LOCUS       sequence-213535-        3387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector containing CDS for the murine light chain of the 
            model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell 
            line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Velugula-Yellela SR, Williams A, Trunfio N, Hsu CJ, Chavez B, Yoon 
            S, Agarabi C
  TITLE     Impact of media and antifoam selection on monoclonal antibody 
            production and quality using a high throughput micro-bioreactor 
            system.
  JOURNAL   Biotechnol Prog. 2018 Jan;34(1):262-270. doi: 10.1002/btpr.2575. 
            Epub 2017 Nov 16.
  PUBMED    29086492
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/btpr.2575";
            journalName: "Biotechnol Prog"; date: "2018-01"; volume: "34"; 
            issue: "1"; pages: "262-270"
COMMENT     Sequence Label: Agarabi CHO Light Chain in pJ201
FEATURES             Location/Qualifiers
     source          1..3387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..85
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1489..1506)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1489..1505)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1604..1621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1775..2363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1855..1874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2485..3294)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(3295..3386)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc catttatacc
       61 tgaatatggc tcataacacc ccttgtttgc ctggcggcag tagcgcggtg gtcccacctg
      121 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggactcccc
      181 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      241 gcctttcgcc cgggctaatt agggggtgtc gccctttaca cgtacttagt cgctgaaaag
      301 ctggctagcg ccgccaccat gaaatgggtc acttttatct cactgctttt cttgttctcc
      361 tccgcctact cccaaattgt gctcacccaa tcccctgcca tcatgtccgc ctcccccggc
      421 gaaaaggtca ccatgacttg ttccgcctca tcgtccgtgt cctacatgca ttggtaccag
      481 cagaagtcag gcacctcacc aaagagatgg atctacgaca catcgaagct ggcctccggc
      541 gtgcctgcgc ggttctcggg atcgggaagc gggaccagct actccctgac tatctcgtcc
      601 atggaagccg aggacgctgc aacctattac tgccaacagt ggtccagcaa ccccttcacc
      661 ttcgggtctg gcaccaagct ggagattaag aggaccgtgg cggcgccctc cgtgtttatc
      721 ttcccgccga gcgatgagca gctcaagagc ggaactgcaa gcgtggtctg cctgctcaac
      781 aatttctacc cgcgcgaagc caaagtgcag tggaaggtcg acaacgctct gcagtccgga
      841 aacagccagg aatcggtgac cgaacaggat tccaaggaca gcacctactc cctctcatcg
      901 actctgactc tgagcaaggc cgactacgag aagcacaagg tctacgcctg cgaagtgacc
      961 caccagggtc tgtccagccc tgtgacgaag tccttcaacc ggggagagtg ctagcgtcaa
     1021 tcgagttcgt acctaagggc gacacaaaat ttattctaaa tgcataataa atactgataa
     1081 catcttatag tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa
     1141 aactgatttt ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag
     1201 aaatattgta tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca
     1261 tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt
     1321 taaataattc tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct
     1381 ctttccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg
     1441 attaacgatt actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt
     1501 tttacaacac agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc
     1561 ttagtttgat gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc
     1621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2161 taggcggtgc tacagagttc ttgaagtggt gggctaacta cggctacact agaagaacag
     2221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2401 agtggaacga cgcgcgcgta actcacgtta agggattttg gtcatgagct tgcgccgtcc
     2461 cgtcaagtca gcgtaatgct ctgcttagaa aaactcatcg agcatcaaat gaaattgcaa
     2521 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     2581 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     2641 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     2701 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     2761 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     2821 caaaccgtta ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa
     2881 aggacaatta caaacaggaa tcgagtgcaa ccggcgcagg aacactgcca gcgcatcaac
     2941 aatattttca cctgaatcag gatattcttc taatacctgg aacgctgttt ttccggggat
     3001 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     3061 tggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     3121 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaagcgata
     3181 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     3241 atccatgttg gaatttaatc gcggcctcga cgtttcccgt tgaatatggc tcatattctt
     3301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3361 tgaatgtatt tagaaaaata aacaaat
//
