accaattgaa gtattggaat cgtcaatttt aaagtatatc
     6361 tctcttttac gtaaagcctg cgagatcctc ttaagtatag cggggaagcc atcgttattc
     6421 gatattgtcg taacaaatac tttgatcggc gctatgcggc cgccaccgcg gtggagctcc
     6481 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgagactcaa acaagatata
     6541 ttttgcccgc cttacagacg aaactagttg gaggttatgg agcatactat cacgtgggcg
     6601 gccactggtg agttactaca ccccaggggc aacgttgatg ctcctaaaaa actctggctg
     6661 gacgcaagcc gtaacacccg tgtcacttca taatcgtttg caattcaggg cttgatctac
     6721 acattaccct gttatcccta ttaattaata cggttatcca cagaatcagg ggataacgca
     6781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7261 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7921 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8221 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8581 ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     8641 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     8701 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     8761 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     8821 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     8881 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     8941 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     9001 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     9061 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     9121 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     9181 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     9241 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     9301 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     9361 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     9421 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     9481 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     9541 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
     9601 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
     9661 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
     9721 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
     9781 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
     9841 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
     9901 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag aaaccattat
     9961 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10021 cggtgatgac ggtgagcgcg tttcggtgat gacggtgaaa acctcggcgc gccattaccc
    10081 tgttatccct aatatacaac gcaaagaagc tggaaaattg gcgagggaat cctgtttctg
    10141 tctatccaag aatgggcatg aggtggcaac cgtcgtgcta gcgtacaggg tgcactttgt
    10201 aaccatttgg gacaccggac actcgctgtt ttcgaaatta ccctttaagc gcgggtattg
    10261 aaccaggctt atgcccaaga tcgtagcaag catttaactg tgggaatact caggtatcgt
    10321 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
    10381 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
    10441 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
    10501 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
    10561 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
    10621 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
    10681 tatttcaagg atataccatt ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg
    10741 accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg
    10801 ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg
    10861 ctacaggtgt tccacttcca gatgaggcgc tggaagcctc caagaaggct gatgccgttt
    10921 tgttaggtgc tgtgggtggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt
    10981 tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat
    11041 ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg
    11101 ttgttgtcag agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg
    11161 atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa
    11221 tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag
    11281 ctaatgtttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg
    11341 aattccctac attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta
    11401 agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct
    11461 ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct
    11521 ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag
    11581 atttgccaaa gaataaggtc aaccctatcg ccactatctt gtctgctgca atgatgttga
    11641 aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt
    11701 tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg
    11761 atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat
    11821 atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg
    11881 gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg
    11941 agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgaaa
    12001 tgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
    12061 a
//
