LOCUS       sequence-213565-        7944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 1-910).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 1-910
FEATURES             Location/Qualifiers
     source          1..7944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3327..3347)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3327..3345)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3366..3382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3366..3382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3379..3401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3390..3406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3414..3420,3421..3438,3439..3444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3459..3480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3597..3614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3768..4356)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3848..3867)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4527..5318,5319..5387))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5150..5169
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5388..5492)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5529..6032
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     6073..6091
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6129..6151)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6251..6270
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6291..6571
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6572..7246
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7349..7804)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7486..7507)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7698..7717
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7930..7944,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7944,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgag tcatccgatt caatttgtaa acgctaataa
      361 tagcgataag agccaccaat taggcggtca atattctatt cctcaggatt tgagggaaaa
      421 cctgcagaaa gaagctgcac gtattggtga gaacgaaaaa gatgtgctac aggaaaaaat
      481 ggaaactagg acagtgcaaa atcgagagga ttcataccac aaaagaaggt ttgatatgaa
      541 gtttgagccg gattccgata cacaaactgt tacgtcttca gaaaataccc aagacgctgt
      601 cgtgccaagg aaaagaaaat ctcgttggga tgtaaaaggg tacgagccac ctgatgaatc
      661 ttcaacagcg gtcaaagaaa actctgatag tgctttggtt aatgtagaag gaatccatga
      721 tttgatgttt tttaaacctt ctgaccataa atatttcgcc gacgttatat ccaaaaaacc
      781 catagatgag ttaaataagg atgaaaagaa agaaagaacc ctgtcgatgt tacttctgaa
      841 aataaaaaat ggcaatacag cgagtaggag gacttctatg cggattttaa ccgataaagc
      901 tgttacattt ggtccagaaa tgatatttaa ccgcttattg cccatattac tagacagaag
      961 tttggaagac caggaaagac atctaatgat caagaccata gatcgcgtcc tttaccagct
     1021 aggagattta acaaaacctt acgttcacaa aatacttgta gtagctgccc cattgctaat
     1081 agatgaagat ccaatggttc gttcaacagg ccaggagata atcaccaatc tgtctacagt
     1141 cgctggccta aaaaccattc taacagtaat gagacctgat attgagaatg aggatgagta
     1201 tgttagaaat gtaacttcaa gagcggcggc tgtggtggca aaagcccttg gtgttaatca
     1261 attgcttcct ttcatcaatg cagcatgcca ctcacggaag tcttggaaag caaggcatac
     1321 tggcataaaa attgtgcagc aaataggtat acttttggga ataggtgtct tgaatcatct
     1381 tactgggctg atgagttgta tcaaagactg cctaatggat gatcacgttc cggtgcgaat
     1441 tgttacagca catactttat caacactagc agaaaattct tatccatatg gtatcgaagt
     1501 tttcaacgtt gtactggagc cattatggaa gggtataaga agtcatcgtg gtaaagtgct
     1561 atcttctttt ttaaaagctg ttggatctat gatcccattg atggacccag agtatgccgg
     1621 ttattatacc acagaagcaa tgagaataat tagacgtgaa ttcgactctc ccgatgatga
     1681 aatgaaaaag accattcttt tagtattaca aaaatgtagc gctgtagaat ccataacgcc
     1741 aaaatttctg agagaagaaa tcgcgccaga attctttcaa aaattttggg tcagacgagt
     1801 ggcattggat agaccattaa acaaagttgt tacatatact actgttacat tagcaaagaa
     1861 attaggttgt tcttatacca ttgataaatt attaacacca ttgagagatg aagctgagcc
     1921 gttcagaaca atggctgtcc atgctgttac tagaacagta aacttattag ggacagcaga
     1981 tttagatgag agattagaaa cgaggcttat tgatgcgctc ttgatagcat ttcaagaaca
     2041 aacaaatagt gattctataa tctttaaagg gtttggggca gtgacggtgt cacttgacat
     2101 ccgtatgaaa ccgtttttag cacccattgt gagtacgatt ttaaatcatt taaagcataa
     2161 aactcccttg gttcgtcaac atgcagcaga tctatgtgcc atactaatac cagtcatcaa
     2221 gaactgtcac gaattcgaaa tgttgaataa attgaacatt atattgtatg aatccttagg
     2281 cgaagtatat ccagaggtat taggttctat aatcaacgct atgtactgta ttactagcgt
     2341 aatggatttg gataaactac aacctcctat taatcaaatt ttaccaacct taactccaat
     2401 tttaagaaac aagcatagaa aagtcgaagt aaacactatc aaatttgttg gacttattgg
     2461 taaattagca cctacctatg cacctcctaa agaatggatg agaatatgtt ttgaactatt
     2521 agaacttttg aaaagtacaa ataaagaaat aagaagatca gcaaatgcca cgtttggatt
     2581 cattgctgaa gcaattggac ctcacgatgt tcttgttgcg ttattgaaca atttaaaggt
     2641 tcaagaacgt cagttacgtg tatgcacggc tgttgctatc ggtatcgttg ctaaagtgtg
     2701 tggtccttac aacgtgctgc cagtaatcat gaatgaatac acaacaccag agactaatgt
     2761 tcaaaacggt gttctcaagg ccatgtcatt catgtttgag tacattggta acatgtctaa
     2821 ggattacata tacttcataa caccattatt agaagatgca ctcaccgata gggatttagt
     2881 tcatcgtcag acagcgtcga acgtaataac ccacttagct ttaaactgtt caggtacagg
     2941 tcacgaagat gccttcattc atttaatgaa tcttctaata cccaatattt ttgaaacttc
     3001 accacatgct attatgcgta ttttagaagg tttggaggca ctgagtcaag ctctttgaat
     3061 atatatagag catataaata accacaactt gtaagaatat tttacttgac acacctatat
     3121 catcacgtca cgatgccttc atagattatg atgaaatata aacagaacct gctattggaa
     3181 aaaaaagggc aggaaaaaat tttgtgaaag tgagtaaaag aaagatgaag agcgagacat
     3241 ggataataag aaatagatgt cactaatgga caattggaag actgatatgg aaagttacga
     3301 tgaaggagga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     3361 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3421 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3481 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3541 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3601 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3661 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3721 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3781 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3841 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3901 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3961 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4021 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4081 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4141 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4201 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4261 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4321 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4381 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4441 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4501 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4561 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4621 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4681 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4741 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4801 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4861 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4921 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4981 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5041 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5101 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5161 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5221 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5281 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5341 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5401 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5461 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
     5521 tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata
     5581 aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga
     5641 tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc
     5701 tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc
     5761 tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt
     5821 gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat
     5881 ttttttttct tcattccgta actcttctac cttctttatt tactttctaa aatccaaata
     5941 caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat
     6001 acgaggcgcg tgtaagttac aggcaagcga tccgtctaag aaaccattat tatcatgaca
     6061 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     6121 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6181 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     6241 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatagatc acgacattac
     6301 tatatatata atataggaag catttaatag aacagcatcg taatatatgt gtactttgca
     6361 gttatgacgc cagatggcag tagtggaaga tattctttat tgaaaaatag cttgtcacct
     6421 tacgtacaat cttgatccgg agcttttctt tttttgccga ttaagaatta attcggtcga
     6481 aaaaagaaaa ggagagggcc aagagggagg gcattggtga ctattgagca cgtgagtata
     6541 cgtgattaag cacacaaagg cagcttggag tatgtctgtt attaatttca caggtagttc
     6601 tggtccattg gtgaaagttt gcggcttgca gagcacagag gccgcagaat gtgctctaga
     6661 ttccgatgct gacttgctgg gtattatatg tgtgcccaat agaaagagaa caattgaccc
     6721 ggttattgca aggaaaattt caagtcttgt aaaagcatat aaaaatagtt caggcactcc
     6781 gaaatacttg gttggcgtgt ttcgtaatca acctaaggag gatgttttgg ctctggtcaa
     6841 tgattacggc attgatatcg tccaactgca tggagatgag tcgtggcaag aataccaaga
     6901 gttcctcggt ttgccagtta ttaaaagact cgtatttcca aaagactgca acatactact
     6961 cagtgcagct tcacagaaac ctcattcgtt tattcccttg tttgattcag aagcaggtgg
     7021 gacaggtgaa cttttggatt ggaactcgat ttctgactgg gttggaaggc aagagagccc
     7081 cgaaagctta cattttatgt tagctggtgg actgacgcca gaaaatgttg gtgatgcgct
     7141 tagattaaat ggcgttattg gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa
     7201 agactctaac aaaatagcaa atttcgtcaa aaatgctaag aaataggtta ttactgagta
     7261 gtatttattt aagtattgtt tgtgcacttg ccgatctatg cggtgtgaaa taccgcacag
     7321 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
     7381 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     7441 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     7501 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     7561 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     7621 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     7681 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     7741 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     7801 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7861 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7921 cagggttttc ccagtcacga cgtt
//
