LOCUS       sequence-213519-       11423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL1
FEATURES             Location/Qualifiers
     source          1..11423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    34..58
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        189..1367
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          420..1358
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1315..1335
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1384..1402
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1447..1449,1450..1452,1453..2163)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1447..1449,1450..1452,1453..2163)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1492..1513)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1753..1772)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2100..2121
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3870..3894)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3925..4513
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3978..3998)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4396..4407)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4564..5063
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4599..4622)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4996..5015
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5077..5880
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5140..5159)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5750..5769
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6024..6257
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6329..6450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6366..6385)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6420..6439
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6490..6625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6646..6664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6674..6691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6674..6690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6683..6705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6832..7287
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6919..6938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7129..7150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7313..7417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7418..7486,7487..8278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7636..7655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8449..9037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8938..8957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9191..9208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9325..9346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9361..9366,9367..9384,9385..9391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9399..9415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9404..9426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9423..9439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9423..9439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9458..9478
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9460..9478
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9506..9732
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9733..9913
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9960..10085
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10578..10811
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10996..11040
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11307..11423
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcgtccagt gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggctcc
       61 accatgggaa ccgacattga ttattgacta ggcttttgca aaaagctttg caaagatgga
      121 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
      181 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      241 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
      301 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      361 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      421 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      481 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
      541 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
      601 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
      661 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
      721 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
      781 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      841 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
      901 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
      961 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     1021 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     1081 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     1141 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     1201 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     1261 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     1321 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attagcttgg
     1381 tactaatacg actcactata gggagaccca agctggctag gtaagcttgg taccgagctc
     1441 agatccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1501 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1561 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1621 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1681 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1741 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1801 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1861 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1921 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1981 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2041 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2101 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2161 aagggtggat ctggaggttc tggcggaatg gccaagaacc gcagggacag aaacagttgg
     2221 ggtggatttt cggaaaagac atatgaatgg agctcagaag aggaggagcc agtgaaaaag
     2281 gcaggaccag tccaagtcct cattgtcaaa gatgaccatt cctttgagtt agatgaaact
     2341 gcattaaatc ggatccttct ctcggaggct gtcagagaca aggaggttgt tgctgtatct
     2401 gttgctggag catttagaaa aggaaaatca ttcctgatgg acttcatgtt gagatacatg
     2461 tacaaccagg aatcagttga ttgggttgga gactacaatg aaccattgac tggtttttca
     2521 tggagaggtg gatctgaacg agagaccaca ggaattcaga tatggagtga aatcttcctt
     2581 atcaataaac ctgatggtaa aaaggttgca gtgttattga tggatactca gggaaccttt
     2641 gatagtcagt caactttgag agattcagcc acagtatttg cccttagcac aatgatcagc
     2701 tcaatacagg tatataactt atcccaaaat gtccaggagg atgatcttca gcacctccag
     2761 cttttcactg agtatggcag actggcaatg gaggaaacat tcctgaagcc atttcagagt
     2821 ctgatatttc ttgttcgaga ctggagtttc ccatacgaat tttcatatgg agccgatggt
     2881 ggtgccaaat tcttggaaaa acgcctcaag gtctcaggga accagcatga agaactacag
     2941 aacgtcagaa aacacatcca ttcctgtttc accaacattt cctgttttct gctacctcat
     3001 cctggcttaa aagtagctac caatccaaac tttgatggaa aattgaaaga aatagatgat
     3061 gaattcatca aaaacttgaa aatactgatt ccttggctac ttagtcccga gagcctagat
     3121 attaaagaga tcaatgggaa taaaatcacc tgccggggtc tggtggagta cttcaaggct
     3181 tatataaaga tctatcaagg tgaagaatta ccacatccca aatccatgtt acaggccaca
     3241 gcagaagcta acaatttagc agccgtggca actgccaagg acacatacaa caaaaaaatg
     3301 gaagagattt gtggtggtga caaaccattt ctggccccaa atgacttgca gaccaaacac
     3361 ctgcaactta aggaagaatc tgtgaagcta ttccgagggg tgaagaagat gggtggggaa
     3421 gaatttagcc ggcgttacct gcagcagttg gagagtgaaa tagatgaact ttacatccaa
     3481 tatatcaagc acaatgatag caaaaatatc ttccatgcag ctcgtacccc agccacactg
     3541 tttgtagtca tctttatcac atatgtgatt gctggtgtga ctggattcat tggtttggac
     3601 atcatagcta gcctatgcaa tatgataatg ggactgaccc ttatcaccct gtgcacttgg
     3661 gcatatatcc ggtactctgg agaataccga gagctgggag ctgtaataga ccaggtggct
     3721 gcagctctgt gggaccaggg aagtacaaat gaggctttgt acaagcttta cagtgcagca
     3781 gcaacccaca gacatctgta tcatcaagct ttccctacac caaagtcgga atctactgaa
     3841 caatcagaaa agaaaaaaat gtaatctaga cccagctttc ttgtacaaag tggttgatat
     3901 ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3961 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4021 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4081 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4141 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4201 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4261 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     4321 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4381 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4441 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4501 gccgcctccc cgcctggacc tgcaggattc tgcagatatc cggactagtg atctaattct
     4561 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     4621 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     4681 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     4741 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     4801 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     4861 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     4921 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     4981 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     5041 cttcctcatc tccgggcctt tcgacctgca gccaatatgg gatcggccat tgaacaagat
     5101 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5161 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5221 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5281 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5341 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5401 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5461 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5521 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5581 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5641 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5701 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5761 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5821 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5881 acgcgtcgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     5941 tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6001 ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct
     6061 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6121 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6181 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6241 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6301 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6361 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6421 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6481 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6541 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6601 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     6661 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     6721 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     6781 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     6841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6961 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7021 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7081 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7141 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7201 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7261 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     7321 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7381 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7441 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7501 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7561 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7621 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7681 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7741 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7801 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7861 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7921 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7981 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8041 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8101 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8161 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8221 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8281 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8341 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8401 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8461 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8521 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8581 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8641 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8701 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8761 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8821 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8881 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8941 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9001 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9061 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9121 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9181 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9241 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9301 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9361 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9421 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     9481 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     9541 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     9601 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     9661 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     9721 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9781 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9841 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9901 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     9961 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10021 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10081 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10141 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10201 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10261 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10321 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10381 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    10441 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10501 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10561 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10621 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10681 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10741 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10801 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10861 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10921 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    10981 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    11041 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    11101 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    11161 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    11221 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    11281 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
    11341 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    11401 caaattacaa aattcaaaat ttt
//
