LOCUS       sequence-213406-        7101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Nacu E, Gromberg E, Oliveira CR, Drechsel D, Tanaka EM
  TITLE     FGF8 and SHH substitute for anterior-posterior tissue interactions 
            to induce limb regeneration.
  JOURNAL   Nature. 2016 May 19;533(7603):407-10. doi: 10.1038/nature17972.
  PUBMED    27120163
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-05-19- 19"; volume: "533"; issue: "7603"; 
            pages: "407-10"
COMMENT     Sequence Label: pOEM1_pCMV:axFgf8-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     495..514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     687..704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1056..1428
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1529..1832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1833..2036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1987..2007
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2794..2814)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3212..3223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3458..3592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3495..3514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3549..3568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3705..3737)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(3933..3992)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4055..4146)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4113..4130)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4232..4837
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4875..4999)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5609..5628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5610..5628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5639..5656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5639..5655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5648..5670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        5971..6075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6076..6144,6145..6936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6294..6313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
       61 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca ttaggcaccc
      661 caggctttac ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gagagcgccc
      721 aatacgcaag gaaacagcta tgaccatgtt aatgcagctg gcacgacagg tttcccgact
      781 ggaaagcggg cagtgagcgg aaggcccatg aggcccaaaa agctcaacta caaatttgat
      841 cgcggacgtg ttagccgaca caattaatag gcgtcgtgtg gctatggcaa aatcgtcttc
      901 ggaagcaact tctaacgacg agggttggga cgacgacgat aatcggccta ataaagctaa
      961 cacgcccgat gttaaatatg tccaagcgtt atttaacgtg tttacgtcga gtcaattgta
     1021 cactaacgac agtgatgaaa gaaatacaaa agcgcataat attttgaacg acgtcgaacc
     1081 tttattacaa aacaaaacac aaacgaatat cgacaaagct agattgctgc tacaagattt
     1141 ggcaagtttt gtggcgttga gcgaaaatcc attagatagt ccagccatcg gttcggaaaa
     1201 acaacccttg tttgaaacta atcgaaacct attttacaaa tctattgagg atttaatatt
     1261 taaattcaga tataaagacg ctgaaaatca tttgattttc gctctaacat accaccctaa
     1321 agattataaa tttaatgaat tattaaaata cgtacaacaa ttgtctgtaa atcaacaacg
     1381 cacagaatct agcgcttaat aaatgtacta ataacaatgt atcgtgtttt attaattaag
     1441 atctgcggat gcaggctccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     1501 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     1561 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     1621 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1681 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1741 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1801 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     1861 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1921 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1981 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     2041 tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atgaacaacg cgagctcgtc
     2101 agtcgtcgga tatctattgc tgcatctttt tgtcctctgc atgcaagcaa aggtaactgt
     2161 tcagtcccca cctaatttta cacagcatgt gagggagcag agcctggtga cagaccagct
     2221 gagccggcgg ctcatccgca cctaccagct gtacagccgc accagtggga agcacgtgca
     2281 ggtccttggc aacaagaaga tcaacgccat ggccgacgac ggcgactcgc acgccaaatt
     2341 aattgtggaa acggacacct tcggaagccg cgtccgcatt aaaggagccg agacgggcta
     2401 ttacatctgc atgaacaaga agggaaagct gattggcaag agcaatggcc gaggcaaaga
     2461 ctgcgtcttc tccgagatag tgctcgagaa caactacacg gcactacaaa acgccaaata
     2521 cgaaggatgg tacatggcct tcacaaggaa aggccgcccg aggaaaggct ccaagaccag
     2581 gcagcaccag cgggaggtcc acttcatgaa gcgactgccg aaaggccacc aaacaacgga
     2641 gcctcataga cgcttcgagt ttgtaaatta tcctttcaac aggcggagca aaagaactcg
     2701 acattccgta ccgcgatagg cggccgctag acgcgtcgac aatcaacctc tggattacaa
     2761 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2821 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2881 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2941 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3001 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3061 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3121 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     3181 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3241 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3301 tcggatctcc ctttgggccg cctccccgaa gcttgtcgag aagtactaga ggatcataat
     3361 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3421 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     3601 ggcgcgccgt gatgcgtatt tctgaatctt tgtaaaatag cacacaagac tccaacgcgt
     3661 ttggcgtttt attttcttgc tcgactctag tttattaggc cttactttcc aagtcggttc
     3721 atctctatgt ctgtataaat ctgtcttttc ttggtgtgct ttaatttaat gcaaagatgg
     3781 ataccaactc ggagaaccaa gaatagtcca atgattaacc ctatgataaa gaaaaaagag
     3841 gcaatagagc ttttccaact actgaaccaa ccttctacaa gctcgattgg atttttggat
     3901 agcccagtat caccccgggg tacctgcaga tccgcaaagg cagaatgcgc cgccgccgcc
     3961 aaaagcacat ataaaacaat agcgcttacc atcttgcttg tgtgttcctt attgaagcct
     4021 tggtgtgact gatttactag tagcatagat ccgtatttat aggttttttt attacaaaac
     4081 tgttacgaaa acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc
     4141 catgatccag ccaaccacta gaactatagc tagagtcctg ggcgaacaaa atggtggcag
     4201 cttgtgatat tagtttgtgc gtctcattac aatggctgtt atttttaaca acaaacaact
     4261 gctcgcagac aatagtatag aaaagggagg tgaactgttt ttgtttaacg gttcgtacaa
     4321 cattttggaa agttatgtta atccggtgct gctaaaaaat ggtgtaattg aactagaaga
     4381 agctgcgtac tatgccggca acatattgta caaaaccgac gatcccaaat tcattgatta
     4441 tataaattta ataattaaag caacacactc cgaagaacta ccagaaaata gcactgttgt
     4501 aaattacaga aaaactatgc gcagcggtac tatacacccc attaaaaaag acatatatat
     4561 ttatgacaac aaaaaattta ctctatacga tagatacata tatggatacg ataataacta
     4621 tgttaatttt tatgaggaga aaaatgaaaa agagaaggaa tacgaagaag aagacgacaa
     4681 ggcgtctagt ttatgtgaaa ataaaattat attgtcgcaa attaactgtg aatcatttga
     4741 aaatgatttt aaatattacc tcagcgatta taactacgcg ttttcaatta tagataatac
     4801 tacaaatgtt cttgttgcgt ttggtttgta tcgttaataa aaaacaaatt tagcatttat
     4861 aattgtttta ttattcaata attacaaata ggattgagac ccttgcagtt gccagcaaac
     4921 ggacagagct tgtccacagt tttcattttt tggcagtttg gtggtttata cacatcttgg
     4981 gatttgatta aaagatgcat gacttcaccc acacacttgg agtcgtggtt gtagaaatgc
     5041 gtcagagcct tgattaaaca tcggttttca cacttggcac acgaaaccac gtttgcaatg
     5101 taacagttta gtataaacct cttcctgtac ataccgccgc gcggtacatt gttttctttc
     5161 aaatcgttca atattttttt tatacacgga ggcatattaa tcttgttgtt cagcatgtcc
     5221 acgacatttt tctttccttc tacgcattct ttcatacaaa tgtttctctt gctgcgtttt
     5281 ttaaaattgg aatttttaat ttcattaata gtgggcggag ccgttaacag catgtacaat
     5341 cgcagtccgg aaattttgac caaaaccccg ccattgtaga ataccgtgta tggagttagg
     5401 gtcaatttat caataaaatc atcgggatca attaaatatg tagctttctt gtctaaacat
     5461 gacgctctaa tgagcggact ccacacgtta tacgatgcat tcgccatact tcgcggcttc
     5521 tcgcaccagc ggtcctgatt cgactaacgg tcgattggat ggcttaatag ttttaacgtc
     5581 acggccttga cggccttccg ccaattcgcc ctatagtgag tcgtattacg tcgcgctcac
     5641 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5701 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgaaacgc
     5761 ccttcccaac agttgcgcag cctgaatggc gaatgggagc gccctgtagc ggccactcaa
     5821 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5881 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     5941 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6061 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6121 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6181 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6241 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6301 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6361 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6421 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6481 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6541 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6601 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6661 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6721 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6781 ggtgagcgtg gttctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6841 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6901 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6961 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7021 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7081 cccgtagaaa agatcaaagg a
//
