LOCUS       sequence-213369-        9160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu 
            S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell
            RE
  TITLE     Optogenetic control with a photocleavable protein, PhoCl.
  JOURNAL   Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222.
  PUBMED    28288123
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 13. doi: 10.1038/nmeth.4222."
COMMENT     Sequence Label: pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
FEATURES             Location/Qualifiers
     source          1..9160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1736..1745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1745..2680
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2696..3421
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
     CDS             4415..5140
                     /codon_start=1
                     /product="photocleavable protein that separates into two 
                     fragments upon exposure to violet light (Zhang et al., 
                     2017)"
                     /label=PhoCl
                     /translation="VIPDYFKQSFPEGYSWERSMTYEDGGICIATNDITMEGDSFINKI
                     HFKGTNFPPNGPVMQKRTVGWEASTEKMYERDGVLKGDVKMKLLLKGGGHYRCDYRTTY
                     KVKQKPVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVARNSTDSMDELYKGGSGGMVS
                     KGEETITSVIKPDMKNKLRMEGNVNGHAFVIEGEGSGKPFEGIQTIDLEVKEGAPLPFA
                     YDILTTAFHYGNRVFTKYPR"
     CDS             5153..6088
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5201..6094
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(6182..6201)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6247..6302
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6301..6320)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6663..6679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6663..6679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6676..6698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6687..6703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6711..6717,6718..6735,6736..6741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6756..6777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6836..7031
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6882..7017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7037..7171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7074..7093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7128..7147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7239..7256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7410..7998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7490..7509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8169..8960,8961..9029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASWQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYMTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8792..8811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9030..9134)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcccgggt gagccgccac
     1741 catggctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
     1801 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
     1861 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
     1921 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
     1981 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
     2041 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
     2101 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
     2161 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
     2221 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
     2281 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
     2341 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
     2401 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
     2461 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
     2521 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
     2581 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
     2641 gtattacatc acgggggagg cagagggttt ccctgccaca gctgtcgacg gtaccgtgat
     2701 ccctgactac ttcaagcaga gcttccccga gggctacagc tgggagcgca gcatgaccta
     2761 cgaggacggc ggcatctgca tcgccaccaa cgacatcaca atggaggggg acagcttcat
     2821 caacaagatc cacttcaagg gcacgaactt cccccccaac ggccccgtga tgcagaagag
     2881 gaccgtgggc tgggaggcca gcaccgagaa gatgtacgag cgcgacggcg tgctgaaggg
     2941 cgacgtgaag atgaagctgc tgctgaaggg cggcggccac tatcgctgcg actaccgcac
     3001 cacctacaag gtcaagcaga agcccgtaaa gctgcccgac taccacttcg tggaccaccg
     3061 catcgagatc ctgagccacg acaaggacta caacaaggtg aagctgtacg agcacgccgt
     3121 ggcccgcaac tccaccgaca gcatggacga gctgtacaag ggtggcagcg gtggcatggt
     3181 gagcaagggc gaggagacca ttacaagcgt gatcaagcct gacatgaaga acaagctgcg
     3241 catggagggc aacgtgaacg gccacgcctt cgtgatcgag ggcgagggca gcggcaagcc
     3301 cttcgagggc atccagacga ttgatttgga ggtgaaggag ggcgccccgc tgcccttcgc
     3361 ctacgacatc ctgaccaccg ccttccacta cggcaaccgc gtgttcacca agtacccacg
     3421 ggctagcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg
     3481 ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc
     3541 atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta
     3601 tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca
     3661 gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc
     3721 actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc
     3781 tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg
     3841 ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat
     3901 agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt
     3961 aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag
     4021 cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc
     4081 gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag
     4141 ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg
     4201 cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg
     4261 agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg
     4321 ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc
     4381 aatggtgcgc ctgctggaag atggcgattc cggagtgatc cctgactact tcaagcagag
     4441 cttccccgag ggctacagct gggagcgcag catgacctac gaggacggcg gcatctgcat
     4501 cgccaccaac gacatcacaa tggaggggga cagcttcatc aacaagatcc acttcaaggg
     4561 cacgaacttc ccccccaacg gccccgtgat gcagaagagg accgtgggct gggaggccag
     4621 caccgagaag atgtacgagc gcgacggcgt gctgaagggc gacgtgaaga tgaagctgct
     4681 gctgaagggc ggcggccact atcgctgcga ctaccgcacc acctacaagg tcaagcagaa
     4741 gcccgtaaag ctgcccgact accacttcgt ggaccaccgc atcgagatcc tgagccacga
     4801 caaggactac aacaaggtga agctgtacga gcacgccgtg gcccgcaact ccaccgacag
     4861 catggacgag ctgtacaagg gtggcagcgg tggcatggtg agcaagggcg aggagaccat
     4921 tacaagcgtg atcaagcctg acatgaagaa caagctgcgc atggagggca acgtgaacgg
     4981 ccacgccttc gtgatcgagg gcgagggcag cggcaagccc ttcgagggca tccagacgat
     5041 tgatttggag gtgaaggagg gcgccccgct gcccttcgcc tacgacatcc tgaccaccgc
     5101 cttccactac ggcaaccgcg tgttcaccaa gtacccacgg gtcgagccat ctgctggaga
     5161 catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag
     5221 cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc
     5281 catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt
     5341 actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc
     5401 aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga
     5461 gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc
     5521 tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt
     5581 cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt
     5641 tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac
     5701 cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac
     5761 tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc
     5821 ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct
     5881 gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga
     5941 cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca
     6001 aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac
     6061 gggggaggca gagggtttcc ctgccacagc ttgatagcgg ccgcactcct caggtgcagg
     6121 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     6181 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     6241 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     6301 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     6361 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     6421 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     6481 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     6541 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     6601 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
     6661 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6721 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6781 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc
     6841 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6901 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6961 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7021 gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7141 actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg
     7201 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7261 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7381 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7741 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7861 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7921 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8161 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8221 tcatccatag ttgcctgact ccccgtcatg tagataacta cgatacggga gggcttacca
     8281 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8341 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8401 tccatccagt ctattaattg ttgccaggaa gctagagtaa gtagttcgcc agttaatagt
     8461 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8521 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8581 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8641 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8701 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8761 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8821 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8881 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8941 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9001 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9061 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9121 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
