LOCUS       sequence-213704-        7661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 157-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 157-972
FEATURES             Location/Qualifiers
     source          1..7661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3044..3064)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3044..3062)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3083..3099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3083..3099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3096..3118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3107..3123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3131..3137,3138..3155,3156..3161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3176..3197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3314..3331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3485..4073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3565..3584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4244..5035,5036..5104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4867..4886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5105..5209)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5246..5749
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5790..5808
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5846..5868)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5968..5987
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6008..6288
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6289..6963
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(7066..7521)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7203..7224)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7415..7434
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7647..7661,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7661,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgaa aaggatgaaa agaaagaaag aaccctgtcg
      361 atgttacttc tgaaaataaa aaatggcaat acagcgagta ggaggacttc tatgcggatt
      421 ttaaccgata aagctgttac atttggtcca gaaatgatat ttaaccgctt attgcccata
      481 ttactagaca gaagtttgga agaccaggaa agacatctaa tgatcaagac catagatcgc
      541 gtcctttacc agctaggaga tttaacaaaa ccttacgttc acaaaatact tgtagtagct
      601 gccccattgc taatagatga agatccaatg gttcgttcaa caggccagga gataatcacc
      661 aatctgtcta cagtcgctgg cctaaaaacc attctaacag taatgagacc tgatattgag
      721 aatgaggatg agtatgttag aaatgtaact tcaagagcgg cggctgtggt ggcaaaagcc
      781 cttggtgtta atcaattgct tcctttcatc aatgcagcat gccactcacg gaagtcttgg
      841 aaagcaaggc atactggcat aaaaattgtg cagcaaatag gtatactttt gggaataggt
      901 gtcttgaatc atcttactgg gctgatgagt tgtatcaaag actgcctaat ggatgatcac
      961 gttccggtgc gaattgttac agcacatact ttatcaacac tagcagaaaa ttcttatcca
     1021 tatggtatcg aagttttcaa cgttgtactg gagccattat ggaagggtat aagaagtcat
     1081 cgtggtaaag tgctatcttc ttttttaaaa gctgttggat ctatgatccc attgatggac
     1141 ccagagtatg ccggttatta taccacagaa gcaatgagaa taattagacg tgaattcgac
     1201 tctcccgatg atgaaatgaa aaagaccatt cttttagtat tacaaaaatg tagcgctgta
     1261 gaatccataa cgccaaaatt tctgagagaa gaaatcgcgc cagaattctt tcaaaaattt
     1321 tgggtcagac gagtggcatt ggatagacca ttaaacaaag ttgttacata tactactgtt
     1381 acattagcaa agaaattagg ttgttcttat accattgata aattattaac accattgaga
     1441 gatgaagctg agccgttcag aacaatggct gtccatgctg ttactagaac agtaaactta
     1501 ttagggacag cagatttaga tgagagatta gaaacgaggc ttattgatgc gctcttgata
     1561 gcatttcaag aacaaacaaa tagtgattct ataatcttta aagggtttgg ggcagtgacg
     1621 gtgtcacttg acatccgtat gaaaccgttt ttagcaccca ttgtgagtac gattttaaat
     1681 catttaaagc ataaaactcc cttggttcgt caacatgcag cagatctatg tgccatacta
     1741 ataccagtca tcaagaactg tcacgaattc gaaatgttga ataaattgaa cattatattg
     1801 tatgaatcct taggcgaagt atatccagag gtattaggtt ctataatcaa cgctatgtac
     1861 tgtattacta gcgtaatgga tttggataaa ctacaacctc ctattaatca aattttacca
     1921 accttaactc caattttaag aaacaagcat agaaaagtcg aagtaaacac tatcaaattt
     1981 gttggactta ttggtaaatt agcacctacc tatgcacctc ctaaagaatg gatgagaata
     2041 tgttttgaac tattagaact tttgaaaagt acaaataaag aaataagaag atcagcaaat
     2101 gccacgtttg gattcattgc tgaagcaatt ggacctcacg atgttcttgt tgcgttattg
     2161 aacaatttaa aggttcaaga acgtcagtta cgtgtatgca cggctgttgc tatcggtatc
     2221 gttgctaaag tgtgtggtcc ttacaacgtg ctgccagtaa tcatgaatga atacacaaca
     2281 ccagagacta atgttcaaaa cggtgttctc aaggccatgt cattcatgtt tgagtacatt
     2341 ggtaacatgt ctaaggatta catatacttc ataacaccat tattagaaga tgcactcacc
     2401 gatagggatt tagttcatcg tcagacagcg tcgaacgtaa taacccactt agctttaaac
     2461 tgttcaggta caggtcacga agatgccttc attcatttaa tgaatcttct aatacccaat
     2521 atttttgaaa cttcaccaca tgctattatg cgtattttag aaggtttgga ggcactgagt
     2581 caagctcttg gccctggact attcatgaac tacatatggg caggattgtt ccatccagcg
     2641 aagaatgtaa ggaaagcatt ttggagggtg tataataaca tgtacgtaat gtaccaggac
     2701 gccatggtac ctttttaccc cgttacacca gacaacaatg aagaatatat agaagaactg
     2761 gatttagttc tgtgaatata tatagagcat ataaataacc acaacttgta agaatatttt
     2821 acttgacaca cctatatcat cacgtcacga tgccttcata gattatgatg aaatataaac
     2881 agaacctgct attggaaaaa aaagggcagg aaaaaatttt gtgaaagtga gtaaaagaaa
     2941 gatgaagagc gagacatgga taataagaaa tagatgtcac taatggacaa ttggaagact
     3001 gatatggaaa gttacgatga aggaggagct ccagcttttg ttccctttag tgagggttaa
     3061 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     3301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3901 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5221 gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt
     5281 tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag
     5341 tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta
     5401 tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag
     5461 accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta
     5521 tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt
     5581 tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac
     5641 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
     5701 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa
     5761 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     5821 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5881 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5941 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     6001 atagatcacg acattactat atatataata taggaagcat ttaatagaac agcatcgtaa
     6061 tatatgtgta ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga
     6121 aaaatagctt gtcaccttac gtacaatctt gatccggagc ttttcttttt ttgccgatta
     6181 agaattaatt cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta
     6241 ttgagcacgt gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt
     6301 aatttcacag gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc
     6361 gcagaatgtg ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga
     6421 aagagaacaa ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa
     6481 aatagttcag gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat
     6541 gttttggctc tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg
     6601 tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa
     6661 gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt
     6721 gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt
     6781 ggaaggcaag agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa
     6841 aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg
     6901 gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa
     6961 taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgccg atctatgcgg
     7021 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     7081 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     7141 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     7201 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     7261 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     7321 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     7381 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     7441 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     7501 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     7561 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     7621 attaagttgg gtaacgccag ggttttccca gtcacgacgt t
//
