LOCUS       sequence-213664-       10076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. 
            WQDP (199-202) in the first WW domain was changed to AQDA, and the 
            WLDP (258-261) of the second WW domain was changed to ALDA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Ege N, Dowbaj AM, Jiang M, Howell M, Hooper S, Foster C, Jenkins RP,
            Sahai E
  TITLE     Quantitative Analysis Reveals that Actin and Src-Family Kinases 
            Regulate Nuclear YAP1 and Its Export.
  JOURNAL   Cell Syst. 2018 Jun 27;6(6):692-708.e13. doi: 
            10.1016/j.cels.2018.05.006. Epub 2018 Jun 18.
  PUBMED    29909276
REFERENCE   2  (bases 1 to 10076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2018.05.006"; journalName: "Cell Syst"; date: 
            "2018-06-27- 27"; volume: "6"; issue: "6"; pages: "692-708.e13"
COMMENT     Sequence Label: pLL3.7-EF-EYFP-YAP1_WW-PolyA
FEATURES             Location/Qualifiers
     source          1..10076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1865..1909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2398..2414
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2456..2489)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2553..3731
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2784..3722
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3679..3699
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3811..3820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3817..3819,3820..3822,3823..4533)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3862..3883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4123..4142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4470..4491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    4543..4567
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6083..6107)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6500..6581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6537..6556)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6591..6610
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(6639..6672)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6728..7316
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6781..6801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7199..7210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7319..7335)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7845..8025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8087..8675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8167..8186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8846..9637,9638..9706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9469..9488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9707..9811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9886..9905)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     1861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     1921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     1981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2161 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gaacgcgcgg tgaccctcga
     2401 ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg acctaataac
     2461 ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag cggccggccg
     2521 cgtggataac cgtattaccg ccataatcgt gaggctccgg tgcccgtcag tgggcagagc
     2581 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     2641 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     2701 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     2761 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     2821 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     2881 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     2941 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     3001 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3061 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3121 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3181 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3241 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3301 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3361 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3421 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3481 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     3541 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     3601 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     3661 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     3721 aggtgtcgtg aggaattagc ttgcttacat ttgcttctga cacaactgtg ttcactagca
     3781 acctcaaaca gacagctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
     3841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3901 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
     4021 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4081 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4141 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4201 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4261 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     4321 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4381 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
     4441 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4501 gggatcactc tcggcatgga cgagctgtac aagtccggat caacaagttt gtacaaaaaa
     4561 gcaggcttgg atcccgggca gcagccgccg cctcaaccgg ccccccaggg ccaagggcag
     4621 ccgccttcgc agcccccgca ggggcagggc ccgccgtccg gacccgggca accggcaccc
     4681 gcggcgaccc aggcggcgcc gcaggcaccc cccgccgggc atcagatcgt gcacgtccgc
     4741 ggggactcgg agaccgacct ggaggcgctc ttcaacgccg tcatgaaccc caagacggcc
     4801 aacgtgcccc agaccgtgcc catgaggctc cggaagctgc ccgactcctt cttcaagccg
     4861 ccggagccca aatcccactc ccgacaggcc agtactgatg caggcactgc aggagccctg
     4921 actccacagc atgttcgagc tcattcctct ccagcttctc tgcagttggg agctgtttct
     4981 cctgggacac tgacccccac tggagtagtc tctggcccag cagctacacc cacagctcag
     5041 catcttcgac agtcttcttt tgagatacct gatgatgtac ctctgccagc aggttgggag
     5101 atggcaaaga catcttctgg tcagagatac ttcttaaatc acatcgatca gacaacaaca
     5161 gcccaggacg ccaggaaggc catgctgtcc cagatgaacg tcacagcccc caccagtcca
     5221 ccagtgcagc agaatatgat gaactcggct tcaggtcctc ttcctgatgg atgggaacaa
     5281 gccatgactc aggatggaga aatttactat ataaaccata agaacaagac cacctctgcc
     5341 ctagacgcca ggcttgaccc tcgttttgcc atgaaccaga gaatcagtca gagtgctcca
     5401 gtgaaacagc caccacccct ggctccccag agcccacagg gaggcgtcat gggtggcagc
     5461 aactccaacc agcagcaaca gatgcgactg cagcaactgc agatggagaa ggagaggctg
     5521 cggctgaaac agcaagaact gcttcggcag gcaatgcgga atatcaatcc cagcacagca
     5581 aattctccaa aatgtcagga gttagccctg cgtagccagt taccaacact ggagcaggat
     5641 ggtgggactc aaaatccagt gtcttctccc gggatgtctc aggaattgag aacaatgacg
     5701 accaatagct cagatccttt ccttaacagt ggcacctatc actctcgaga tgagagtaca
     5761 gacagtggac taagcatgag cagctacagt gtccctcgaa ccccagatga cttcctgaac
     5821 agtgtggatg agatggatac aggtgatact atcaaccaaa gcaccctgcc ctcacagcag
     5881 aaccgtttcc cagactacct tgaagccatt cctgggacaa atgtggacct tggaacactg
     5941 gaaggagatg gaatgaacat agaaggagag gagctgatgc caagtctgca ggaagctttg
     6001 agttctgaca tccttaatga catggagtct gttttggctg ccaccaagct agataaagaa
     6061 agctttctta catggttata ggacccagct ttcttgtaca aagtggttga tccaccggat
     6121 ctagataact gatcataatc agccatacca catttgatcg aggattacca ctatgctacg
     6181 tgtacaaacg aggacctgat gcactttgac gacttgggat cgacgagagc agcgcgactg
     6241 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     6301 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     6361 ttgttgttgg ccgcggatcc gtttaaacga attcatcata atcagccata ccacatttgt
     6421 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     6481 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     6541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6601 caaactcatc aatgtatccc aattcgtcga gggacctaat aacttcgtat agcatacatt
     6661 atacgaagtt atacatgttt aagggttccg gttccactag gtacaattcg atatcaagct
     6721 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6781 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6841 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6901 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6961 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7021 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7081 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7141 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7201 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7261 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7321 ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     7381 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     7441 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7501 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     7561 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     7621 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7681 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     7741 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     7801 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     7861 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7921 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7981 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatg tgagcaaaag
     8041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8521 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8701 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8821 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8881 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8941 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9001 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9061 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9841 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     9901 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     9961 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
    10021 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
