LOCUS       sequence-213458-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Id1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Cunningham TJ, Yu MS, McKeithan WL, Spiering S, Carrette F, Huang 
            CT, Bushway PJ, Tierney M, Albini S, Giacca M, Mano M, Puri PL, 
            Sacco A, Ruiz-Lozano P, Riou JF, Umbhauer M, Duester G, Mercola M, 
            Colas AR
  TITLE     Id genes are essential for early heart formation.
  JOURNAL   Genes Dev. 2017 Aug 9. pii: gad.300400.117. doi: 
            10.1101/gad.300400.117.
  PUBMED    28794185
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2017 Aug 9. pii: gad.300400.117. doi: 10.1101/gad.300400.117."
COMMENT     Sequence Label: pCDH-EFIa-Id1-PGK-puro
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..24
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16..33
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17..33
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        48..274
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             275..455
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    502..627
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1120..1353
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1538..1582
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1849..1965
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2014..2225
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2238..2506
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        2577..2788
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2801..3069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        3140..3351
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3364..3632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        4154..4664
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             4686..5285
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4686..4705)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5182..5202
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5292..5880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5345..5365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5763..5774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5954..6187
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6259..6380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6296..6315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6350..6369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6420..6555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6588..6604)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6588..6604)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6601..6623)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6612..6628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6636..6642,6643..6660,6661..6666))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6681..6702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6819..6836)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6990..7578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7749..8540,8541..8609))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8372..8391
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8610..8714)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8782..8800
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8838..8860)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8960..8979
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt agtcttatgc
       61 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      121 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      181 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      241 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      301 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      361 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      421 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      481 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      541 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      601 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      661 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      721 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      781 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
      841 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
      901 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
      961 caagcggcca ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1021 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1081 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1141 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1201 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1261 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1321 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1381 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1441 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1501 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1561 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1621 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1681 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1741 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1801 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     1861 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1921 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga tactagtccg
     1981 caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     2041 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
     2101 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     2161 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     2221 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
     2281 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
     2341 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
     2401 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
     2461 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg
     2521 cgcctacgct agactctagt ccgcaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     2581 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     2641 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2701 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2761 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     2821 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     2881 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     2941 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     3001 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     3061 ttacagatcc aagctgtgac cggcgcctac gctagactct agtccgcaag gatctgcgat
     3121 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     3181 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3241 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3301 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     3361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     3421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     3481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     3541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     3601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     3661 tctagagcca ccatgaaggt cgccagtggc agtgccgcag ccgctgcagg ccctagctgt
     3721 tcgctgaagg cgggcaggac agcgggcgag gtggtacttg gtctgtcgga gcaaagcgtg
     3781 gccatctcgc gctgcgctgg gacgcgcctg cccgccttgc tggacgagca gcaggtgaac
     3841 gtcctgctct acgacatgaa cggctgctac tcacgcctca aggagctggt gcccaccctg
     3901 ccccagaacc gcaaagtgag caaggtggag atcctgcagc atgtaatcga ctacatcagg
     3961 gacctgcagc tggagctgaa ctcggagtct gaagtcggga ccaccggagg ccggggactg
     4021 cctgtccgcg ccccgctcag caccctgaac ggcgagatca gtgccttggc ggccgaggcg
     4081 gcatgtgttc cagccgacga tcgcatcttg tgtcgctgag aattcgaatt taaatcggat
     4141 ccgcggccgc cacggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     4201 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     4261 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     4321 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     4381 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     4441 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     4501 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     4561 gggccctgtt cctgcccgcg cggtgttccg cactctgcaa gcctccggag cgcacgtcgg
     4621 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccaggggaat ccaccggacg
     4681 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     4741 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgttgacc
     4801 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4861 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4921 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4981 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     5041 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     5101 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     5161 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     5221 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     5281 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5341 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5401 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5461 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5521 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5581 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5641 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5701 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5761 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5821 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5881 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5941 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct
     6001 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6061 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6121 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6181 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     6241 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6361 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6481 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6541 gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc atagctgttt
     6601 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6661 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6721 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6781 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6841 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7861 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8041 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8221 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8761 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     8821 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     8881 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     8941 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     9001 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     9061 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     9121 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt t
//
