LOCUS       sequence-213710-        7203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 310-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 310-972
FEATURES             Location/Qualifiers
     source          1..7203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2586..2606)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2586..2604)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2625..2641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2625..2641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2638..2660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2649..2665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2673..2679,2680..2697,2698..2703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2718..2739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2856..2873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3027..3615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3107..3126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3786..4577,4578..4646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4409..4428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4647..4751)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4788..5291
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     5332..5350
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5388..5410)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5510..5529
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5550..5830
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5831..6505
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(6608..7063)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6745..6766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6957..6976
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(7189..7203,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(7203,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
       61 ggcccccccc tcgagcatgt actttccgcc taactaccat cacctgtctt tatttattcc
      121 gctgacagat atgcacaacc aattatcgtc tcttgaaagc tttttagacg tgattgctgt
      181 tatttggcac gttgttagta tttaagctta ttcgggtttg cattgatatt tgcccgagca
      241 gaaatctcct tctcgatgag ttagaactga aacatctctt caattactta tacataataa
      301 acatagaaag ccaggacctg tcatcatggt taatcaattg cttcctttca tcaatgcagc
      361 atgccactca cggaagtctt ggaaagcaag gcatactggc ataaaaattg tgcagcaaat
      421 aggtatactt ttgggaatag gtgtcttgaa tcatcttact gggctgatga gttgtatcaa
      481 agactgccta atggatgatc acgttccggt gcgaattgtt acagcacata ctttatcaac
      541 actagcagaa aattcttatc catatggtat cgaagttttc aacgttgtac tggagccatt
      601 atggaagggt ataagaagtc atcgtggtaa agtgctatct tcttttttaa aagctgttgg
      661 atctatgatc ccattgatgg acccagagta tgccggttat tataccacag aagcaatgag
      721 aataattaga cgtgaattcg actctcccga tgatgaaatg aaaaagacca ttcttttagt
      781 attacaaaaa tgtagcgctg tagaatccat aacgccaaaa tttctgagag aagaaatcgc
      841 gccagaattc tttcaaaaat tttgggtcag acgagtggca ttggatagac cattaaacaa
      901 agttgttaca tatactactg ttacattagc aaagaaatta ggttgttctt ataccattga
      961 taaattatta acaccattga gagatgaagc tgagccgttc agaacaatgg ctgtccatgc
     1021 tgttactaga acagtaaact tattagggac agcagattta gatgagagat tagaaacgag
     1081 gcttattgat gcgctcttga tagcatttca agaacaaaca aatagtgatt ctataatctt
     1141 taaagggttt ggggcagtga cggtgtcact tgacatccgt atgaaaccgt ttttagcacc
     1201 cattgtgagt acgattttaa atcatttaaa gcataaaact cccttggttc gtcaacatgc
     1261 agcagatcta tgtgccatac taataccagt catcaagaac tgtcacgaat tcgaaatgtt
     1321 gaataaattg aacattatat tgtatgaatc cttaggcgaa gtatatccag aggtattagg
     1381 ttctataatc aacgctatgt actgtattac tagcgtaatg gatttggata aactacaacc
     1441 tcctattaat caaattttac caaccttaac tccaatttta agaaacaagc atagaaaagt
     1501 cgaagtaaac actatcaaat ttgttggact tattggtaaa ttagcaccta cctatgcacc
     1561 tcctaaagaa tggatgagaa tatgttttga actattagaa cttttgaaaa gtacaaataa
     1621 agaaataaga agatcagcaa atgccacgtt tggattcatt gctgaagcaa ttggacctca
     1681 cgatgttctt gttgcgttat tgaacaattt aaaggttcaa gaacgtcagt tacgtgtatg
     1741 cacggctgtt gctatcggta tcgttgctaa agtgtgtggt ccttacaacg tgctgccagt
     1801 aatcatgaat gaatacacaa caccagagac taatgttcaa aacggtgttc tcaaggccat
     1861 gtcattcatg tttgagtaca ttggtaacat gtctaaggat tacatatact tcataacacc
     1921 attattagaa gatgcactca ccgataggga tttagttcat cgtcagacag cgtcgaacgt
     1981 aataacccac ttagctttaa actgttcagg tacaggtcac gaagatgcct tcattcattt
     2041 aatgaatctt ctaataccca atatttttga aacttcacca catgctatta tgcgtatttt
     2101 agaaggtttg gaggcactga gtcaagctct tggccctgga ctattcatga actacatatg
     2161 ggcaggattg ttccatccag cgaagaatgt aaggaaagca ttttggaggg tgtataataa
     2221 catgtacgta atgtaccagg acgccatggt acctttttac cccgttacac cagacaacaa
     2281 tgaagaatat atagaagaac tggatttagt tctgtgaata tatatagagc atataaataa
     2341 ccacaacttg taagaatatt ttacttgaca cacctatatc atcacgtcac gatgccttca
     2401 tagattatga tgaaatataa acagaacctg ctattggaaa aaaaagggca ggaaaaaatt
     2461 ttgtgaaagt gagtaaaaga aagatgaaga gcgagacatg gataataaga aatagatgtc
     2521 actaatggac aattggaaga ctgatatgga aagttacgat gaaggaggag ctccagcttt
     2581 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     2641 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2701 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2761 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2821 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2881 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2941 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3001 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3061 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3121 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3181 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3241 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3301 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3361 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3421 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3481 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3541 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3601 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3781 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3841 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3901 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3961 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4021 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4081 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4141 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4201 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4261 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4321 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4381 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4441 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4501 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4561 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4621 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt ccttttcatc acgtgctata
     4801 aaaataatta taatttaaat tttttaatat aaatatataa attaaaaata gaaagtaaaa
     4861 aaagaaatta aagaaaaaat agtttttgtt ttccgaagat gtaaaagact ctagggggat
     4921 cgccaacaaa tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg
     4981 tttcatcttg tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta
     5041 tcgttaatcg aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa
     5101 agtacgcttt ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa
     5161 ctcttctacc ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa
     5221 cacagagtaa attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca
     5281 ggcaagcgat ccgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5341 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     5401 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     5461 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     5521 cagattgtac tgagagtgca ccatagatca cgacattact atatatataa tataggaagc
     5581 atttaataga acagcatcgt aatatatgtg tactttgcag ttatgacgcc agatggcagt
     5641 agtggaagat attctttatt gaaaaatagc ttgtcacctt acgtacaatc ttgatccgga
     5701 gcttttcttt ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca
     5761 agagggaggg cattggtgac tattgagcac gtgagtatac gtgattaagc acacaaaggc
     5821 agcttggagt atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg
     5881 cggcttgcag agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg
     5941 tattatatgt gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc
     6001 aagtcttgta aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt
     6061 tcgtaatcaa cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt
     6121 ccaactgcat ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat
     6181 taaaagactc gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc
     6241 tcattcgttt attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg
     6301 gaactcgatt tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt
     6361 agctggtgga ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg
     6421 tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa
     6481 tttcgtcaaa aatgctaaga aataggttat tactgagtag tatttattta agtattgttt
     6541 gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     6601 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6661 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6721 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6781 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6841 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     6901 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     6961 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7021 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     7081 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     7141 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     7201 gtt
//
