LOCUS       sequence-213510-       10508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Blast-rLAMP1-eGFP
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        189..1367
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          420..1358
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1315..1335
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1384..1402
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1445..1454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1447..1509,1510..2667)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     CDS             join(2692..2694,2695..2697,2698..3411)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2692..2694,2695..2697,2698..3408)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2737..2758)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2998..3017)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3345..3366
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3417..3441)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3472..4060
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3525..3545)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3943..3954)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4119..4139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4124..4453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4304..4439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4501..4548
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4567..4965
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5109..5342
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5414..5535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5451..5470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5505..5524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5575..5710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5731..5749)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5759..5776)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5759..5775)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5768..5790)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5917..6372
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6004..6023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6214..6235
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6398..6502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6503..6571,6572..7363)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6721..6740)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7534..8122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8023..8042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8276..8293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8410..8431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8446..8451,8452..8469,8470..8476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8484..8500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8489..8511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8508..8524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8508..8524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8543..8563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8545..8563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8591..8817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8818..8998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9045..9170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9663..9896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10081..10125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10392..10508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcgtccagt gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggctcc
       61 accatgggaa ccgacattga ttattgacta ggcttttgca aaaagctttg caaagatgga
      121 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
      181 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      241 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
      301 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      361 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      421 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      481 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
      541 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
      601 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
      661 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
      721 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
      781 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      841 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
      901 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
      961 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     1021 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     1081 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     1141 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     1201 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     1261 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     1321 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attagcttgg
     1381 tactaatacg actcactata gggagaccca agctggctag gtaagcttgg taccgagctc
     1441 ggatccatgg cggccccggg cgcccggcgg ccgctgctcc tgttgctgct ggcaggcctt
     1501 gcacacagcg ccccagcact gttcgaggtg aaagacaaca acggcacagc gtgtataatg
     1561 gccagcttct ctgcctcctt tctgaccacc tatgaggctg gacatgtttc taaggtctcg
     1621 aatatgaccc tgccagcctc tgcagaagtc ctgaagaata gcagctcttg tggtgaaaag
     1681 aatgcttctg agcccaccct cgcaatcacc tttggagaag gatatttact gaaactcacc
     1741 ttcacaaaaa acacaacacg ttacagtgtc cagcacatgt atttcacata taacctgtca
     1801 gacacacaat tctttcccaa tgccagctcc aaagggcccg acactgtgga ttccacaact
     1861 gacatcaagg cagacatcaa caaaacatac cgatgtgtca gcgacatcag ggtctacatg
     1921 aagaatgtga ccattgtgct ctgggacgct actatccagg cctacctgcc gagtagcaac
     1981 ttcagcaagg aagagacacg ctgcccacag gatcaacctt ccccaactac tgggccaccc
     2041 agcccctcac caccacttgt gcccacaaac cccagtgtgt ccaagtacaa tgtgactggt
     2101 gacaatggaa cctgcctgct ggcctctatg gcactgcaac tcaacatcac ctacatgaag
     2161 aaggacaaca cgactgtgac cagagcattc aacatcaacc caagtgacaa atatagtggg
     2221 acttgcggtg cccagttggt gaccctgaag gtggggaaca agagcagagt cctggagctg
     2281 cagtttggga tgaatgccac ttctagcctg tttttcctgc aaggagttca gttgaacatg
     2341 actcttcctg atgccataga gcccacgttc agcacctcca actattccct gaaagctctt
     2401 caggccagtg tcggcaactc atacaagtgc aacagtgagg agcacatctt tgtcagcaag
     2461 gcgctcgccc tcaatgtctt cagcgtgcaa gtccaggctt tcagggtaga aagtgacagg
     2521 tttgggtctg tggaagagtg tgtacaggac ggtaacaaca tgctgatccc cattgctgtg
     2581 ggcggggccc tggcagggct ggtcctcatt gtcctcatcg cctacctcat cggcaggaag
     2641 aggagtcacg cgggctatca gaccatcggt ggatctggag gttctggtgg aatggtgagc
     2701 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2761 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2821 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2881 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2941 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3001 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3061 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3121 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3181 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3241 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3301 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3361 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta gtctagaccc
     3421 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct
     3481 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3541 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3601 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3661 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3721 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3781 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3841 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     3901 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3961 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4021 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggacctgc aggattctgc
     4081 agatatccgg ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc agttagggtg
     4141 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4201 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4261 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4321 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4381 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4441 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     4501 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     4561 taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     4621 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     4681 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     4741 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     4801 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     4861 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     4921 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaaacgcg tcgcccgccc
     4981 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt
     5041 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5101 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     5161 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5221 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5281 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5341 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5401 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5461 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5521 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5581 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5641 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5701 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     5761 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5821 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5881 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     5941 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6001 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6061 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6121 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6181 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6241 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6301 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6361 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7561 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7621 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7681 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7741 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7801 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7861 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7921 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7981 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8041 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8101 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8161 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8221 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8281 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8341 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8401 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     8461 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8521 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     8581 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     8641 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     8701 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     8761 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     8821 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8881 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8941 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9001 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     9061 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     9121 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     9181 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     9241 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     9301 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     9361 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     9421 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9481 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     9541 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9601 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     9661 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9721 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     9781 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9841 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9901 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9961 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    10021 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    10081 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    10141 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    10201 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    10261 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    10321 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    10381 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10441 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc
    10501 aaaatttt
//
