LOCUS       sequence-213665-        7897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and 
            targeted to the neuronal soma and proximal dendrites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov
            K, Brohawn SG, Waller L, Adesnik H
  TITLE     Precise multimodal optical control of neural ensemble activity.
  JOURNAL   Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8.
  PUBMED    29713079
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 
            10.1038/s41593-018-0139-8."
COMMENT     Sequence Label: pCAG-C1V1-TT-mRuby2-ST
FEATURES             Location/Qualifiers
     source          1..7897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2859..3569
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     intron          3793..3925
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3908..3927
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    3934..4522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3987..4007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4405..4416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4549..4566)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4555..4666
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4762..4782)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4762..4780)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4801..4817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4919..4938)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4984..5039
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5038..5057)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5400..5416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5424..5440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5448..5454,5455..5472,5473..5478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5493..5514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5573..5768
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5619..5754
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5774..5908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5811..5830)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5865..5884
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5976..5993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6147..6735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6227..6246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6906..7697,7698..7766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7529..7548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7767..7871)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcgggggggg gcgggggggg
      541 ggggggggcg gggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcggtaccca
     1741 ccatgtcgcg gaggccatgg cttcttgccc tagcgctggc agtggcgctg gcggccggca
     1801 gcgcaggagc ctcgactggc agtgacgcga cggtgccggt cgcgactcag gatggccccg
     1861 actacgtttt ccaccgtgcc cacgagcgca tgctcttcca aacctcatac actcttgaga
     1921 acaatggttc tgttatttgc atcccgaaca acggccagtg cttctgcttg gcttggctta
     1981 aatccaacgg aacaaatgcc gagaagttgg ctgccaacat tctgcagtgg attacttttg
     2041 cgctttcagc gctctgcctg atgttctacg gctaccagac ctggaagtct acttgcggct
     2101 gggagaccat ttacgtggcc acgatcgaga tgatcaagtt catcatcgag tatttccatg
     2161 agtttgacga acctgcggtg atctactcat ccaacggcaa caagaccgtg tggcttcgtt
     2221 acgcgacctg gctgctcacc tgcccggtcc ttctgatcca tctgagcaac ctcacaggcc
     2281 tgaaggacga ttatagcaaa aggactatgg gcctgttggt ttctgatgtg ggatgcatcg
     2341 tgtggggcgc aaccagcgcc atgtgtacgg ggtggacgaa gatcctgttc ttcctcatct
     2401 cattgagcta tggtatgtat acctattttc atgctgctaa agtttatatc gaagcattcc
     2461 acacagttcc aaaagggatt tgtcgagaac tggtccgagt gatggcctgg acattctttg
     2521 tggcttgggg aatgtttcca gtcctgtttc tgctgggcac ggaaggattc ggtcatatca
     2581 gcccttatgg atctgccatt gggcactcca tcctcgacct gattgcaaag aacatgtggg
     2641 gtgtgctggg gaattacctg cgcgtcaaaa tccacgagca catcctgttg tatggcgaca
     2701 tcagaaagaa gcagaaaatt acgatcgccg gccaagagat ggaggttgag acactggtgg
     2761 ctgaagagga ggacgcggcc gccaagagca ggatcaccag cgagggcgag tacatccccc
     2821 tggaccagat cgacatcaac gtggcggccg caccggtaat ggtgtctaag ggcgaagagc
     2881 tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat
     2941 tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc atgaggatca
     3001 aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt
     3061 atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt aaacagtcct
     3121 ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg
     3181 tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa gtcagagggg
     3241 taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata
     3301 cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg gcactgaaag
     3361 ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg
     3421 tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa aggttagagg
     3481 aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag ttcgccgggc
     3541 ttggtggtgg gatggacgag ctgtacaagg ccctagagca gtcccagccc atcctcaaca
     3601 ccaaggagat ggccccgcag agcaagcctc cagaggagct ggagatgagc agcatgccca
     3661 gccccgtggc ccctctgccc gcacgcacgg agggcgtcat cgacatgcgg agcatgtcca
     3721 gcattgacag cttcatcagc tgtgccacgg acttccctga agccaccaga ttctaaggac
     3781 tcgaggccgc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
     3841 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
     3901 atccactttg cctttctctc cacaggtgtc gacaatcaac ctctggatta caaaatttgt
     3961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4321 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4501 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acgatcagcc tcgactgtgc
     4561 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4621 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4681 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4741 acaatagccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     4801 gtcatagctg tttcctgtgt gaaattgtta tccgctaatt cactcctcag gtgcaggctg
     4861 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     4921 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     4981 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     5041 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     5101 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     5161 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     5221 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     5281 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     5341 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     5401 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5461 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5521 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     5581 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5641 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5701 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5761 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5821 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5881 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     5941 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6001 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6061 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6781 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6841 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6901 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6961 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7021 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7081 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7141 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7201 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7261 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7321 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7381 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7441 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7501 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7561 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7621 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7681 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7741 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7801 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7861 ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
