LOCUS       sequence-213361-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector encoding the EL222 gene under constitutive promoter J23106..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Jayaraman P, Yeoh JW, Jayaraman S, Teh AY, Zhang J, Poh CL
  TITLE     Cell-Free Optogenetic Gene Expression System.
  JOURNAL   ACS Synth Biol. 2018 Apr 20;7(4):986-994. doi: 
            10.1021/acssynbio.7b00422. Epub 2018 Apr 4.
  PUBMED    29596741
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00422"; journalName: "ACS Synth Biol"; date: 
            "2018-04-20- 20"; volume: "7"; issue: "4"; pages: "986-994"
COMMENT     Sequence Label: pEBLindv2
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..76
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             83..760
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      793..864
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      880..907
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggtagccttt agtccatgtt agcgaagaaa atggtttgtt atagtcgaat aaatatgtct
       61 agagaaagag gagaaatact agatggcgag tagcgaagac gttatcaaag agttcatgcg
      121 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      181 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      241 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      301 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      361 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      421 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      481 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      541 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      601 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      661 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      721 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa ggatccaaac tcgagtaagg
      781 atctccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      841 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      901 ttctgcgttt ata
//
