LOCUS       sequence-213533-        4098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector containing CDS for the modified human heavy chain of 
            the model chimeric mouse-human IgG1 expressed by the Agarabi CHO 
            cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Velugula-Yellela SR, Williams A, Trunfio N, Hsu CJ, Chavez B, Yoon 
            S, Agarabi C
  TITLE     Impact of media and antifoam selection on monoclonal antibody 
            production and quality using a high throughput micro-bioreactor 
            system.
  JOURNAL   Biotechnol Prog. 2018 Jan;34(1):262-270. doi: 10.1002/btpr.2575. 
            Epub 2017 Nov 16.
  PUBMED    29086492
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/btpr.2575";
            journalName: "Biotechnol Prog"; date: "2018-01"; volume: "34"; 
            issue: "1"; pages: "262-270"
COMMENT     Sequence Label: Agarabi CHO Heavy Chain in pJ201
FEATURES             Location/Qualifiers
     source          1..4098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1718..1737)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        1804..1895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1896..2705
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2827..3415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3316..3335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3569..3586
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3684..3701
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3685..3701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actataagat gttatcagta tttattatgc atttagaata aattttgtgt cgccctttac
       61 acgtacttag tcgctgaaaa gctggctagc gccgccacca tgaaatgggt cacctttatc
      121 tccctgctgt ttctcttctc ctcggcctat tcacaagtgc agctccagca gcccggcgcc
      181 gagcttgtga agccgggagc gtccgtcaag atgtcttgta aagccagcgg ttacaccttc
      241 acctcctaca acatgcattg ggtcaagcag acacctggac agggactgga atggattggc
      301 gcgatctatc cgggcaacgg agacacttcc tacaatcaaa agttcaaggg gaaggccacc
      361 ctgaccgccg acaagagcag cagcaccgcc tacatgcagc tctcctcgct gacctccgag
      421 gattcggccg tgtactactg cgcccggagc cgcgccaggg cccgcgaagc aatggattac
      481 tggggacagg ggacctctgt cactgtgtcc agcgcctcca ccaagggacc ttcagtgttc
      541 ccgctggcgc ccagctccaa gtccacttcc ggtggcaccg ctgccctggg ttgcctcgtc
      601 aaggactatt tccccgaacc tgtgaccgtg tcctggaact ccggcgcgct cacttccggc
      661 gtgcacacat tccctgctgt gctccagtca agcggactct actcgctttc gagcgtggtc
      721 actgtgcctt cgtcctccct ggggactcag acctacattt gcaacgtgaa ccacaagcca
      781 tccaacacca aagtggataa gaaggtcgaa ccgaagtcct gtgacaagac ccacacgtgc
      841 ccgccttgcc cggcccccga acttttggga gggcccagcg tgttcctgtt cccgccaaaa
      901 ccaaaggata cgctgatgat cagccggacc cccgaagtca cttgtgtggt ggtcgatgtg
      961 tcgcatgagg accctgaagt caagttcaat tggtacgtgg acggcgtgga ggtgcacaac
     1021 gccaagacca agccgcggga ggaacagtac aactccactt accgcgtggt gtcagtgctg
     1081 accgtgctgc accaagactg gctgaacggg aaggagtaca agtgcaaagt gtccaacaag
     1141 gctttgccag cgccgattga aaagaccatc agcaaggcca agggacagcc gagagaaccc
     1201 caggtgtaca ctctgccgcc ttcacgggac gagctgacca agaaccaagt gtcgctgact
     1261 tgcctcgtga agggtttcta cccgtccgac atcgcagtgg aatgggagtc gaacggccaa
     1321 cccgagaaca actacaagac cacccctccc gtcctggact ccgacggatc cttcttcctg
     1381 tactccaagc tgaccgtgga caagtcgaga tggcagcagg gcaacgtgtt ttcctgctcc
     1441 gtgatgcacg aggcactgca taatcactac actcagaagt cactgtcatt gagccccgga
     1501 aagtgacgtc aatcgagttc gtacctaagg gcgacacccc ctaattagcc cgggcgaaag
     1561 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
     1621 atggggagtc cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg
     1681 gtcaggtggg accaccgcgc tactgccgcc aggcaaacaa ggggtgttat gagccatatt
     1741 caggtataaa tgggctcgcg ataatgttca gaattggtta attggttgta acactgaccc
     1801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1861 gataaatgct tcaataatat tgaaaaagga agaatatgag ccatattcaa cgggaaacgt
     1921 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     1981 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     2041 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     2101 gactaaactg gctgacggaa tttatgccac ttccgaccat caagcatttt atccgtactc
     2161 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcgttc caggtattag
     2221 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     2281 tgcactcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgcctcgctc
     2341 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     2401 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     2461 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     2521 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     2581 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     2641 atggtattga taatcctgat atgaataaat tgcaatttca tttgatgctc gatgagtttt
     2701 tctaagcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2761 aacgtgagtt acgcgcgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2941 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttagccca
     3001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3481 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3541 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga
     3601 gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc
     3661 gtttctacaa actctttctg tgttgtaaaa cgacggccag tcttaagctc gggccccctg
     3721 ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg
     3781 gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa gggcgcctgt
     3841 cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag caacgcactt
     3901 taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta ttcatctatt
     3961 atttatgatt ttttgtatat acaatatttc tagtttgtta aagagaatta agaaaataaa
     4021 tctcgaaaat aataaaggga aaatcagttt ttgatatcaa aattatacat gtcaacgata
     4081 atacaaaata taatacaa
//
