LOCUS       sequence-213517-       10165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-On construct to induce expression of tandem mCherry-eGFP tagged 
            PMP34 (peroxisome marker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: PMP34-mCherry-EGFP-Tet-On
FEATURES             Location/Qualifiers
     source          1..10165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..368
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    62..80
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    98..116
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    133..151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    240..258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    276..294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     341..365
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1225..1932
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1370..1388)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1638..1657
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1960..1984
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1993..2002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1999..2001,2002..2004,2005..2718)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1999..2001,2002..2004,2005..2715)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2044..2065)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2305..2324)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2652..2673
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2746..3255
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3175..3193
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3265..4056
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3319..3338)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3929..3948
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             4066..4119
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4120..4737,4738..4743,4744..4779,4780..4782,4783..4818,
                     4819..4821,4822..4857,4858..4866)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4906..4926)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5324..5335)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5540..5773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5845..5966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5882..5901)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5936..5955
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6006..6141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6141..6212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6213..6281,6282..7073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6431..6450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7244..7832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7733..7752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7986..8003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8120..8141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8156..8161,8162..8179,8180..8186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8194..8210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8199..8221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8218..8234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8218..8234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8253..8273
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8255..8273
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8301..8527
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8528..8708
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8755..8880
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9373..9606
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9791..9835
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10048..10165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca ctttggccgc gaatcgatat gtcgagttta
       61 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
      121 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      181 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
      241 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
      301 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
      361 agatcgcctg gagaattggc tagcgccacc atgattgatg agaaaagaaa atccaaaact
      421 acacacatgg tgctcctgga gatcattaaa gaagaaggac tcctggcacc atatcgaggg
      481 tggtttccag tgatttccag tctctgctgc tccaattttg tctatttcta cacttttaat
      541 agcctcaaag cactctgggt caaaggtcaa cattctacca ctggaaaaga tctggtagtt
      601 gggtttgttg caggagtggt taatgtgttg ctaacaactc cactctgggt ggtaaacacc
      661 agactgaagc ttcaaggagc aaaatttagg aatgaagaca ttgtaccaac aaactacaaa
      721 ggtatcattg atgcttttca tcagatcatt cgcgatgaag gaatctcggc tttatggaat
      781 ggcacatttc cctcattgct gttggtcttc aatcctgcca tccagttcat gttttatgaa
      841 ggtttaaaac ggcagctttt aaagaaacgg atgaagcttt cttccttgga tgtgttcatc
      901 attggtgcag tagccaaagc gattgccacc acggtgacct atcccctgca gacggtacag
      961 tcaattctga ggtttgggcg tcatagacta aacccagaaa acagaacatt gggaagtctt
     1021 cggaatattc tctatcttct tcaccaacga gtaagacgtt ttggaataat gggactctac
     1081 aaaggccttg aagccaaact gctgcagaca gtcctcactg ctgctctcat gttccttgtt
     1141 tatgagaaac tgacagctgc caccttcaca gttatggggc tgaagcgtgc acaccaacac
     1201 ggtggatctg gaggttctgg tggaatggtg agcaagggcg aggaggataa catggccatc
     1261 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     1321 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1381 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1441 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     1501 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     1561 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1621 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1681 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1741 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1801 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1861 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1921 gagctgtaca agtccggact cagatccgct ggcccaggaa caagtttgta caaaaaagca
     1981 ggctttccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2161 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2221 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2461 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2581 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     2641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2701 gacgagctgt acaagtagtc cggactcaga tccggtaccg gatccggggt tggggttgcg
     2761 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     2821 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     2881 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     2941 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     3001 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     3061 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     3121 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     3181 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     3241 cgacctcttc cccagcaatt caccatgatt gaacaagatg gattgcacgc aggttctccg
     3301 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3361 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3421 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     3481 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     3541 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3601 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     3661 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     3721 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     3781 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     3841 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     3901 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     3961 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4021 cgcatcgcct tctatcgcct tcttgacgag ttcttccccg gtgccgaagg tagaggttct
     4081 ctcctcactt gtggtgatgt tgaagaaaac cctggtccaa tgtctagact ggacaagagc
     4141 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     4201 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     4261 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     4321 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     4381 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     4441 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     4501 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     4561 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     4621 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     4681 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     4741 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     4801 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     4861 gggtaactaa gtaaggatcg atccaagata tcgtattctt aactatgttg ctccttttac
     4921 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4981 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5041 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5101 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5161 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5221 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     5281 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5341 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5401 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc
     5461 ggactagagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5521 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5581 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5761 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5821 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5941 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6001 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6061 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6121 gaggaggctt ttttggaggc ctttcaaata tgtatccgct catgagacaa taaccctgat
     6181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6421 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6721 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8041 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8101 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8161 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8221 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     8281 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     8341 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     8401 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     8461 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     8521 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8701 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     8761 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8821 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8881 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8941 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     9001 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     9061 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     9121 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     9181 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     9241 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9301 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9361 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     9421 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9481 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9541 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9601 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9661 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9721 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9781 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9841 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9901 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9961 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacaat tctcgacctc
    10021 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
    10081 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
    10141 acaaattaca aaaattcaaa atttt
//
