LOCUS       sequence-213709-          75 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses mCherry fused to Lifeact peptide. It labels 
            actin bundles and filopodia in Capsaspora. Codons have been 
            optimised according to their usages in Capsaspora and C. 
            fragrantissima..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 75)
  AUTHORS   Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, 
            Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
  TITLE     Transfection of Capsaspora owczarzaki, a close unicellular relative 
            of animals.
  JOURNAL   Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107.
  PUBMED    29752387
REFERENCE   2  (bases 1 to 75)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2018 May 11. pii: dev.162107. doi: 
            10.1242/dev.162107."
COMMENT     Sequence Label: pONSY-Lifeact:mCherry
FEATURES             Location/Qualifiers
     source          1..75
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..51
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
ORIGIN
        1 atgggtgtgg cagacctgat taagaagttc gagagcatta gcaaggagga gggtgaccca
       61 ccatctagtg atatc
//
