LOCUS       sequence-21344-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Kumar MS, Lu J, Mercer KL, Golub TR, Jacks T
  TITLE     Impaired microRNA processing enhances cellular transformation and 
            tumorigenesis.
  JOURNAL   Nat Genet. 2007 May;39(5):673-7. doi: 10.1038/ng2003.
  PUBMED    17401365
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Genet"; date: "2007-05"; volume: "39"; issue: "5"; pages: 
            "673-7"
COMMENT     Sequence Label: pSicoR mouse Dicer3
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        114..613
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(149..172)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     546..565
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(657..676)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cttgtttgca tggtggtgtc gatatttctc ttgaaaatat cgacaccacc atgctttttt
       61 ctcgagtact aggatcatta ggcggccgaa ttcgtcgacc tcgaaattct accgggtagg
      121 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
      181 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
      241 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
      301 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
      361 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
      421 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
      481 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
      541 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
      601 tccgggcctt tcgacctgca tccatctaga tctcgatcga gcagctgaag cttaccatga
      661 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
      721 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
      781 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ct
//
