LOCUS       sequence-213502-       11240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-FAM134B
FEATURES             Location/Qualifiers
     source          1..11240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(80..82,83..85,86..796)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(80..82,83..85,86..796)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(125..146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(386..405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     733..754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2320..2344)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2375..2963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2428..2448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2846..2857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3014..3513
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3049..3072)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3446..3465
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3527..4330
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3590..3609)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4200..4219
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4474..4707
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4779..4900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4816..4835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4870..4889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4940..5075
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5096..5114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5124..5141)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5124..5140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5133..5155)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5282..5737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5369..5388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5579..5600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5763..5867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5868..5936,5937..6728)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6086..6105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6899..7487
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7388..7407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7641..7658
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7775..7796
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7811..7816,7817..7834,7835..7841)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7849..7865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7854..7876
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7873..7889
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7873..7889
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7908..7928
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7910..7928
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7956..8182
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8183..8363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8410..8535
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9028..9261
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9446..9490
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9757..9873
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9907..9931
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        10062..11240
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10293..11231
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11188..11208
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaattagct tggtactaat acgactcact atagggagac ccaagctggc taggtaagct
       61 tggtaccgag ctcggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      541 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      781 ggacgagctg tacaagggtg gatctggagg ttctggtgga atggcgagcc cggcgcctcc
      841 ggagcacgcc gaggagggat gcccggctcc tgccgccgag gagcaggcgc cgccgtcgcc
      901 gccaccgccc caggcatccc ccgcagagcg gcagcagcag gaggaggaag cgcaggaagc
      961 tggggcggcg gagggcgcgg ggttgcaggt ggaggaggcc gcgggccggg cggcggccgc
     1021 ggtaacctgg ctgctcgggg agccggtgct gtggctgggc tgccgcgccg acgagctgct
     1081 gagctggaag aggccgctgc ggagcctgct cggcttcgtc gctgccaacc tgctgttctg
     1141 gttccttgca ttgactccat ggagagtata tcacctgatt tccgtcatga tacttgggcg
     1201 tgttattatg caaataataa aggatatggt tttgtctaga acaagaggtg cacagttgtg
     1261 gagaagcctc agtgaaagct gggaagttat caattccaaa ccagatgaaa gacccaggct
     1321 cagccactgt attgcagaat catggatgaa tttcagcata tttcttcaag aaatgtctct
     1381 ttttaaacag cagagccctg gcaagttttg tctcctggtc tgtagtgtgt gcacattttt
     1441 tacgatcttg ggaagttaca ttcctggggt tatactcagc tatctactgt tactgtgtgc
     1501 atttttgtgt ccattgttta aatgtaatga tattggacaa aaaatttaca gcaaaattaa
     1561 gtcagttctg ctgaaactgg attttggaat tggagaatat attaatcaga agaaacgtga
     1621 gagatctgaa gcagataaag aaaaaagtca caaagatgac agtgaattag acttttcagc
     1681 tctttgtcct aagattagcc tcacggttgc tgccaaagag ttatctgtgt ctgacacaga
     1741 cgtctcagag gtatcctgga ctgataatgg gaccttcaac ctttcagaag gatacactcc
     1801 acagacagac acttctgatg atcttgaccg acccagtgag gaagttttct ctagagatct
     1861 ttcagatttt ccatctctag aaaatggcat gggaacaaat gatgaagatg aattaagcct
     1921 tggtttgccc actgagctca agagaaagaa ggaacagttg gacagtggtc acagaccaag
     1981 caaagagacg caatcagcag ctggtctcac ccttcctctg aacagtgacc aaacctttca
     2041 cctgatgagc aacctggctg gggatgttat cacagctgca gtgactgcag ctatcaaaga
     2101 ccagttagag ggtgtgcagc aagcactttc tcaggctgcc cccatcccag aagaggacac
     2161 agacactgaa gaaggtgatg actttgaact acttgaccag tcagagctgg atcaaattga
     2221 gagtgaattg ggacttacac aagaccagga agcagaagca cagcaaaata agaagtcttc
     2281 aggtttcctt tcaaatctgc tgggaggcca ttaatctaga cccagctttc ttgtacaaag
     2341 tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg
     2401 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2461 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2521 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2581 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2641 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2701 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2761 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     2821 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2881 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2941 ctccctttgg gccgcctccc cgcctggacc tgcaggattc tgcagatatc cggactagtg
     3001 atctaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     3061 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     3121 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     3181 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     3241 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     3301 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     3361 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     3421 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     3481 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaatatgg gatcggccat
     3541 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3601 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3661 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     3721 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3781 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3841 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3901 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3961 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4021 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4081 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4141 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4201 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4261 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4321 gttcttctga acgcgtcgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     4381 accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
     4441 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
     4501 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4561 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4621 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4681 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     4741 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4921 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4981 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5041 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     5101 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5161 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5221 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5281 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5341 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5401 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5461 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5521 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5581 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5641 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5701 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5761 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5821 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5881 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5941 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6001 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6061 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6121 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6181 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6241 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6301 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6361 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6421 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6481 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6541 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6601 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6661 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6721 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6781 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6841 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6901 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6961 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7021 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7081 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7141 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7261 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7321 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7381 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7441 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7501 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7561 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7621 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7681 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7741 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7801 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7861 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7921 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7981 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     8041 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     8101 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     8161 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     8221 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8281 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8341 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     8401 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     8461 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     8521 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8581 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8641 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8701 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8761 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8821 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8881 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8941 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     9001 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     9061 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     9121 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     9181 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     9241 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     9301 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     9361 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     9421 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9481 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9541 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9601 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9661 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     9721 tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg
     9781 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9841 attacaaaaa caaattacaa aattcaaaat tttatcgtcc agtgtggtgg aattctgcag
     9901 atatcaacaa gtttgtacaa aaaagcaggc tccaccatgg gaaccgacat tgattattga
     9961 ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
    10021 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
    10081 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
    10141 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
    10201 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
    10261 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
    10321 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
    10381 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
    10441 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
    10501 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
    10561 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
    10621 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
    10681 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
    10741 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
    10801 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
    10861 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
    10921 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
    10981 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
    11041 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
    11101 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
    11161 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
    11221 ttccatttca ggtgtcgtga
//
