LOCUS       sequence-21336-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T
  TITLE     Impaired microRNA processing enhances cellular transformation and 
            tumorigenesis.
  JOURNAL   Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003.
  PUBMED    17401365
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2007-05"; volume: "39"; issue: "5"; pages: 
            "673-7"
COMMENT     Sequence Label: pSuper mouse DGCR8-2
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        57..271
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     192..211
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        356..855
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(391..414)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     788..807
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(876..895)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt gaagatcccc cgggctgcag gaattcgaac
       61 gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc
      121 cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca
      181 atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt
      241 tgggaatctt ataagttctg tatgagacca cagatccctc tcaatgtgac tggatattca
      301 agagatatcc agtcacattg agagtttttg gaactcgagg ttaacgaatt ctaccgggta
      361 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
      421 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
      481 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
      541 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
      601 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
      661 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
      721 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
      781 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
      841 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
      901 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc g
//
