LOCUS       sequence-213380-        2658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector based on the TetR/Pip OFF system. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, 
            Sander P, Hilbi H, Seeger MA
  TITLE     A uniform cloning platform for mycobacterial genetics and protein 
            production.
  JOURNAL   Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5.
  PUBMED    29934571
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-22"; volume: "8(1):9539. doi"; pages: " 
            10.1038/s41598-018-27687-5"
COMMENT     Sequence Label: pCOND (Hyg)
FEATURES             Location/Qualifiers
     source          1..2658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(388..405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(559..1147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(639..658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaaaggccct ggcatcctcg atcatctcct ccagggtggg tcggccgggc ttccatccca
       61 gctcggcaag gatcaacgag gacagtcgca cgacgaagtt cttctggatc gcgcccgtgc
      121 tggaagcact caacctcgaa gcgtgtggtt gcggagccat ctagcaacca cacgaggatc
      181 cagatctcca tcctgacgga tggccttacg agttccgaga tcaccccggt cacggtggac
      241 ggcgtcacct tctacgacct gaccgcggag aaatagcgct gtacagcgta tgggaatctc
      301 ttgtacggtg tacgagtatc ttcccgtaca ccgtacaagg agcctgaatg agttgaagag
      361 cggatccgct cttcagcatt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1381 tgcctgactc cccgacgtgg ccgaccagcc cgtcatcgtc aacgcctgat ccgcggtgcg
     1441 gacaggccgt gtcgcgaccg gccgtgcgga attaagccgg cccgtaccct gtgaatagag
     1501 gtccgctgtg acacaagaat ccctgttact tctcgaccgt attgattcgg atgattccta
     1561 cgcgagcctg cggaacgacc aggaattctg ggagccgctg gcccgccgag ccctggagga
     1621 gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc gagagcacca accccgtact
     1681 ggtcggcgag cccggcccgg tgatcaagct gttcggcgag cactggtgcg gtccggagag
     1741 cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac gccccggtgc cggtgccccg
     1801 cctcctcggc cgcggcgagc tgcggcccgg caccggagcc tggccgtggc cctacctggt
     1861 gatgagccgg atgaccggca ccacctggcg gtccgcgatg gacggcacga ccgaccggaa
     1921 cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc ggccggctgc acagggtgcc
     1981 gctgaccggg aacaccgtgc tcacccccca ttccgaggtc ttcccggaac tgctgcggga
     2041 acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc tacctctcgc cccggctgct
     2101 ggaccgcctg gaggactggc tgccggacgt ggacacgctg ctggccggcc gcgaaccccg
     2161 gttcgtccac ggcgacctgc acgggaccaa catcttcgtg gacctggccg cgaccgaggt
     2221 caccgggatc gtcgacttca ccgacgtcta tgcgggagac tcccgctaca gcctggtgca
     2281 actgcatctc aacgccttcc ggggcgaccg cgagatcctg gccgcgctgc tcgacggggc
     2341 gcagtggaag cggaccgagg acttcgcccg cgaactgctc gccttcacct tcctgcacga
     2401 cttcgaggtg ttcgaggaga ccccgctgga tctctccggc ttcaccgatc cggaggaact
     2461 ggcgcagttc ctctgggggc cgccggacac cgcccccggc gcctgacgcc ccgggccgcc
     2521 cggcgccgcc cccggccccc ggcggccggc cgccgggcgg agccccgggc ccgcgccgaa
     2581 gcccgctgct gcgagcccgg agcgggccgg ccgacggcgg tgcgggcccg gcggcggacg
     2641 ctcagcagcg gcgggcgt
//
