LOCUS       sequence-213360-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector encoding the EL222 gene under constitutive promoter J23106..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Jayaraman P, Yeoh JW, Jayaraman S, Teh AY, Zhang J, Poh CL
  TITLE     Cell-Free Optogenetic Gene Expression System.
  JOURNAL   ACS Synth Biol. 2018 Apr 20;7(4):986-994. doi: 
            10.1021/acssynbio.7b00422. Epub 2018 Apr 4.
  PUBMED    29596741
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00422"; journalName: "ACS Synth Biol"; date: 
            "2018-04-20- 20"; volume: "7"; issue: "4"; pages: "986-994"
COMMENT     Sequence Label: pEBLindv2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             42..53
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      770..841
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      857..884
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcctaga gaaagaggag aaatactaga
       61 tgttggatat gggacaagat cggccgatcg atggaagtgg ggcacccggg gcagacgaca
      121 cacgcgttga ggtgcaaccg ccggcgcagt gggtcctcga cctgatcgag gccagcccga
      181 tcgcatcggt cgtgtccgat ccgcgtctcg ccgacaatcc gctgatcgcc atcaaccagg
      241 ccttcaccga cctgaccggc tattccgaag aagaatgcgt cggccgcaat tgccgattcc
      301 tggcaggttc cggcaccgag ccgtggctga ccgacaagat ccgccaaggc gtgcgcgagc
      361 acaagccggt gctggtcgag atcctgaact acaagaagga cggcacgccg ttccgcaatg
      421 ccgtgctcgt tgcaccgatc tacgatgacg acgacgagct tctctatttc ctcggcagcc
      481 aggtcgaagt cgacgacgac cagcccaaca tgggcatggc gcgccgcgaa cgcgccgcgg
      541 aaatgctcaa gacgctgtcg ccgcgccagc tcgaggttac gacgctggtg gcatcgggct
      601 tgcgcaacaa ggaagtggcg gcccggctcg gcctgtcgga gaaaaccgtc aagatgcacc
      661 gcgggctggt gatggaaaag ctcaacctga agaccagcgc cgatctggtg cgcattgccg
      721 tcgaagccgg aatctaagga tccaaactcg agtaaggatc tccaggcatc aaataaaacg
      781 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      841 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//
