LOCUS       sequence-21352-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Shaw AT, Meissner A, Dowdle JA, Crowley D, Magendantz M, Ouyang C, 
            Parisi T, Rajagopal J, Blank LJ, Bronson RT, Stone JR, Tuveson DA, 
            Jaenisch R, Jacks T
  TITLE     Sprouty-2 regulates oncogenic K-ras in lung development and 
            tumorigenesis.
  JOURNAL   Genes Dev. 2007 Mar 15. 21(6):694-707.
  PUBMED    17369402
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2007 Mar 15. 21(6):694-707."
COMMENT     Sequence Label: pSico Spry-2
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(11..44)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     111..127
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    202..790
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(255..275)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(673..684)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(793..809)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcggccgc ataacttcgt atagtataaa ttatacgaag ttataagcct tgtttgccgg
       61 gttgtcgttg taaattcaag agatttacaa cgacaacccg gcttttttct cgaggtcgac
      121 ggtatcgata agctcgcttc acgagatcat gtttaagggt tccggttcca ctaggtacaa
      181 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
      241 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
      301 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
      361 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
      421 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
      481 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
      541 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
      601 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
      661 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
      721 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
      781 gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac
      841 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctaga
//
