LOCUS       sequence-213382-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of genetically encoded green fluorescent 
            potassium ion indicator GINKO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Shen Y, Wu SY, Rancic V, Aggarwal A, Qian Y, Miyashita SI, Ballanyi 
            K, Campbell RE, Dong M
  TITLE     Genetically encoded fluorescent indicators for imaging intracellular
            potassium ion concentration.
  JOURNAL   Commun Biol. 2019 Jan 14;2:18. doi: 10.1038/s42003-018-0269-2. 
            eCollection 2019.
  PUBMED    30652129
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-01-14"; volume: "2:18. doi"; pages: " 
            10.1038/s42003-018-0269-2. eCollection 2019"
COMMENT     Sequence Label: pBAD-GINKO1
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1101..1122
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacctgcc ggacggcctg ttcaacttcg tgaaggacgc cggcgagaag
      481 ctgtgggatg ccgtgacagg ccagcacgac aaggacgacc aggccaagaa ggtgcaggaa
      541 cacctgaaca agaccggcat ccccgacgcc gacaaagtga acatccagat cgccgacggc
      601 aaggccacag tgacaggcga cggactgagc caggaagcca aagaaaagat cctggtggcc
      661 gtgggcaaca tcagcggaat cgccagcgtg gacgatcaag tgaaaaccgc cacccctgcc
      721 accgccagcc agttctacac agtgaagtcc ggcgacaccc tgagcgccat cagcaaacag
      781 gtgtacggca acgccaacct gtacaacaag attttcgagg ccaacaagcc catgctgaag
      841 tcccccgaca aaatctaccc cggccaggtg ctgagaatcc ccgaggagct ggagaacgtc
      901 tatatcaagg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac
      961 atcgaggacg gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac
     1021 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac
     1081 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1141 ctcggcatgg acgagctgta caagtaa
//
