LOCUS       sequence-213352-       12720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to express Cpf1 from EFS promoter, as well as a 
            Cpf1 FlipArray from U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Chow RD, Kim HR, Chen S
  TITLE     Programmable sequential mutagenesis by inducible Cpf1 crRNA array 
            inversion.
  JOURNAL   Nat Commun. 2018 May 15;9(1):1903. doi: 10.1038/s41467-018-04158-z.
  PUBMED    29765043
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-15"; volume: "9(1):1903. doi"; pages: " 
            10.1038/s41467-018-04158-z"
COMMENT     Sequence Label: pLenti-LbCpf1-U6_FlipArray
FEATURES             Location/Qualifiers
     source          1..12720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2611..2644)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2680..2713
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2742..2990)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2800..2819)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2970..2990)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3041..3252
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3260..3269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3272..3292
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3317..6997
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             6998..7045
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7052..7132
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             7142..7198
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7199..7795
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7692..7712
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7811..8399
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7864..7884)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8282..8293)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8471..8704
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8730..8747)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8736..8960
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9006..9434
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9093..9112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9303..9324
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9443..9463)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9448..9777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9628..9763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9690..9709
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9825..9872
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9891..10265
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10395..10516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10432..10451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10486..10505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10565..10581)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10565..10581)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10578..10600)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10589..10605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10613..10619,10620..10637,10638..10643))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10658..10679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10796..10813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10967..11555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11047..11066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11726..12517,12518..12586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12349..12368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12587..12691)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattta attaaaaaaa taccgttcgt atagcataca ttatacgaag
     2641 ttatgagacg tccgtctcat ctacacttag tagaaattat accgttcgta taatgtatgc
     2701 tatacgaagt tatagcgcta tctacactta gtagaaatta cggtgtttcg tcctttccac
     2761 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
     2821 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
     2881 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca
     2941 gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc ttctcgagag
     3001 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     3061 acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg
     3121 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     3181 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     3241 gccagaacac aggtccggtg ccaccatggc cccaaagaag aagcggaagg tcggtatcca
     3301 cggagtccca gcagccagca agctggagaa gtttacaaac tgctactccc tgtctaagac
     3361 cctgaggttc aaggccatcc ctgtgggcaa gacccaggag aacatcgaca ataagcggct
     3421 gctggtggag gacgagaaga gagccgagga ttataagggc gtgaagaagc tgctggatcg
     3481 ctactatctg tcttttatca acgacgtgct gcacagcatc aagctgaaga atctgaacaa
     3541 ttacatcagc ctgttccgga agaaaaccag aaccgagaag gagaataagg agctggagaa
     3601 cctggagatc aatctgcgga aggagatcgc caaggccttc aagggcaacg agggctacaa
     3661 gtccctgttt aagaaggata tcatcgagac aatcctgcca gagttcctgg acgataagga
     3721 cgagatcgcc ctggtgaaca gcttcaatgg ctttaccaca gccttcaccg gcttctttga
     3781 taacagagag aatatgtttt ccgaggaggc caagagcaca tccatcgcct tcaggtgtat
     3841 caacgagaat ctgacccgct acatctctaa tatggacatc ttcgagaagg tggacgccat
     3901 ctttgataag cacgaggtgc aggagatcaa ggagaagatc ctgaacagcg actatgatgt
     3961 ggaggatttc tttgagggcg agttctttaa ctttgtgctg acacaggagg gcatcgacgt
     4021 gtataacgcc atcatcggcg gcttcgtgac cgagagcggc gagaagatca agggcctgaa
     4081 cgagtacatc aacctgtata atcagaaaac caagcagaag ctgcctaagt ttaagccact
     4141 gtataagcag gtgctgagcg atcgggagtc tctgagcttc tacggcgagg gctatacatc
     4201 cgatgaggag gtgctggagg tgtttagaaa caccctgaac aagaacagcg agatcttcag
     4261 ctccatcaag aagctggaga agctgttcaa gaattttgac gagtactcta gcgccggcat
     4321 ctttgtgaag aacggccccg ccatcagcac aatctccaag gatatcttcg gcgagtggaa
     4381 cgtgatccgg gacaagtgga atgccgagta tgacgatatc cacctgaaga agaaggccgt
     4441 ggtgaccgag aagtacgagg acgatcggag aaagtccttc aagaagatcg gctccttttc
     4501 tctggagcag ctgcaggagt acgccgacgc cgatctgtct gtggtggaga agctgaagga
     4561 gatcatcatc cagaaggtgg atgagatcta caaggtgtat ggctcctctg agaagctgtt
     4621 cgacgccgat tttgtgctgg agaagagcct gaagaagaac gacgccgtgg tggccatcat
     4681 gaaggacctg ctggattctg tgaagagctt cgagaattac atcaaggcct tctttggcga
     4741 gggcaaggag acaaacaggg acgagtcctt ctatggcgat tttgtgctgg cctacgacat
     4801 cctgctgaag gtggaccaca tctacgatgc catccgcaat tatgtgaccc agaagcccta
     4861 ctctaaggat aagttcaagc tgtattttca gaaccctcag ttcatgggcg gctgggacaa
     4921 ggataaggag acagactatc gggccaccat cctgagatac ggctccaagt actatctggc
     4981 catcatggat aagaagtacg ccaagtgcct gcagaagatc gacaaggacg atgtgaacgg
     5041 caattacgag aagatcaact ataagctgct gcccggccct aataagatgc tgccaaaggt
     5101 gttcttttct aagaagtgga tggcctacta taaccccagc gaggacatcc agaagatcta
     5161 caagaatggc acattcaaga agggcgatat gtttaacctg aatgactgtc acaagctgat
     5221 cgacttcttt aaggatagca tctcccggta tccaaagtgg tccaatgcct acgatttcaa
     5281 cttttctgag acagagaagt ataaggacat cgccggcttt tacagagagg tggaggagca
     5341 gggctataag gtgagcttcg agtctgccag caagaaggag gtggataagc tggtggagga
     5401 gggcaagctg tatatgttcc agatctataa caaggacttt tccgataagt ctcacggcac
     5461 acccaatctg cacaccatgt acttcaagct gctgtttgac gagaacaatc acggacagat
     5521 caggctgagc ggaggagcag agctgttcat gaggcgcgcc tccctgaaga aggaggagct
     5581 ggtggtgcac ccagccaact cccctatcgc caacaagaat ccagataatc ccaagaaaac
     5641 cacaaccctg tcctacgacg tgtataagga taagaggttt tctgaggacc agtacgagct
     5701 gcacatccca atcgccatca ataagtgccc caagaacatc ttcaagatca atacagaggt
     5761 gcgcgtgctg ctgaagcacg acgataaccc ctatgtgatc ggcatcgata ggggcgagcg
     5821 caatctgctg tatatcgtgg tggtggacgg caagggcaac atcgtggagc agtattccct
     5881 gaacgagatc atcaacaact tcaacggcat caggatcaag acagattacc actctctgct
     5941 ggacaagaag gagaaggaga ggttcgaggc ccgccagaac tggacctcca tcgagaatat
     6001 caaggagctg aaggccggct atatctctca ggtggtgcac aagatctgcg agctggtgga
     6061 gaagtacgat gccgtgatcg ccctggagga cctgaactct ggctttaaga atagccgcgt
     6121 gaaggtggag aagcaggtgt atcagaagtt cgagaagatg ctgatcgata agctgaacta
     6181 catggtggac aagaagtcta atccttgtgc aacaggcggc gccctgaagg gctatcagat
     6241 caccaataag ttcgagagct ttaagtccat gtctacccag aacggcttca tcttttacat
     6301 ccctgcctgg ctgacatcca agatcgatcc atctaccggc tttgtgaacc tgctgaaaac
     6361 caagtatacc agcatcgccg attccaagaa gttcatcagc tcctttgaca ggatcatgta
     6421 cgtgcccgag gaggatctgt tcgagtttgc cctggactat aagaacttct ctcgcacaga
     6481 cgccgattac atcaagaagt ggaagctgta ctcctacggc aaccggatca gaatcttccg
     6541 gaatcctaag aagaacaacg tgttcgactg ggaggaggtg tgcctgacca gcgcctataa
     6601 ggagctgttc aacaagtacg gcatcaatta tcagcagggc gatatcagag ccctgctgtg
     6661 cgagcagtcc gacaaggcct tctactctag ctttatggcc ctgatgagcc tgatgctgca
     6721 gatgcggaac agcatcacag gccgcaccga cgtggatttt ctgatcagcc ctgtgaagaa
     6781 ctccgacggc atcttctacg atagccggaa ctatgaggcc caggagaatg ccatcctgcc
     6841 aaagaacgcc gacgccaatg gcgcctataa catcgccaga aaggtgctgt gggccatcgg
     6901 ccagttcaag aaggccgagg acgagaagct ggataaggtg aagatcgcca tctctaacaa
     6961 ggagtggctg gagtacgccc agaccagcgt gaagcacaaa aggccggcgg ccacgaaaaa
     7021 ggccggccag gcaaaaaaga aaaagggatc ctacccatac gatgttccag attacgctta
     7081 tccctacgac gtgcctgatt atgcataccc atatgatgtc cccgactatg ccggatccgg
     7141 cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgac
     7201 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7261 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7321 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7381 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7441 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7501 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7561 gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7621 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7681 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7741 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg
     7801 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7861 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7921 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7981 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     8041 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     8101 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8161 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     8221 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8281 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8341 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     8401 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8461 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     8521 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8581 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8641 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8701 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8761 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8821 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8881 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8941 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     9001 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9061 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9421 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9601 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9781 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     9841 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     9901 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     9961 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
    10021 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
    10081 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
    10141 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
    10201 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
    10261 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
    10321 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
    10381 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10441 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10501 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    10561 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    10621 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    10681 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    10741 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    10801 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    10861 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    10921 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10981 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    11041 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    11101 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    11161 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    11221 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    11281 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    11341 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    11401 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    11461 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    11521 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    11581 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    11641 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    11701 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    11761 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    11821 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    11881 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    11941 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    12001 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    12061 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    12121 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    12181 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    12241 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    12301 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    12361 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    12421 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    12481 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    12541 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    12601 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    12661 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
//
