LOCUS       sequence-213530-        9944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescently labels WT human YAP1-2gamma isoform (504 aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Ege N, Dowbaj AM, Jiang M, Howell M, Hooper S, Foster C, Jenkins RP,
            Sahai E
  TITLE     Quantitative Analysis Reveals that Actin and Src-Family Kinases 
            Regulate Nuclear YAP1 and Its Export.
  JOURNAL   Cell Syst. 2018 Jun 27;6(6):692-708.e13. doi: 
            10.1016/j.cels.2018.05.006. Epub 2018 Jun 18.
  PUBMED    29909276
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2018.05.006"; journalName: "Cell Syst"; date: 
            "2018-06-27- 27"; volume: "6"; issue: "6"; pages: "692-708.e13"
COMMENT     Sequence Label: pLL3.7-EF-EYFP-YAP1_WT-PolyA
FEATURES             Location/Qualifiers
     source          1..9944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1865..1909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2398..2414
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2456..2489)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2553..3731
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2784..3722
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3679..3699
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3811..3820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3817..3819,3820..3822,3823..4533)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3862..3883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4123..4142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4470..4491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4543..4567
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6083..6107)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6368..6449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6405..6424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6459..6478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(6507..6540)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6596..7184
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6649..6669)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7067..7078)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7187..7203)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7713..7893
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7955..8543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8035..8054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8714..9505,9506..9574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9337..9356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9575..9679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9754..9773)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     1861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     1921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     1981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2161 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gaacgcgcgg tgaccctcga
     2401 ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg acctaataac
     2461 ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag cggccggccg
     2521 cgtggataac cgtattaccg ccataatcgt gaggctccgg tgcccgtcag tgggcagagc
     2581 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     2641 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2701 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2761 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     2821 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     2881 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     2941 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     3001 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3061 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3121 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3181 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3241 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3301 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3361 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3421 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3481 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     3541 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     3601 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     3661 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     3721 aggtgtcgtg aggaattagc ttgcttacat ttgcttctga cacaactgtg ttcactagca
     3781 acctcaaaca gacagctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3901 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     4021 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4081 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4141 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4201 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4261 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     4321 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4381 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     4441 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4501 gggatcactc tcggcatgga cgagctgtac aagtccggat caacaagttt gtacaaaaaa
     4561 gcaggcttgg atcccgggca gcagccgccg cctcaaccgg ccccccaggg ccaagggcag
     4621 ccgccttcgc agcccccgca ggggcagggc ccgccgtccg gacccgggca accggcaccc
     4681 gcggcgaccc aggcggcgcc gcaggcaccc cccgccgggc atcagatcgt gcacgtccgc
     4741 ggggactcgg agaccgacct ggaggcgctc ttcaacgccg tcatgaaccc caagacggcc
     4801 aacgtgcccc agaccgtgcc catgaggctc cggaagctgc ccgactcctt cttcaagccg
     4861 ccggagccca aatcccactc ccgacaggcc agtactgatg caggcactgc aggagccctg
     4921 actccacagc atgttcgagc tcattcctct ccagcttctc tgcagttggg agctgtttct
     4981 cctgggacac tgacccccac tggagtagtc tctggcccag cagctacacc cacagctcag
     5041 catcttcgac agtcttcttt tgagatacct gatgatgtac ctctgccagc aggttgggag
     5101 atggcaaaga catcttctgg tcagagatac ttcttaaatc acatcgatca gacaacaaca
     5161 tggcaggacc ccaggaaggc catgctgtcc cagatgaacg tcacagcccc caccagtcca
     5221 ccagtgcagc agaatatgat gaactcggct tcaggtcctc ttcctgatgg atgggaacaa
     5281 gccatgactc aggatggaga aatttactat ataaaccata agaacaagac cacctcttgg
     5341 ctagacccaa ggcttgaccc tcgttttgcc atgaaccaga gaatcagtca gagtgctcca
     5401 gtgaaacagc caccacccct ggctccccag agcccacagg gaggcgtcat gggtggcagc
     5461 aactccaacc agcagcaaca gatgcgactg cagcaactgc agatggagaa ggagaggctg
     5521 cggctgaaac agcaagaact gcttcggcag gcaatgcgga atatcaatcc cagcacagca
     5581 aattctccaa aatgtcagga gttagccctg cgtagccagt taccaacact ggagcaggat
     5641 ggtgggactc aaaatccagt gtcttctccc gggatgtctc aggaattgag aacaatgacg
     5701 accaatagct cagatccttt ccttaacagt ggcacctatc actctcgaga tgagagtaca
     5761 gacagtggac taagcatgag cagctacagt gtccctcgaa ccccagatga cttcctgaac
     5821 agtgtggatg agatggatac aggtgatact atcaaccaaa gcaccctgcc ctcacagcag
     5881 aaccgtttcc cagactacct tgaagccatt cctgggacaa atgtggacct tggaacactg
     5941 gaaggagatg gaatgaacat agaaggagag gagctgatgc caagtctgca ggaagctttg
     6001 agttctgaca tccttaatga catggagtct gttttggctg ccaccaagct agataaagaa
     6061 agctttctta catggttata ggacccagct ttcttgtaca aagtggttga tccaccggat
     6121 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     6181 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttggcc
     6241 gcggatccgt ttaaacgaat tcatcataat cagccatacc acatttgtag aggttttact
     6301 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     6361 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6421 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6481 tgtatcccaa ttcgtcgagg gacctaataa cttcgtatag catacattat acgaagttat
     6541 acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca
     6601 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6661 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6721 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6781 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6841 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6901 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6961 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7021 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7081 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7141 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     7201 tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     7261 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     7321 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7381 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     7441 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     7501 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     7561 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     7621 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     7681 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     7741 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7801 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7861 gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg
     7921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9721 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9781 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     9841 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     9901 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
