LOCUS       sequence-213684-        7542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 197-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 197-972
FEATURES             Location/Qualifiers
     source          1..7542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     119..141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     133..150
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     134..150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     160..179
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        160..178
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3058..3078)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3058..3076)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3097..3113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3097..3113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3110..3132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3121..3137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3145..3151,3152..3169,3170..3175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3190..3211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3328..3345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3499..4087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3579..3598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4258..5049,5050..5118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4881..4900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5119..5223)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5260..5763
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5804..5822
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5860..5882)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5982..6001
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6022..6302
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6303..6977
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7080..7535)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7217..7238)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7429..7448
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
       61 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      121 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
      181 gaattgggta ccgggccccc ccctcgagca tgtactttcc gcctaactac catcacctgt
      241 ctttatttat tccgctgaca gatatgcaca accaattatc gtctcttgaa agctttttag
      301 acgtgattgc tgttatttgg cacgttgtta gtatttaagc ttattcgggt ttgcattgat
      361 atttgcccga gcagaaatct ccttctcgat gagttagaac tgaaacatct cttcaattac
      421 ttatacataa taaacataga aagccaggac ctgtcatcat gccagaaatg atatttaacc
      481 gcttattgcc catattacta gacagaagtt tggaagacca ggaaagacat ctaatgatca
      541 agaccataga tcgcgtcctt taccagctag gagatttaac aaaaccttac gttcacaaaa
      601 tacttgtagt agctgcccca ttgctaatag atgaagatcc aatggttcgt tcaacaggcc
      661 aggagataat caccaatctg tctacagtcg ctggcctaaa aaccattcta acagtaatga
      721 gacctgatat tgagaatgag gatgagtatg ttagaaatgt aacttcaaga gcggcggctg
      781 tggtggcaaa agcccttggt gttaatcaat tgcttccttt catcaatgca gcatgccact
      841 cacggaagtc ttggaaagca aggcatactg gcataaaaat tgtgcagcaa ataggtatac
      901 ttttgggaat aggtgtcttg aatcatctta ctgggctgat gagttgtatc aaagactgcc
      961 taatggatga tcacgttccg gtgcgaattg ttacagcaca tactttatca acactagcag
     1021 aaaattctta tccatatggt atcgaagttt tcaacgttgt actggagcca ttatggaagg
     1081 gtataagaag tcatcgtggt aaagtgctat cttctttttt aaaagctgtt ggatctatga
     1141 tcccattgat ggacccagag tatgccggtt attataccac agaagcaatg agaataatta
     1201 gacgtgaatt cgactctccc gatgatgaaa tgaaaaagac cattctttta gtattacaaa
     1261 aatgtagcgc tgtagaatcc ataacgccaa aatttctgag agaagaaatc gcgccagaat
     1321 tctttcaaaa attttgggtc agacgagtgg cattggatag accattaaac aaagttgtta
     1381 catatactac tgttacatta gcaaagaaat taggttgttc ttataccatt gataaattat
     1441 taacaccatt gagagatgaa gctgagccgt tcagaacaat ggctgtccat gctgttacta
     1501 gaacagtaaa cttattaggg acagcagatt tagatgagag attagaaacg aggcttattg
     1561 atgcgctctt gatagcattt caagaacaaa caaatagtga ttctataatc tttaaagggt
     1621 ttggggcagt gacggtgtca cttgacatcc gtatgaaacc gtttttagca cccattgtga
     1681 gtacgatttt aaatcattta aagcataaaa ctcccttggt tcgtcaacat gcagcagatc
     1741 tatgtgccat actaatacca gtcatcaaga actgtcacga attcgaaatg ttgaataaat
     1801 tgaacattat attgtatgaa tccttaggcg aagtatatcc agaggtatta ggttctataa
     1861 tcaacgctat gtactgtatt actagcgtaa tggatttgga taaactacaa cctcctatta
     1921 atcaaatttt accaacctta actccaattt taagaaacaa gcatagaaaa gtcgaagtaa
     1981 acactatcaa atttgttgga cttattggta aattagcacc tacctatgca cctcctaaag
     2041 aatggatgag aatatgtttt gaactattag aacttttgaa aagtacaaat aaagaaataa
     2101 gaagatcagc aaatgccacg tttggattca ttgctgaagc aattggacct cacgatgttc
     2161 ttgttgcgtt attgaacaat ttaaaggttc aagaacgtca gttacgtgta tgcacggctg
     2221 ttgctatcgg tatcgttgct aaagtgtgtg gtccttacaa cgtgctgcca gtaatcatga
     2281 atgaatacac aacaccagag actaatgttc aaaacggtgt tctcaaggcc atgtcattca
     2341 tgtttgagta cattggtaac atgtctaagg attacatata cttcataaca ccattattag
     2401 aagatgcact caccgatagg gatttagttc atcgtcagac agcgtcgaac gtaataaccc
     2461 acttagcttt aaactgttca ggtacaggtc acgaagatgc cttcattcat ttaatgaatc
     2521 ttctaatacc caatattttt gaaacttcac cacatgctat tatgcgtatt ttagaaggtt
     2581 tggaggcact gagtcaagct cttggccctg gactattcat gaactacata tgggcaggat
     2641 tgttccatcc agcgaagaat gtaaggaaag cattttggag ggtgtataat aacatgtacg
     2701 taatgtacca ggacgccatg gtaccttttt accccgttac accagacaac aatgaagaat
     2761 atatagaaga actggattta gttctgtgaa tatatataga gcatataaat aaccacaact
     2821 tgtaagaata ttttacttga cacacctata tcatcacgtc acgatgcctt catagattat
     2881 gatgaaatat aaacagaacc tgctattgga aaaaaaaggg caggaaaaaa ttttgtgaaa
     2941 gtgagtaaaa gaaagatgaa gagcgagaca tggataataa gaaatagatg tcactaatgg
     3001 acaattggaa gactgatatg gaaagttacg atgaaggagg agctccagct tttgttccct
     3061 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3121 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3181 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3241 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3301 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3901 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4141 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4201 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4261 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4321 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4381 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4441 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4501 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4561 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4621 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4681 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4741 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4801 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4861 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4921 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4981 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5041 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5101 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5161 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5221 gcgcacattt ccccgaaaag tgccacctgg gtccttttca tcacgtgcta taaaaataat
     5281 tataatttaa attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat
     5341 taaagaaaaa atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca
     5401 aatactacct tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct
     5461 tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat
     5521 cgaatgtata tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct
     5581 ttttgttgaa attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta
     5641 ccttctttat ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt
     5701 aaattcccaa attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg
     5761 atccgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     5821 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5881 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5941 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     6001 actgagagtg caccatagat cacgacatta ctatatatat aatataggaa gcatttaata
     6061 gaacagcatc gtaatatatg tgtactttgc agttatgacg ccagatggca gtagtggaag
     6121 atattcttta ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg gagcttttct
     6181 ttttttgccg attaagaatt aattcggtcg aaaaaagaaa aggagagggc caagagggag
     6241 ggcattggtg actattgagc acgtgagtat acgtgattaa gcacacaaag gcagcttgga
     6301 gtatgtctgt tattaatttc acaggtagtt ctggtccatt ggtgaaagtt tgcggcttgc
     6361 agagcacaga ggccgcagaa tgtgctctag attccgatgc tgacttgctg ggtattatat
     6421 gtgtgcccaa tagaaagaga acaattgacc cggttattgc aaggaaaatt tcaagtcttg
     6481 taaaagcata taaaaatagt tcaggcactc cgaaatactt ggttggcgtg tttcgtaatc
     6541 aacctaagga ggatgttttg gctctggtca atgattacgg cattgatatc gtccaactgc
     6601 atggagatga gtcgtggcaa gaataccaag agttcctcgg tttgccagtt attaaaagac
     6661 tcgtatttcc aaaagactgc aacatactac tcagtgcagc ttcacagaaa cctcattcgt
     6721 ttattccctt gtttgattca gaagcaggtg ggacaggtga acttttggat tggaactcga
     6781 tttctgactg ggttggaagg caagagagcc ccgaaagctt acattttatg ttagctggtg
     6841 gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg
     6901 taagcggagg tgtggagaca aatggtgtaa aagactctaa caaaatagca aatttcgtca
     6961 aaaatgctaa gaaataggtt attactgagt agtatttatt taagtattgt ttgtgcactt
     7021 gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     7081 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7141 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7201 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     7261 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     7321 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     7381 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     7441 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     7501 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cg
//
