LOCUS       sequence-213505-        9946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gene expression vector for phospho-MARK3 expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pLX304-MARK3
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     387..404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    521..542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(557..562,563..580,581..587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     600..622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     619..635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     619..635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     654..674
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        656..674
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        702..928
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             929..1109
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1156..1281
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1774..2007
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2192..2236
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2411..2911
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2830..2848
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2923..3318
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3378..3495
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3612..3915
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3916..4119
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4070..4090
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4142..4166
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6391..6415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6417..6458
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6500..7088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6553..6573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6971..6982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7160..7393
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7471..7592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7508..7527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7562..7581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7632..7767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7787..7806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7788..7806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7816..7833)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7816..7832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7825..7847)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7974..8429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8061..8080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8271..8292
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8455..8559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8560..8628,8629..9420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8778..8797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9591..9946,1..233)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
       61 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      601 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      661 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
      721 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
      781 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
      841 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
      901 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
      961 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1021 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1081 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1141 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1201 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1261 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1321 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1381 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1441 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1501 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1561 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1621 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1681 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1741 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1801 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1861 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1921 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1981 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2041 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2101 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2161 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2221 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2281 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2341 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2401 gaggggaccc ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     2461 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     2521 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     2581 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     2641 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     2701 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     2761 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     2821 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     2881 cgttgaccga atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct
     2941 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     3001 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     3061 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     3121 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     3181 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     3241 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     3301 ggttatgtgt gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg
     3361 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     3421 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     3481 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     3541 gagggggccc ggggttatta atagtaatca attacggggt cattagttca tagcccatat
     3601 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     3661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     3721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     3781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     3841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     3901 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     3961 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4021 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4081 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tgtcgggatc
     4141 aacaagtttg tacaaaaaag caggctccac catggagtgc agtaaaatgt ccactaggac
     4201 cccattgcca acggtgaatg aacgagacac tgaaaaccac acgtcacatg gagatgggcg
     4261 tcaagaagtt acctctcgta ccagccgctc aggagctcgg tgtagaaact ctatagcctc
     4321 ctgtgcagat gaacaacctc acatcggaaa ctacagactg ttgaaaacaa tcggcaaggg
     4381 gaattttgca aaagtaaaat tggcaagaca tatccttaca ggcagagagg ttgcaataaa
     4441 aataattgac aaaactcagt tgaatccaac aagtctacaa aagctcttca gagaagtaag
     4501 aataatgaag attttaaatc atcccaatat agtgaagtta ttcgaagtca ttgaaactga
     4561 aaaaacactc tacctaatca tggaatatgc aagtggaggt gaagtatttg actatttggt
     4621 tgcacatggc aggatgaagg aaaaagaagc aagatctaaa tttagacaga ttgtgtctgc
     4681 agttcaatac tgccatcaga aacggatcgt acatcgagac ctcaaggctg aaaatctatt
     4741 gttagatgcc gatatgaaca ttaaaatagc agatttcggt tttagcaatg aatttactgt
     4801 tggcggtaaa ctcgacacgt tttgtggcag tcctccatac gcagcacctg agctcttcca
     4861 gggcaagaaa tatgacgggc cagaagtgga tgtgtggagt ctgggggtca ttttatacac
     4921 actagtcagt ggctcacttc cctttgatgg gcaaaaccta aaggaactga gagagagagt
     4981 attaagaggg aaatacagaa ttcccttcta catgtctaca gactgtgaaa accttctcaa
     5041 acgtttcctg gtgctaaatc caattaaacg cggcactcta gagcaaatca tgaaggacag
     5101 gtggatcaat gcagggcatg aagaagatga actcaaacca tttgttgaac cagagctaga
     5161 catctcagac caaaaaagaa tagatattat ggtgggaatg ggatattcac aagaagaaat
     5221 tcaagaatct cttagtaaga tgaaatacga tgaaatcaca gctacatatt tgttattggg
     5281 gagaaaatct tcagagctgg atgctagtga ttccagttct agcagcaatc tttcacttgc
     5341 taaggttagg ccgagcagtg atctcaacaa cagtactggc cagtctcctc accacaaagt
     5401 gcagagaagt gtttcttcaa gccaaaagca aagacgctac agtgaccatg ctggaccagc
     5461 tattccttct gttgtggcgt atccgaaaag gagtcagacc agcactgcag atagtgacct
     5521 caaagaagat ggaatttcct cccggaaatc aagtggcagt gctgttggag gaaagggaat
     5581 tgctccagcc agtcccatgc ttgggaatgc aagtaatcct aataaggcgg atattcctga
     5641 acgcaagaaa agctccactg tccctagtag taacacagca tctggtggaa tgacacgacg
     5701 aaatacttat gtttgcagtg agagaactac agctgataga cactcagtga ttcagaatgg
     5761 caaagaaaac agcactattc ctgatcagag aactccagtt gcttcaacac acagtatcag
     5821 tagtgcagcc accccagatc gaatccgctt cccaagaggc actgccagtc gtagcacttt
     5881 ccacggccag ccccgggaac ggcgaaccgc aacatataat ggccctcctg cctctcccag
     5941 cctgtcccat gaagccacac cattgtccca gactcgaagc cgaggctcca ctaatctctt
     6001 tagtaaatta acttcaaaac tcacaaggag tcgcaatgta tctgctgagc aaaaagatga
     6061 aaacaaagaa gcaaagcctc gatccctacg cttcacctgg agcatgaaaa ccactagttc
     6121 aatggatccc ggggacatga tgcgggaaat ccgcaaagtg ttggacgcca ataactgcga
     6181 ctatgagcag agggagcgct tcttgctctt ctgcgtccac ggagatgggc acgcggagaa
     6241 cctcgtgcag tgggaaatgg aagtgtgcaa gctgccaaga ctgtctctga acggggtccg
     6301 gtttaagcgg atatcgggga catccatagc cttcaaaaat attgcttcca aaattgccaa
     6361 tgagctaaag ctgtaaccca gtgattatga acccagcttt cttgtacaaa gtggttggta
     6421 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
     6481 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6541 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6601 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6661 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6721 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6781 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6841 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6901 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6961 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7021 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7081 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7141 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7381 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     7441 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7501 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7561 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7621 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7681 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7741 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     7801 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7861 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7921 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     7981 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8041 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8101 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8161 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8221 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8281 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8341 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8401 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8461 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8521 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8581 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8641 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8701 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8761 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8821 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8881 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8941 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9001 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9061 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9121 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9181 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9241 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9301 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9361 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9421 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9481 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9541 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaa
//
