LOCUS       sequence-213587-        5450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the insect cell expression system, acceptor for plasmid 
            fusions, two promoters, Multiplication Module M, Tn7 transposition 
            and LoxP site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, 
            Berger I
  TITLE     Protein complex expression by using multigene baculoviral vectors.
  JOURNAL   Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983.
  PUBMED    17117155
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-12"; volume: "3"; issue: "12"; pages: 
            "1021-32"
COMMENT     Sequence Label: pFL
FEATURES             Location/Qualifiers
     source          1..5450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      95..549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(182..201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     391..412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        575..679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(680..748,749..1540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(898..917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1711..2299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2200..2219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2453..2470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2604..2828
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2895..3428)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3617..3622,3623..3639,3640..3645))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(4041..4074)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4264..4312
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4337..4356
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(4448..4557)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4602..4693
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4618..4635
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4952..5086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4989..5008)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5043..5062
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5196..5361
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata
       61 tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      121 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      181 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      241 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      301 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      361 cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact ggaacaacac
      421 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      481 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      541 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      661 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      721 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      781 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      841 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      901 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      961 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1021 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1081 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1141 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1201 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1261 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1321 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1381 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1441 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1501 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1561 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1621 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1681 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1741 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1801 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactattct
     1861 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1921 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1981 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2041 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2101 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2161 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2221 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2281 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2341 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2401 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2461 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2521 tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata
     2581 aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact gaacaaaata
     2641 gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac tgaaatcagt
     2701 ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt
     2761 ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc atggtaaaga
     2821 ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg
     2881 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
     2941 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
     3001 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
     3061 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta
     3121 cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg
     3181 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag
     3241 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga
     3301 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata
     3361 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg
     3421 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     3481 cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc
     3541 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     3601 acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg tttgtgcctt
     3661 catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt
     3721 tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc
     3781 ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc ttcaggagat
     3841 cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg
     3901 catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc
     3961 tagtggttgg ctacagcttt gtttgtacta tcaacaggtt gaactgctga tcaacagatc
     4021 ctctacgcgg ccgcggtacc ataacttcgt atagcataca ttatacgaag ttatctggtt
     4081 taaacgtacc cgtagtggct atggcagggc ttgccgcccc gacgttggct gcgagccctg
     4141 ggccttcacc cgaacttggg ggttggggtg gggaaaagga agaaacgcgg gcgtattggt
     4201 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
     4261 tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
     4321 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccggta
     4381 ccgcatgcta tgcatcagct gctagcacca tggctcgaga tcccgggtga tcaagtcttc
     4441 gtcgagtgat tgtaaataaa atgtaattta cagtatagta ttttaattaa tatacaaatg
     4501 atttgataat aattcttatt taactataat atattgtgtt gggttgaatt aaaggtccgt
     4561 atactagtat cgattcgcga cctactccgg aatattaata gatcatggag ataattaaaa
     4621 tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa
     4681 aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg gatcccggtc
     4741 cgaagcgcgc ggaattcaaa ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga
     4801 atctagagcc tgcagtctcg acaagcttgt cgagaagtac tagaggatca taatcagcca
     4861 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     4921 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     4981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga
     5101 gcctagaaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     5161 ctgagcaata actatcataa cccctaggag atccgaacca gataagtgaa atctagttcc
     5221 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc
     5281 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc
     5341 caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca
     5401 tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg
//
