LOCUS       sequence-213337-       15113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tergeting vector for Vmn2r1 gene knockout mice (∆C1 strain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Akiyoshi S, Ishii T, Bai Z, Mombaerts P
  TITLE     Subpopulations of vomeronasal sensory neurons with coordinated 
            coexpression of type 2 vomeronasal receptor genes are differentially
            dependent on Vmn2r1.
  JOURNAL   Eur J Neurosci. 2018 Apr;47(7):887-900. doi: 10.1111/ejn.13875. Epub
            2018 Mar 25.
  PUBMED    29465786
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/ejn.13875";
            journalName: "Eur J Neurosci"; date: "2018-04"; volume: "47"; issue:
            "7"; pages: "887-900"
COMMENT     Sequence Label: Vmn2r1-5Hprt-loxP TV
FEATURES             Location/Qualifiers
     source          1..15113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2628..3127
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2663..2686)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3060..3079
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3593..3604
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4060..4093)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6262..7065
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6325..6344)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6935..6954
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7097..7114)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7103..7327
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12237..12257)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12237..12255)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12276..12292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12276..12292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12289..12311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12300..12316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12324..12330,12331..12348,12349..12354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12369..12390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12507..12524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12678..13266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12758..12777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13437..14228,14229..14297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14060..14079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14298..14402)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14428..14883)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14565..14586)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14777..14796
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15010..15032
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15024..15041
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15025..15041
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15051..15070
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15051..15069
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     15094..15110
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     15095..15111
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcatgtat gtaatagaaa acatatttct attagtatat ttttgtgtat tatatatata
       61 tatatgtata catatatata tacacatata cacacatatg catatattat acatacaata
      121 tgtatatatt atcttatgga ggatgtgtgt ggggtgtgtt ttttgtgcat gtgtgggcag
      181 gaccatgtac acatacatgt gtgcctgtgg tttaaaaggc aaaaattggc attggctgct
      241 ttccttgcct gtgccacatt ttataaatta tggcagtctt gcacctgaac taagagccaa
      301 tttgtctagg gtgaattgct ggtttgtctt tggtatgctg cttctgccta caggctgtct
      361 gacatgcatg ctttatttac atttctgtga gtgctgagga taaaaactct ggttctccta
      421 ctgtcctagt cagggtttct attcctgcac aaaatatcat gaccaaaaag caagttgggg
      481 aggaaagggt ttattcagct tacacttcca cactgctgtt catcaccaaa gggagttagg
      541 actggaactc aagcaggtca gaaagcagga gctgatgcag aagccatgga gggatgttct
      601 ttactggctt gcctcccctg gattgctcag tctgctctct tattgatcca agactaccag
      661 cccagggatg gcaccaccaa caaggggacc tccccccttg atcactaatt gataaaatgc
      721 cttacagctg gatctcgtgg agacatttcc ccaactgaag ctcctttctc tgcgataatt
      781 ccatcctgtg tcaagttgac acaaaactag ccagtacacc taatttcaga acaatagctt
      841 taaccactga agcatctccc cagtcccaca ctcttgagtt tgaataggaa agtatataaa
      901 ggggaaagtt aaaacccaca cacagtaact taataatact acatgagtac tgactgggac
      961 tataaatgag catggtatca tgaaaatgat tggtctgtta aagtagaatg acaaggggga
     1021 tgttatgttt cttctgattt gtatttggca gttttgtctt aattttaaga aggaaataca
     1081 taggtaaaag agttacaatt tactggtaaa ttctacaacc ttagaatata attccattgt
     1141 gctaacttta tgtacactgt gagattttct cttgtatttt taaaggttaa ttaaaaatta
     1201 atagtgaata tgtgttttcc tatatgcaaa ctatgtgggt cctgtgcata tagatgccag
     1261 ctaagccaga agagggtgtc agctcccttg gaactaaaac tgaaaactgt tgtaagacac
     1321 tcaatgtggg tactgggacc tttacccaag tcctctgcaa gagcactgtg tgtattctta
     1381 actactgacc catctctcca gctgcctatg tatatttttg ttcttaagaa taagacctaa
     1441 aaggctttgc attccataat gtgactaaac cataaataac tcaaaattgc cttattttgt
     1501 actttttgct tattctatgt cttaggatga caatgaaagc tgtattcact tccagtgtac
     1561 atcaattttt ggtaggtttc tattgctttt gtgagaaaaa ggttttggtg gcaaggtgca
     1621 agataaaagg gtctacactg atatcaagat ctaatatgtt ggctgggctg ttttctgaaa
     1681 atattatgaa ttttatactc caaggaaagg tgtttgaaat ggggatgaga gggcaactgg
     1741 agaggactga aagaacagta gattgatctt ctaagtgagg tttgtcttag aggtgagtct
     1801 agtaagaaag gaagcacatg gattagacgt gtggctcagc actagcatgg gggtgtggtg
     1861 aggttagaga tgagaaggga gagtggacaa aggggagggg agtggagggg gggaggagga
     1921 ggagagggmg agrgagaggg rgagggagag ggagagggag agggagaggg agagggagag
     1981 ggagaggatg gaggagggaa ggaagagagt ggagagattg gagggaagga ggaaggaaga
     2041 aaggaagtga ggaagaattt tttagaagcg actagagata taagcctgaa actatagagt
     2101 gtattctgag agtagtagtt ggtctgttat taaagtataa caaaaatggt atgtgtgaaa
     2161 cagttcatta aatatatacg ggccttacag tattttttct agggtatttt gttagtatcc
     2221 tactcatcaa tgaggtaatg agagagctgt cacctctttt gatactttat gatgaagcag
     2281 gatactgtcc cttacctttg aagaccatgg atttacatgt agtgagactc aattctaaac
     2341 aagaaggata gtttctacaa attaaaatga gttttaaact tagaatccac atttttatta
     2401 tttattgttt caaagcatat ggggattttc tgaagtttgt tctcaaaggg ttactcggct
     2461 cccagtccag atccacaaat tgcaagtgat ttcaaggccc ctgattgaga catgaaaatt
     2521 atgtagcaag tagtgacaag ccaagtccac tgggacacac acatttccac tcaatcaagg
     2581 cgcttcacta aaacgtgtgt gatttccaca ctagatcgaa ttctaccggg taggggaggc
     2641 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     2701 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     2761 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     2821 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     2881 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     2941 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     3001 cgggctcagg ggcgggctca ggggcggggc gggcgcccgr aggtcctccg grggcccggc
     3061 attctgcacg cttcaaaagc gcmcgtctgc cgcgctgttc tcctcttcct catctccggg
     3121 cctttcgacc tgcagggggg ggggggggtc ttgctgcgcc tccgcctcct cctctgctcc
     3181 gccaccggct tcctcctcct gagcagtcag cccgcgcgcc ggccggctcc gttatggcga
     3241 cccgcagccc tggcgtcgtg attagtgatg atgaaccagg ttatgacctt gatttatttt
     3301 gcatacctaa tcattatgct gaggatttgg aaagggtgtt tattccccat ggactaatta
     3361 tggacaggta agtaagatct taaaatgagg ttttttactt tttcttgtgt taatttcaaa
     3421 catcagcagc tgttctgagt acttgctatt tgaacataaa ctaggccaac ttattaaata
     3481 actgatgctt tctaaaatct tctttattaa aaataaaaga ggagggcctt actaattact
     3541 tagtatcagt tgtggtatag tgggactctg tagggaccag aacaaagtaa acattgaagg
     3601 gagatggaag aaggaactct agccagagtc ttgcatttct cagtcctaaa cagggtaatg
     3661 gactggggct gaatcacatg aaggcaaggt cagattttta ttattatgca catctagctt
     3721 gaaaattttc tgttaagtca attacagtga aaaaccttac ctggtattga atgcttgcat
     3781 tgtatgtctg gctattctgt gtttttattt taaaattata atatcaaaat atttgtgtta
     3841 taaaatattc taactatgga ggccataaac aagaagacta aagttctctc ctttcagcct
     3901 tctgtacaca tttcttctca agcactggct atgcatgtat actatatgca aaagtacata
     3961 tatacattta tattttaacg tatgagtata gttttaaatg ttattggaca cttttaatat
     4021 tagtgtgtct agagcttggg ctgcaggtcg agggacctaa taacttcgta tagcatacat
     4081 tatacgaagt tatattaagg gttccggatc cccgggcgag ctcgaattct agagctatct
     4141 aatatatttt aaaggttgca tagcattctg tcttatggag ataccataac tgatttaacc
     4201 agtccactat tgatagacac tattttgttc ttaccgactg tactagaaga aacattcttt
     4261 tacatgtttg gtacttgttc agctttattc aagtggaatt tctgggtcaa ggggaaagag
     4321 tttattgaat attttggtat tgccaaattt tcctctaaga agttgaatca ttttatactc
     4381 ctgatgttat atgagagtac ctttctcttc acaatttgtc tctttttttt ttttttttga
     4441 gacaaggtct ctgttgccca ggctggggtg cagtgcagca gaatgatcac agttcactgc
     4501 agtctcaacc tcctgggttc aagcgatcct tccacctcag cctcctgagt agctgggact
     4561 ataggtgtgc gccaccactc ccagctaata tttttatttt gtagaaacag ggttcgccat
     4621 gttacccagc ctcccaaagt gctgggatta caggcatgag ccactggccc agtttctaca
     4681 gtctctctta atattgtata ttatccaaga aatttcattt aatcagaacc tgccagtctg
     4741 ataggtgaaa atggtatctt gtttttattt gcatttaaaa aaaattatga tagtggtatg
     4801 cttggttttt ttgaaggtat caaatttttt accttatgaa acatgagggc aaaggatgtg
     4861 ttacgtggaa gatttaaaaa aaatttttaa tgcatttttt tgagacaagg tcttgctcta
     4921 ttgtccaggc tggagtgcag tggcacaatc acagttcact ccagcctcaa catcctgcac
     4981 taaagtgatt ttcccacctc acctctcaag tagctgggac tacaggtaca tgctaccatg
     5041 cctggctaat tttttttttt ttgcaggcat ggggtctcac tatattgccc aggttggtgt
     5101 ggaagtttaa tgactaagag gtgtttgtta taaagtttaa tgtatgaaac tttctattaa
     5161 attcctgatt ttatttctgt aggactgaac gtcttgctcg agatgtgatg aaggagatgg
     5221 gaggccatca cattgtagcc ctctgtgtgc tcaagggggg ctataaattc tttgctgacc
     5281 tgctggatta catcaaagca ctgaatagaa atagtgatag atccattcct atgactgtag
     5341 attttatcag actgaagagc tattgtaatg accagtcaac aggggacata aaagtaattg
     5401 gtggagatga tctctcaact ttaactggaa agaatgtctt gattgtggaa gatataattg
     5461 acactggcaa aacaatgcag actttgcttt ccttggtcag gcagtataat ccaaagatgg
     5521 tcaaggtcgc aagcttgggc tgaacatcga gcgccagggc tccgtaaagc tactagagca
     5581 caggcggtgc cccaacgtcc tggggcctct ccactaataa cggctacttc caattgattg
     5641 gacgcgccat cttgcctgcc ttatgcatat tcagcggtga actgaatatt catgaacgag
     5701 gcccgtcccg tccctccctc cttcccccca cccccggaac ccgctccgga ggacccgaag
     5761 ggccccgcct tcattaccga tgcgtaggac aaaccatttt cccgatgtgt gtggggggat
     5821 actaatgaga gactttagct gaaaatgagc ctgaactccg aagctgagta aaaatggcct
     5881 aactttatcc tccgttctgt aagtcctcgg tttgagtgca cgggaaaccc gaaaggagga
     5941 cgacaggacc aggaacattc tcctcctcct gtcgcgtcag aaagaacacc caaccaggga
     6001 gccggagccc tagcgtcaac aactccgccg cgcgcgctcc gtgtaggccg gtgcgggcgg
     6061 ccccgtagcg caagggaggg cgggaaagga aggggcggga cacaagggcg aatctataaa
     6121 gggcgtcact cagccagttc tctcctcaga agcgccgaga gcgcgaccgg gacggttgga
     6181 gaagaaggtg gctcccggaa gggggagaga caaactgccg taacctctgc cgttcaggat
     6241 catcgaattc ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt
     6301 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6361 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6421 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6481 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6541 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6601 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6661 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6721 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6781 ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat
     6841 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6901 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6961 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7021 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagggga tcaattctct
     7081 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7141 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7201 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     7261 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     7321 tctatggctt ctgaggcgga aagaaccagc tggggctcga gatccactag ttctagcctc
     7381 gaggctagag cggccagctt catggccatt actgttgtca gtgcacactg tatatttagc
     7441 agtttctgct agctcacatt tcaccaatca caactttcct ttgatatgtt tcatgctcca
     7501 tatttgagat gtccttgtgt gaagggtgct aatcatctgt acatcttcag aaaatacaaa
     7561 gtttcatgaa gaattctgcc cttaattcta tgactgtttc tcattcttac agtgaagcag
     7621 aggcaatgga ggaggaagat atatgtaagg ctttacttac aactagatta ctgtagagcc
     7681 aagtattatt taccactgga gtttttacct ctttaattcc aaacaactgg aaatgagaac
     7741 ttagagcaca tcgaaatgta acacattgat agtttaggaa agggattatt tcatttgttc
     7801 tacgccctgc attggaactt ctgaaaattt ctttcccagc aatgttagac tgtaaaggct
     7861 actacataga tttcaaaata tcatttttca cttccttaga aataatgtct tattacatta
     7921 tagttgaaga agtggagaga agtaaatata ttctatttaa gataattccc tagttaggat
     7981 tatttagaat ttgtttaatt attgaaatta ggatttgagt agctacatct tggtcttata
     8041 atgttaaaaa atacatttct ttaaaattag atctttatgt gtatttttca ccaatattca
     8101 tcaacatttc cttctatcta ttcaacccca gtgctttcaa gacacttatt attcttccat
     8161 gtaatttaac ttctccttcc aaaggtctat gtagtgttca gaacccagtc taactaagca
     8221 atattagttt aattttttaa tcataatctt taactcactg attcagaacc tcaactgggg
     8281 gtttgaagaa agtttgtttt gaaacaaata actttaatgc aatactaatt ttagctgttc
     8341 atgtgttaaa agtacatgaa acaggaagac tttatattat tttgaaaata tttctcaaat
     8401 agagaaaaat aaggaaatgg agtaaaggag tattttatgt gtcatttgat agtcataaaa
     8461 agaggtttag atattatgaa atattgtgag cctcataata aaaattaatc aatgaaggaa
     8521 aatttattcc aaatgctttt tttagtaatt attttgtgat gatttaaagt aacttcccca
     8581 ctaaccctta aaatacttca tatgtagcat taattataga gagtcttctt actgcccatt
     8641 taacttacaa ttgttttctt actgctggta taattctgat aggtcttcct taatatgtta
     8701 cttgaccttt tccccttact gcttttaata ttttttcttt gatttagtgc atttggtgtt
     8761 ttgattatta tgtgacaaaa ggaatttctt ttctggtcca gtctatttgg tgttctgtag
     8821 gcttcttgta tgtttatggg catctctttc tttaggttag ggaagttttc ttctataatt
     8881 ttgttgaaga tctttactgg ccctttaagt tgaaaatctt gactctctta tatacctatt
     8941 atccttaggt tttgtcttca cattgtgtct tggatttcct ggatgttttg ggttaggagc
     9001 tctttgcttt ttgcttttcc tttgactgtt gtgtcaatgt tttctatggt atcttcttca
     9061 ttaattttga gattctctct tctatctctt gtattctgtt gtgatgcttg cctctatggc
     9121 tcctgatctc tttcatatat atatatatcc agggttatta cctttgtgat ttctttattg
     9181 tttctatttc tacttttaga tcttggatgg ttttgttcat ttcctttgcc tgttcaattg
     9241 tgttttcctg taattcttta agggattttt gtgtttcctc tttaagagct tctagttgtt
     9301 tacctgtgtt ctcctgtatt tgtttaaggg agttatttat gtctttaaag ttctctatcg
     9361 ccatcatgag aactaatttt agatccaaat cttgcttttc cggtgtgatg gtttatccag
     9421 gacttgctta tgtggaaaaa atgggttctg atgatgccaa gtaaccttgg tttctgttga
     9481 ttatattctt atgcttgcct ccagccatct gattatctct agtgctactt ggcctcacta
     9541 tatctgactg gagcctgttg ttcctgggat cctggttgtg tcagaactcc tcagagttca
     9601 gctatttctg tgaacctgtg attctaggat tctgtgatcc tgtgatcctg agatcctggg
     9661 tatgtcattg ctcctggaag tcaagctgcc tcctagaccc tgagatcctg gtgtgaccaa
     9721 tctcttggga tcctgtggtt ttggacgtgt tagagtgcct gagagtggag cttcttctgg
     9781 gtgttctggg actggctgca gagtttgcgc ccaaggtctg ctcagggcat tggaccagac
     9841 caggaggaac tcgtgcgtct ggtcgggcgg agttcctggg tacctgggtc tcacgggttc
     9901 cagttacttc tagtgttggc gcggatgttg tgtcctcctc acttctggtc ctatgatcct
     9961 agaagtgtta gagtgcctgg gattggattt tactcttgag tgttgtgcat ctcgctgagg
    10021 agttcgagcc caagggctgc tcagggcacc ggctcagacg ggaaggactg cacataaatt
    10081 tttattattc tgccttcagt tatctatgtg taccatgtac atgcttggag tgtgtggagg
    10141 ccagaagatg atatcagctc ctctgaatct gtagttaggg atagttgtga acatctatgt
    10201 gggagctggg aataaaacct ggatcctctg caaaagcaaa tgttgatcca tctccagtgt
    10261 cttgttgttg gatatttaat aaaaaaaaaa aattactgaa cagaagacaa agttaaatat
    10321 ctatacaaga ttttaagaca taatgtcatt ttggggtact tgtcttagta agttaaaatg
    10381 tgctttaaca ttgaagagag aaaaaagaaa atagaattag ttgacaaata tagggggaat
    10441 tgaaaaaatt gtggcattga acttcaatat tgattttaat gacatcatgt ttctatttgt
    10501 atgtgagaat caataaatat ttcttaatat tgaaactaaa atctcagata tggttatgca
    10561 attacagttt tattatacat aattcttgta atgctttact gtattcctcc tttacgtgcc
    10621 tcagtgcatt ttaggatttt aaaaattaca attaatattc agaagaacat gggtcaatat
    10681 aaggagaaga atcatatagc cccaatgact tccagtgaat gggaaaaaca tgttcaaaga
    10741 ggctgttgta tatctgctgg tgaagaatat tctgactaga agaatgtttt tgtgacaaag
    10801 agataattgg ccacttaaga aaatcatagc agattatata tctatatcta tgtctatgtc
    10861 tatgtctatg tctatgtcta tgtctatgtc tatgtctatg tctatgtcta tgtctatgtc
    10921 tatatctatc tatatctata tctcatctat atggtaaaaa aatccaaaag ttgcttccca
    10981 agattactaa tcaatagtga atatgattca cttgaatatg ttaagattgt attgttattt
    11041 gtgaaaaatt gatgtgtata gacaactaag atacctgctg ctgcttctac aaaaagctgt
    11101 aatccctcat ttaactgaaa ctcctttcct gaaaagcccc accccaactg caatagccag
    11161 tgcctcacaa aaatttttcc tttccaggtt taatttccgg ggtttccgat ggatgaaaac
    11221 catgatccac accattaagg agattaatga gaggaaggac attttgccca accatactct
    11281 gggctaccag atctttgatt cctgctatac catctccaaa gcaatggagt catctttggt
    11341 gtttcttact gggcaggaag aattcaaacc caattttaga aatagcactg gatcaactct
    11401 agcagcactg gttggatcag ggggatcatc tttgtcagtt gctgcttcac gaattctagg
    11461 gttgtattat atgcctcagg tatgtcagaa tattatgctg agtaattatt tgtaaagaaa
    11521 tagaaatggg accaggagag atgtctaaga ggttaagagt acttgttctt gtagaggatt
    11581 tagtttcaat ccccagaact gatcgggtag ttcacaacca tctgaaactc caggtatggg
    11641 gatctgaaag ccccctcccc ccatttttga ctcttatagg cttccttatt catgcagtgc
    11701 cataaactta tgcaagcaca cacacaaaaa tggtggcaca cacattttat cccaccactc
    11761 aggaagcaga ggcacacaga tctgagtcag aggctagtct gctctaccga gctagttctc
    11821 agacaactag gcttatacaa agaaaccctg cctcaaaaaa ataaacaaaa atatttaaag
    11881 aaatacagtg tttagaaatt gttgaaaata agaaagacag aagaagttga tataaactat
    11941 aagatagcct agtattggtg gagtggtgtt cttagtgaca ctgcataagc aagcctagta
    12001 tgggtggggt gatattctta gtgacactgt ataagtaagc ctaggatgcc tgggatggtg
    12061 ttctttgtga cactgtatac ctaagatcca gccatgagat ctggggctta cttagaagtg
    12121 acttcagatt acttgattca gaacatcctt cttgcactga gttgtgatgc agtaattata
    12181 tacgtctcac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
    12241 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    12301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    12361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    12421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    12481 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    12541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    12601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    12661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    12721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    12781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    12841 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    12901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    12961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    13021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    13081 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    13141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    13201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    13261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    13321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    13381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    13441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    13501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    13561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    13621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    13681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    13741 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    13801 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    13861 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13921 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    13981 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    14041 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    14101 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    14161 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    14221 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    14281 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    14341 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    14401 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
    14461 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
    14521 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
    14581 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
    14641 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
    14701 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
    14761 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
    14821 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
    14881 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    14941 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    15001 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
    15061 cactataggg cgaattgggt accgggcccc ccctcgaggt cgacggtatc gat
//
