LOCUS       sequence-213494-       11096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL1-G20TM
FEATURES             Location/Qualifiers
     source          1..11096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(80..82,83..85,86..796)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(80..82,83..85,86..796)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(125..146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(386..405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     733..754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2176..2200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2231..2819
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2284..2304)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2702..2713)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2870..3369
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2905..2928)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3302..3321
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3383..4186
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3446..3465)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4056..4075
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4330..4563
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4635..4756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4672..4691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4726..4745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4796..4931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4952..4970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4980..4997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4980..4996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4989..5011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5138..5593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5225..5244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5435..5456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5619..5723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5724..5792,5793..6584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5942..5961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6755..7343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7244..7263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7497..7514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7631..7652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7667..7672,7673..7690,7691..7697)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7705..7721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7710..7732
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7729..7745
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7729..7745
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7764..7784
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7766..7784
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7812..8038
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8039..8219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8266..8391
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8884..9117
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9302..9346
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9613..9729
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9763..9787
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9918..11096
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10149..11087
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11044..11064
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaattagct tggtactaat acgactcact atagggagac ccaagctggc taggtaagct
       61 tggtaccgag ctcggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      541 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      781 ggacgagctg tacaagggtg gatctggagg ttctggcgga atggccaaga accgcaggga
      841 cagaaacagt tggggtggat tttcggaaaa gacatatgaa tggagctcag aagaggagga
      901 gccagtgaaa aaggcaggac cagtccaagt cctcattgtc aaagatgacc attcctttga
      961 gttagatgaa actgcattaa atcggatcct tctctcggag gctgtcagag acaaggaggt
     1021 tgttgctgta tctgttgctg gagcatttag aaaaggaaaa tcattcctga tggacttcat
     1081 gttgagatac atgtacaacc aggaatcagt tgattgggtt ggagactaca atgaaccatt
     1141 gactggtttt tcatggagag gtggatctga acgagagacc acaggaattc agatatggag
     1201 tgaaatcttc cttatcaata aacctgatgg taaaaaggtt gcagtgttat tgatggatac
     1261 tcagggaacc tttgatagtc agtcaacttt gagagattca gccacagtat ttgcccttag
     1321 cacaatgatc agctcaatac aggtatataa cttatcccaa aatgtccagg aggatgatct
     1381 tcagcacctc cagcttttca ctgagtatgg cagactggca atggaggaaa cattcctgaa
     1441 gccatttcag agtctgatat ttcttgttcg agactggagt ttcccatacg aattttcata
     1501 tggagccgat ggtggtgcca aattcttgga aaaacgcctc aaggtctcag ggaaccagca
     1561 tgaagaacta cagaacgtca gaaaacacat ccattcctgt ttcaccaaca tttcctgttt
     1621 tctgctacct catcctggct taaaagtagc taccaatcca aactttgatg gaaaattgaa
     1681 agaaatagat gatgaattca tcaaaaactt gaaaatactg attccttggc tacttagtcc
     1741 cgagagccta gatattaaag agatcaatgg gaataaaatc acctgccggg gtctggtgga
     1801 gtacttcaag gcttatataa agatctatca aggtgaagaa ttaccacatc ccaaatccat
     1861 gttacaggcc acagcagaag ctaacaattt agcagccgtg gcaactgcca aggacacata
     1921 caacaaaaaa atggaagaga tttgtggtgg tgacaaacca tttctggccc caaatgactt
     1981 gcagaccaaa cacctgcaac ttaaggaaga atctgtgaag ctattccgag gggtgaagaa
     2041 gatgggtggg gaagaattta gccggcgtta cctgcagcag ttggagagtg aaatagatga
     2101 actttacatc caatatatca agcacaatga tagcaaaaat atcttccatg cagctcgtac
     2161 cccagcctaa tctagaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg
     2221 gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2281 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2341 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2401 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2461 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2521 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2581 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     2641 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2701 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2761 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     2821 tggacctgca ggattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag
     2881 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2941 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     3001 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     3061 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     3121 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     3181 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     3241 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     3301 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     3361 ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg
     3421 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3481 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     3541 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     3601 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     3661 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     3721 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     3781 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     3841 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     3901 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     3961 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4021 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4081 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4141 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaacgc gtcgcccgcc
     4201 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg
     4261 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4321 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     4381 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4441 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4501 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4561 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     4621 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4681 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4741 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4801 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4861 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4921 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4981 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5041 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5101 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     5161 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5221 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5281 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5341 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5401 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5461 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5521 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5581 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5641 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5701 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5761 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5821 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5881 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5941 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6001 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6061 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6121 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6181 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6241 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6301 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6361 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6421 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6481 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6541 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6601 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6661 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6721 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6781 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6841 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6901 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6961 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7021 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7081 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7141 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7201 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7261 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7321 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7381 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7441 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7501 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7561 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7621 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7681 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7741 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7801 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7861 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     7921 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7981 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     8041 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8101 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8161 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8221 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8281 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8341 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     8401 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     8461 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     8521 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     8581 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8641 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8701 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8761 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8821 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8881 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8941 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9001 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9061 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     9121 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     9181 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     9241 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     9301 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     9361 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     9421 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     9481 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     9541 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     9601 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     9661 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt
     9721 caaaatttta tcgtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     9781 gcaggctcca ccatgggaac cgacattgat tattgactag gcttttgcaa aaagctttgc
     9841 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     9901 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     9961 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
    10021 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
    10081 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
    10141 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
    10201 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
    10261 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
    10321 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
    10381 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
    10441 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
    10501 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
    10561 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
    10621 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
    10681 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
    10741 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
    10801 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
    10861 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
    10921 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
    10981 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
    11041 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtga
//
