LOCUS       sequence-213452-       11177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL2-deltaTM
FEATURES             Location/Qualifiers
     source          1..11177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1259..1261,1262..1264,1265..1975)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1304..1325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1565..1584)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1912..1933
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3436..3460)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3491..4079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3544..3564)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3962..3973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4130..4629
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4165..4188)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4562..4581
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4643..5446
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4706..4725)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5316..5335
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5590..5823
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5895..6016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5932..5951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5986..6005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6056..6191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6212..6230)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6240..6257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6240..6256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6249..6271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6398..6853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6485..6504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6695..6716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6879..6983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6984..7052,7053..7844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7202..7221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8015..8603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8504..8523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8757..8774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8891..8912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8927..8932,8933..8950,8951..8957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8965..8981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8970..8992
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8989..9005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8989..9005
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9024..9044
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9026..9044
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9072..9298
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9299..9479
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9526..9651
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10144..10377
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10562..10606
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10873..10989
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    11023..11047
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccat
     1261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1321 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1441 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1561 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1621 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1681 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1741 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1801 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1861 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1921 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg
     1981 atctggaggt tctggtggaa tggcggaggg ggacgaggca gcgcgagggc agcaaccgca
     2041 ccaggggctg cggcgccggc gacggaccag cgacccaagc gccgcggtta accacgtctc
     2101 gtccacgacc tccctaggtg agaattatga agatgatgac ctagtaaatt ctgatgaggt
     2161 tatgaagaaa ccatgtccag tacagattgt tcttgctcat gaagatgacc ataactttga
     2221 acttgatgaa gaagctttgg agcagatatt gctacaggag cacatacgag atcttaacat
     2281 agtagtggta tctgtggcag gagcttttcg taaagggaag tcatttctac tggacttcat
     2341 gcttagatac atgtataaca aggattctca aagttggatt ggtggaaaca atgaaccatt
     2401 gacaggcttt acatggcgag gtggctgtga aagagaaaca acaggcatac aagtttggaa
     2461 tgaagtattt gtgattgaca gacctaatgg aactaaagtt gctgtgctgc ttatggatac
     2521 ccagggtgcc tttgatagcc agtcaactat caaagactgt gcaacggtgt ttgctctgag
     2581 cactatgact agctctgtcc aggtatataa tctgtctcag aatattcaag aagatgatct
     2641 tcaacatttg caattattta cagagtatgg aagacttgcg atggaagaaa tctaccagaa
     2701 accatttcag acattaatgt ttttgattcg agattggagc tatccttatg aacattcata
     2761 tggtttggaa ggtggaaagc aatttcttga aaagagatta caggtaaaac aaaatcaaca
     2821 tgaagagctt cagaatgtaa ggaagcacat acacaattgt ttctcaaatc ttggttgctt
     2881 ccttttgcca catcctggtc ttaaagttgc aactaatcct agttttgatg ggagattgaa
     2941 agatattgat gaagacttta aacgcgagct tcgaaatctg gttccattgc tgcttgcccc
     3001 tgaaaatttg gtagaaaaag agataagtgg atctaaagtc acttgtagag atcttgtaga
     3061 atattttaag gcttacatca aaatctatca aggagaagaa cttccacatc caaagtccat
     3121 gcttcaggca acagctgaag ctaataatct tgctgcagta gcaggagcaa gagataccta
     3181 ttgtaaaagt atggaacagg tatgtggagg ggacaagcct tacattgcac cttcagatct
     3241 ggagcgaaaa cacttggatc tcaaggaagt ggcgataaaa caatttcgtt cagtaaaaaa
     3301 gatgggtgga gatgagttct gccgtcgtta tcaggaccag cttgaagctg aaattgaaga
     3361 aacctatgca aattttataa agcacaatga tggcaaaaat atcttctatg ctgctcgtac
     3421 cccagcctga tctagaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg
     3481 gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3541 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3601 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3661 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3721 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3781 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3841 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     3901 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3961 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4021 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4081 tggacctgca ggattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag
     4141 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     4201 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     4261 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     4321 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     4381 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     4441 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     4501 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     4561 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     4621 ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg
     4681 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     4741 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     4801 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     4861 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4921 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4981 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5041 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5101 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5161 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5221 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5281 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5341 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5401 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaacgc gtcgcccgcc
     5461 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg
     5521 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5581 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     5641 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5701 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5761 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5821 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5881 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6001 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6061 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6121 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6181 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6361 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6841 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7081 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7141 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7201 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7261 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7321 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7381 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7441 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7501 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7561 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7621 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7681 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7741 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7801 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8761 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8821 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8881 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8941 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9001 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9061 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     9121 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     9181 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     9241 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     9301 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9361 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9421 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9481 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9541 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9601 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9661 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9721 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9781 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9841 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9901 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9961 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
    10021 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    10081 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    10141 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    10201 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10261 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10321 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10381 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10441 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10501 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10561 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10621 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10681 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10741 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    10801 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    10861 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    10921 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt
    10981 caaaatttta tcgtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
    11041 gcaggctcca ccatgggaac cgacattgat tattgactag gcttttgcaa aaagctttgc
    11101 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
    11161 tgaaaggagt gggaatt
//
