LOCUS       sequence-213707-        7308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 275-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 275-972
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2691..2711)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2691..2709)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2730..2746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2730..2746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2743..2765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2754..2770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2778..2784,2785..2802,2803..2808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2823..2844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2961..2978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3132..3720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3212..3231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3891..4682,4683..4751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4514..4533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4752..4856)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4893..5396
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5437..5455
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5493..5515)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5615..5634
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5655..5935
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5936..6610
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6713..7168)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6850..6871)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7062..7081
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7294..7308,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7308,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatgct aaaaaccatt ctaacagtaa tgagacctga
      361 tattgagaat gaggatgagt atgttagaaa tgtaacttca agagcggcgg ctgtggtggc
      421 aaaagccctt ggtgttaatc aattgcttcc tttcatcaat gcagcatgcc actcacggaa
      481 gtcttggaaa gcaaggcata ctggcataaa aattgtgcag caaataggta tacttttggg
      541 aataggtgtc ttgaatcatc ttactgggct gatgagttgt atcaaagact gcctaatgga
      601 tgatcacgtt ccggtgcgaa ttgttacagc acatacttta tcaacactag cagaaaattc
      661 ttatccatat ggtatcgaag ttttcaacgt tgtactggag ccattatgga agggtataag
      721 aagtcatcgt ggtaaagtgc tatcttcttt tttaaaagct gttggatcta tgatcccatt
      781 gatggaccca gagtatgccg gttattatac cacagaagca atgagaataa ttagacgtga
      841 attcgactct cccgatgatg aaatgaaaaa gaccattctt ttagtattac aaaaatgtag
      901 cgctgtagaa tccataacgc caaaatttct gagagaagaa atcgcgccag aattctttca
      961 aaaattttgg gtcagacgag tggcattgga tagaccatta aacaaagttg ttacatatac
     1021 tactgttaca ttagcaaaga aattaggttg ttcttatacc attgataaat tattaacacc
     1081 attgagagat gaagctgagc cgttcagaac aatggctgtc catgctgtta ctagaacagt
     1141 aaacttatta gggacagcag atttagatga gagattagaa acgaggctta ttgatgcgct
     1201 cttgatagca tttcaagaac aaacaaatag tgattctata atctttaaag ggtttggggc
     1261 agtgacggtg tcacttgaca tccgtatgaa accgttttta gcacccattg tgagtacgat
     1321 tttaaatcat ttaaagcata aaactccctt ggttcgtcaa catgcagcag atctatgtgc
     1381 catactaata ccagtcatca agaactgtca cgaattcgaa atgttgaata aattgaacat
     1441 tatattgtat gaatccttag gcgaagtata tccagaggta ttaggttcta taatcaacgc
     1501 tatgtactgt attactagcg taatggattt ggataaacta caacctccta ttaatcaaat
     1561 tttaccaacc ttaactccaa ttttaagaaa caagcataga aaagtcgaag taaacactat
     1621 caaatttgtt ggacttattg gtaaattagc acctacctat gcacctccta aagaatggat
     1681 gagaatatgt tttgaactat tagaactttt gaaaagtaca aataaagaaa taagaagatc
     1741 agcaaatgcc acgtttggat tcattgctga agcaattgga cctcacgatg ttcttgttgc
     1801 gttattgaac aatttaaagg ttcaagaacg tcagttacgt gtatgcacgg ctgttgctat
     1861 cggtatcgtt gctaaagtgt gtggtcctta caacgtgctg ccagtaatca tgaatgaata
     1921 cacaacacca gagactaatg ttcaaaacgg tgttctcaag gccatgtcat tcatgtttga
     1981 gtacattggt aacatgtcta aggattacat atacttcata acaccattat tagaagatgc
     2041 actcaccgat agggatttag ttcatcgtca gacagcgtcg aacgtaataa cccacttagc
     2101 tttaaactgt tcaggtacag gtcacgaaga tgccttcatt catttaatga atcttctaat
     2161 acccaatatt tttgaaactt caccacatgc tattatgcgt attttagaag gtttggaggc
     2221 actgagtcaa gctcttggcc ctggactatt catgaactac atatgggcag gattgttcca
     2281 tccagcgaag aatgtaagga aagcattttg gagggtgtat aataacatgt acgtaatgta
     2341 ccaggacgcc atggtacctt tttaccccgt tacaccagac aacaatgaag aatatataga
     2401 agaactggat ttagttctgt gaatatatat agagcatata aataaccaca acttgtaaga
     2461 atattttact tgacacacct atatcatcac gtcacgatgc cttcatagat tatgatgaaa
     2521 tataaacaga acctgctatt ggaaaaaaaa gggcaggaaa aaattttgtg aaagtgagta
     2581 aaagaaagat gaagagcgag acatggataa taagaaatag atgtcactaa tggacaattg
     2641 gaagactgat atggaaagtt acgatgaagg aggagctcca gcttttgttc cctttagtga
     2701 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2761 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2821 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2881 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2941 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3001 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3061 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3421 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3601 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3841 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4501 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4681 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4741 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4861 tttccccgaa aagtgccacc tgggtccttt tcatcacgtg ctataaaaat aattataatt
     4921 taaatttttt aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa
     4981 aaaatagttt ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta
     5041 ccttttatct tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt
     5101 gtagaagacc acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt
     5161 atatctattt aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt
     5221 gaaatttttt aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt
     5281 tatttacttt ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc
     5341 caaattattc catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc
     5401 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5461 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5521 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5581 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5641 gtgcaccata gatcacgaca ttactatata tataatatag gaagcattta atagaacagc
     5701 atcgtaatat atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct
     5761 ttattgaaaa atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg
     5821 ccgattaaga attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg
     5881 gtgactattg agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc
     5941 tgttattaat ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac
     6001 agaggccgca gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc
     6061 caatagaaag agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc
     6121 atataaaaat agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa
     6181 ggaggatgtt ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga
     6241 tgagtcgtgg caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt
     6301 tccaaaagac tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc
     6361 cttgtttgat tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga
     6421 ctgggttgga aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac
     6481 gccagaaaat gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg
     6541 aggtgtggag acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc
     6601 taagaaatag gttattactg agtagtattt atttaagtat tgtttgtgca cttgccgatc
     6661 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     6721 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     6781 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6841 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6901 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6961 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     7021 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     7081 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     7141 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     7201 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     7261 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgtt
//
