LOCUS       sequence-213589-        5267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the insect cell expression system, two promoters, 
            Multiplication Module M, Tn7 transposition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, 
            Berger I
  TITLE     Protein complex expression by using multigene baculoviral vectors.
  JOURNAL   Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983.
  PUBMED    17117155
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-12"; volume: "3"; issue: "12"; pages: 
            "1021-32"
COMMENT     Sequence Label: pFBDM
FEATURES             Location/Qualifiers
     source          1..5267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      106..560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(193..212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     402..423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        586..690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(691..759,760..1551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(909..928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1722..2310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2211..2230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2464..2481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2615..2839
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2906..3439)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3628..3633,3634..3650,3651..3656))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4173..4221
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4246..4265
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(4357..4466)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4511..4602
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4527..4544
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    4861..4995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4898..4917)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4952..4971
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5024..5189
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa
       61 ttgactgaat atcaacgctt atttgcagcc tgaatggcga atgggacgcg ccctgtagcg
      121 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      181 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      241 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      301 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
      361 cggtttttcg ccctttgacg ttggagtcca cgttcttaat agtggactct tgttccaaac
      421 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      481 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      541 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      661 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      721 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      781 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      841 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      901 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      961 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1021 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1081 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1141 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1201 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1261 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1321 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1381 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1441 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1501 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1561 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1621 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1681 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1741 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1801 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1861 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1921 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1981 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2041 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2101 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2161 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2221 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2281 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2341 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2401 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2461 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     2521 catctgtgcg gtatttcaca ccgcatagac cagccgcgta acctggcaaa atcggttacg
     2581 gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg cggacaataa agtcttaaac
     2641 tgaacaaaat agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa
     2701 ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca
     2761 aactcttcat tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg
     2821 catggtaaag actatattcg cggcgttgtg acaatttacc gaacaactcc gcggccggga
     2881 agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca
     2941 tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat
     3001 ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga
     3061 tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat
     3121 agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa
     3181 ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga
     3241 ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga
     3301 aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa
     3361 tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat
     3421 cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     3481 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc
     3541 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     3601 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
     3661 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa
     3721 gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt
     3781 caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgcccttg
     3841 cttcaggaga tcggtagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat
     3901 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct
     3961 agctatagtt ctagtggttg gctacagctt tgtttaaaca aagctggcta tggcagggct
     4021 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg gttggggtgg
     4081 ggaaaaggaa gaaacgcggg cgtattggtc ccaatggggt ctcggtgggg tatcgacaga
     4141 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac ccgtgcgttt
     4201 tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg
     4261 tttcagttag cctcccccat ctcccggtac cgcatgctat gcatcagctg ctagcaccat
     4321 ggctcgagat cccgggtgat caagtcttcg tcgagtgatt gtaaataaaa tgtaatttac
     4381 agtatagtat tttaattaat atacaaatga tttgataata attcttattt aactataata
     4441 tattgtgttg ggttgaatta aaggtccgta tactagtatc gattcgcgac ctactccgga
     4501 atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt
     4561 tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac
     4621 cgtcccacca tcgggcgcgg atcccggtcc gaagcgcgcg gaattcaaag gcctacgtcg
     4681 acgagctcac tagtcgcggc cgctttcgaa tctagagcct gcagtctcga caagcttgtc
     4741 gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4801 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4861 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4921 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4981 tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga taagtgaaat
     5041 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
     5101 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
     5161 ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta
     5221 atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggcta
//
