LOCUS       sequence-213580-        6425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid containing Cre-recombinase gene, which is expressed 
            in DH10MultiBac cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Berger I, Fitzgerald DJ, Richmond TJ
  TITLE     Baculovirus expression system for heterologous multiprotein 
            complexes.
  JOURNAL   Nat Biotechnol. 2004 Dec;22(12):1583-7. doi: 10.1038/nbt1036. Epub 
            2004 Nov 28.
  PUBMED    15568020
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt1036"; 
            journalName: "Nat Biotechnol"; date: "2004-12"; volume: "22"; issue:
            "12"; pages: "1583-7"
COMMENT     Sequence Label: pBADZ-HisCre
FEATURES             Location/Qualifiers
     source          1..6425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..82
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(262..1140)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1167..1451
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1374..1393
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1492..1509
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1510..2541
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     primer_bind     complement(2669..2686)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2839..2925
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3017..3044
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3063..3154
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3373..3392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3525..3899)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3918..3965)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(3972..4385)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     rep_origin      4837..5292
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4924..4943)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5134..5155
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5403..5991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5892..5911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6145..6162
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6328..6350
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
       61 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
      121 acgatgcgtc cggcgtagag gatctgctca tgtttgacag cttatcatcg atgcataatg
      181 tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc
      241 aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc
      301 acaaccggca cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa
      361 atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt
      421 gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat
      481 ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc
      541 gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc
      601 gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag
      661 taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc
      721 ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg
      781 aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg
      841 cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg
      901 atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc
      961 ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc
     1021 cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc
     1081 caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat
     1141 acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg
     1201 ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa
     1261 aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta
     1321 taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat
     1381 agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac
     1441 tgtttctcca tacccgtttt tttgggctag caggaggaat tcaccatggc acatcatcat
     1501 caccaccaca tgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
     1561 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
     1621 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
     1681 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
     1741 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
     1801 cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg
     1861 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
     1921 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat
     1981 atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
     2041 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
     2101 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
     2161 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
     2221 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
     2281 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
     2341 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
     2401 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
     2461 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
     2521 ctgctggaag atggcgatta gccattaacg cgtaaatgat tgctataatt agttgatatt
     2581 atctagagct gggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct
     2641 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
     2701 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
     2761 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
     2821 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2881 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     2941 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     3001 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact
     3061 cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3121 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3181 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3241 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3301 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3361 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     3421 cggtcgccgc atacactatt ctcagaatga cttggttgag atcgacaaag gaaaaggggg
     3481 acggatctcc gaggcctcgg acccgtcggg ccgcgtcgga cgtgtcagtc ctgctcctcg
     3541 gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg cccccacggc
     3601 tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc
     3661 tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg
     3721 tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca
     3781 ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt ccagaactcg
     3841 accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa cttggccatg
     3901 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     3961 tcaacaccgc ccttagatta gattgctatg ctttctttct aatgagcaag aagtaaaaaa
     4021 agttgtaata gaacaagaaa aatgaaactg aaacttgaga aattgaagac cgtttattaa
     4081 cttaaatatc aatgggaggt catcgaaaga gaaaaaaatc aaaaaaaaaa attttcaaga
     4141 aaaagaaacg tgataaaaat ttttattgcc tttttcgacg aagaaaaaga aacgaggcgg
     4201 tctctttttt cttttccaaa cctttagtac gggtaattaa cgacacccta gaggaagaaa
     4261 gaggggaaat ttagtatgct gtgcttgggt gttttgaagt ggtacggcga tgcgcggagt
     4321 ccgagaaaat ctggaagagt aaaaaaggag tagaaacatt ttgaagctat ggtgtgtggg
     4381 ggatccgcac aaacgaaggt ctcacttaat cttctgtact ctgaagagga gtgggaaata
     4441 ccaagaaaaa catcaaactc gaatgatttt cccaaacccc taccacaaga tattcatcag
     4501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4801 agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa
     4861 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4921 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4981 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5041 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5161 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     5221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5281 cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5341 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5401 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5461 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5521 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5581 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5641 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5701 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5761 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5821 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5881 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5941 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6001 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6061 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6121 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     6181 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg gtcatggctg
     6241 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6301 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6361 catcaccgaa acgcgcgagg cagaaggaga tggcgcccaa cagtcccccg gccacggggc
     6421 ctgcc
//
