LOCUS       sequence-213527-       11321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL3
FEATURES             Location/Qualifiers
     source          1..11321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      123..711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     612..631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     865..882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    999..1020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1035..1040,1041..1058,1059..1065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1073..1089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1078..1100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1097..1113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1097..1113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1132..1152
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1134..1152
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1180..1406
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1407..1587
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1634..1759
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2252..2485
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2670..2714
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2981..3097
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3131..3155
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3286..4464
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3517..4455
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4412..4432
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4481..4499
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4544..4546,4547..4549,4550..5260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4544..4546,4547..4549,4550..5260)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4589..4610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4850..4869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5197..5218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6865..6889)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6920..7508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6973..6993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7391..7402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7559..8058
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7594..7617)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7991..8010
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8072..8875
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8135..8154)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8745..8764
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9019..9252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9324..9445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9361..9380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9415..9434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9485..9620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9641..9659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9669..9686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9669..9685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9678..9700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9827..10282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9914..9933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10124..10145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10308..10412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10413..10481,10482..11273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10631..10650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
       61 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
      121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
      181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
      241 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
      301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
      361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      841 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      901 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      961 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     1021 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     1081 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     1141 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     1201 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     1261 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     1321 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     1381 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     1441 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     1501 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     1561 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     1621 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     1681 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     1741 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     1801 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1861 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1921 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1981 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     2041 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     2101 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     2161 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     2221 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     2281 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     2341 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     2401 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     2461 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     2521 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     2581 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2641 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2701 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2761 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2821 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2881 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2941 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
     3001 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     3061 aagaattaca aaaacaaatt acaaaattca aaattttatc gtccagtgtg gtggaattct
     3121 gcagatatca acaagtttgt acaaaaaagc aggctccacc atgggaaccg acattgatta
     3181 ttgactaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
     3241 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     3301 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     3361 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3421 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3481 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     3541 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     3601 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3661 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3721 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3781 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3841 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3901 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3961 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     4021 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     4081 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     4141 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     4201 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     4261 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     4321 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     4381 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     4441 tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactataggg
     4501 agacccaagc tggctaggta agcttggtac cgagctcgga tccatggtga gcaagggcga
     4561 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4621 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4681 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4741 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4801 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4861 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4921 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4981 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5041 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5101 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5161 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5221 cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggatctg gaggttctgg
     5281 tggaatggag agcagcaagc ctggtccagt gcaggttgtt ttggttcaga aagatcaaca
     5341 ttcctttgag ctagatgaga aagccttggc cagcatcctc ttgcaggacc acatccgaga
     5401 tcttgatgtg gtggtggttt cagtggctgg tgccttccga aagggcaagt ccttcattct
     5461 ggattttatg ctacgatact tatattctca gaaggaaagt ggccattcaa attggttggg
     5521 tgacccagaa gaaccgttaa caggattttc ctggagaggg ggatctgatc cagaaaccac
     5581 tgggattcaa atctggagtg aagttttcac tgtggagaag ccaggtggga agaaggttgc
     5641 agttgttctg atggataccc agggggcatt tgacagccag tcaactgtga aagactgtgc
     5701 taccatcttt gctctaagca ctatgactag ttctgttcag atttataatt tatctcagaa
     5761 cattcaagaa gatgatcttc aacagctgca gctcttcaca gaatacggtc gtctggcaat
     5821 ggatgaaatt ttccaaaagc ctttccagac actgatgttt ttggttagag attggagttt
     5881 cccttatgaa tatagctatg gactccaagg aggaatggca tttttggata agcgtttaca
     5941 ggtgaaggaa catcaacatg aagaaattca gaatgttcga aatcacattc actcatgttt
     6001 ctccgatgtc acctgctttc tcttaccaca tccaggactc caggtggcca caagccctga
     6061 ctttgatggg aaattaaaag atattgctgg tgaattcaaa gagcagttac aggcactgat
     6121 accgtatgta ttaaacccat ctaagttaat ggaaaaggag atcaatggct caaaggtcac
     6181 ctgtcgggga ctactggagt attttaaggc atatattaaa atttatcaag gagaagatct
     6241 gcctcacccc aagtccatgc ttcaggccac tgctgaagcc aacaacttag cagctgcagc
     6301 ctctgccaag gacatttatt ataacaacat ggaagaggtt tgtgggggag agaaacctta
     6361 tttgtctcca gacattctag aggagaagca ctgtgaattc aaacaacttg ctctggacca
     6421 ttttaagaag accaagaaga tgggtgggaa ggatttcagc tttcgttacc agcaggagct
     6481 ggaggaggaa atcaaggaat tatatgagaa cttctgcaag cacaatggta gcaagaacgt
     6541 cttcagcacc ttccgaaccc ctgcagtgct gttcacgggc attgtagctt tgtacatagc
     6601 ctcaggcctc actggcttca taggtcttga ggttgtagcc cagttgttca actgtatggt
     6661 tggactactg ttaatagcac tcctcacctg gggctacatc aggtattctg gtcaatatcg
     6721 tgagctgggc ggagctattg attttggtgc cgcatatgtg ttggagcagg cttcttctca
     6781 tatcggtaat tccactcagg ccactgtgag ggatgcagtt gttggaagac catccatgga
     6841 taaaaaagct caataatagt ctagacccag ctttcttgta caaagtggtt gatatccagc
     6901 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6961 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7021 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7081 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7141 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7201 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7261 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7321 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7381 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7441 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7501 ctccccgcct ggacctgcag gattctgcag atatccggac tagtgatcta attctaccgg
     7561 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     7621 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     7681 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     7741 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     7801 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     7861 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     7921 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     7981 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     8041 tcatctccgg gcctttcgac ctgcagccaa tatgggatcg gccattgaac aagatggatt
     8101 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     8161 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     8221 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     8281 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     8341 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     8401 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     8461 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     8521 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     8581 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     8641 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     8701 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     8761 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8821 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaacgcg
     8881 tcgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg
     8941 agggcccggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9001 taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt
     9061 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9241 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9301 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     9361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9421 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     9481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     9541 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     9601 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     9661 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     9721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     9781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     9841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    10081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    10201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10261 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    10681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    10801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    10861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    10921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    10981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11281 accaagttta ctcatatata ctttagattg atttaaaact t
//
